분석과학 (Analytical Science and Technology)

  • 제30권3호
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  • Pages.130-137
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  • 2017
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  • 1225-0163(pISSN)
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  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
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DOI QR코드

DOI QR Code

Development of HPLC method for differentiation of three parts of mulberry tree

  • Eom, Ji Hyun (College of Pharmacy, Chungnam National University) ;
  • Vu, Thi Phuong Duyen (College of Pharmacy, Chungnam National University) ;
  • Cai, Linxi (College of Pharmacy, Chungnam National University) ;
  • Zhao, Yan (College of Pharmacy, Chungnam National University) ;
  • Li, Hong Xu (College of Pharmacy, Chungnam National University) ;
  • Yang, Seo Young (College of Pharmacy, Chungnam National University) ;
  • Kim, Young Ho (College of Pharmacy, Chungnam National University) ;
  • Kim, Seok Jin (MSC Annexed Food Technology Research Institute) ;
  • Cho, Hyun So (MSC Annexed Food Technology Research Institute) ;
  • Bao, Haiying (School of Chinese Medicinal Material, Jilin Agricultural University) ;
  • Chem, Jianbo (Chinese Academy of Agriculture Sciences) ;
  • Kim, Kyung Tae (College of Pharmacy, Chungnam National University) ;
  • Kang, Jong Seong (College of Pharmacy, Chungnam National University)
  • 투고 : 2017.06.01
  • 심사 : 2017.06.10
  • 발행 : 2017.06.25
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초록

The leaves (Mori Folium; MF), branches (Mori Ramulus; MR), and root bark (Mori Cortex Radicis; MCR) of the mulberry tree have been used as therapeutic herbs for centuries. Existing analytical methods were developed specifically for different parts of the tree and cannot be applied to samples containing a mixture of tree parts. Such method specialization is time-consuming and requires separate identification and quality control of each tree part. This report describes an HPLC method for the simultaneous quality control and discrimination of MF, MR, and MCR using four marker compounds: rutin, kuwanon G, oxyresveratrol, and morusin. An Optimapak $C_{18}$ column ($4.6{\times}250mm$, $5{\mu}m$) was used with a gradient elution of 0.1 % formic acid in water and acetonitrile. The flow rate was 1.0 mL/min and the detection wavelength was 270 nm. In quantitative analyses of the three parts, rutin (0.11 % w/w) was detected only in MF. The oxyresveratrol content (0.12 % w/w) was highest in MR. Kuwanon G (0.33 % w/w) and morusin (0.18 % w/w) were higher in MCR than in other parts. The HPLC method given herein can be used to simultaneously classify and quantify three herbal medicines from the mulberry tree.

키워드

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