Biomolecules & Therapeutics

  • 제25권4호
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  • Pages.417-426
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  • 2017
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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4-O-Methylhonokiol Protects HaCaT Cells from TGF-β1-Induced Cell Cycle Arrest by Regulating Canonical and Non-Canonical Pathways of TGF-β Signaling

  • Kim, Sang-Cheol (Department of Medicine, Jeju National University School of Medicine) ;
  • Kang, Jung-Il (Department of Medicine, Jeju National University School of Medicine) ;
  • Hyun, Jin-Won (Department of Medicine, Jeju National University School of Medicine) ;
  • Kang, Ji-Hoon (Department of Medicine, Jeju National University School of Medicine) ;
  • Koh, Young-Sang (Department of Medicine, Jeju National University School of Medicine) ;
  • Kim, Young-Heui (R&D Center, Bioland Ltd.) ;
  • Kim, Ki-Ho (R&D Center, Bioland Ltd.) ;
  • Ko, Ji-Hee (Department of Medicine, Jeju National University School of Medicine) ;
  • Yoo, Eun-Sook (Department of Medicine, Jeju National University School of Medicine) ;
  • Kang, Hee-Kyoung (Department of Medicine, Jeju National University School of Medicine)
  • 투고 : 2016.01.06
  • 심사 : 2016.11.15
  • 발행 : 2017.07.01
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초록

4-O-methylhonokiol, a neolignan compound from Magnolia Officinalis, has been reported to have various biological activities including hair growth promoting effect. However, although transforming growth factor-${\beta}$ (TGF-${\beta}$) signal pathway has an essential role in the regression induction of hair growth, the effect of 4-O-methylhonokiol on the TGF-${\beta}$ signal pathway has not yet been elucidated. We thus examined the effect of 4-O-methylhonokiol on TGF-${\beta}$-induced canonical and noncanonical pathways in HaCaT human keratinocytes. When HaCaT cells were pretreated with 4-O-methylhonokiol, TGF-${\beta}1$-induced G1/G0 phase arrest and TGF-${\beta}1$-induced p21 expression were decreased. Moreover, 4-O-methylhonokiol inhibited nuclear translocation of Smad2/3, Smad4 and Sp1 in TGF-${\beta}1$-induced canonical pathway. We observed that ERK phosphorylation by TGF-${\beta}1$ was significantly attenuated by treatment with 4-O-methylhonokiol. 4-O-methylhonokiol inhibited TGF-${\beta}1$-induced reactive oxygen species (ROS) production and reduced the increase of NADPH oxidase 4 (NOX4) mRNA level in TGF-${\beta}1$-induced noncanonical pathway. These results indicate that 4-O-methylhonokiol could inhibit TGF-${\beta}1$-induced cell cycle arrest through inhibition of canonical and noncanonical pathways in human keratinocyte HaCaT cell and that 4-O-methylhonokiol might have protective action on TGF-${\beta}1$-induced cell cycle arrest.

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참고문헌

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