Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
권호 표지
DOI QR코드

DOI QR Code

Biocidal Effects of Chlorine Dioxide on Isolated and Identified Pathogens from Nosocomial Environment - Biochemical and Technical Covergence

병원내 환경으로부터 분리 및 확인된 병원균에 대한 이산화염소의 살균 효과 - 생화학 및 기술 융합

  • Song, Kyoung-Ju (Purgofarm Co. Ltd) ;
  • Jung, Suk-Yul (Molecular Diagnostics Research Institute, Dept. of Biomedical Laboratory Science, Namseoul University)
  • 송경주 ((주)푸르고팜) ;
  • 정석률 (남서울대학교 임상병리학과 분자진단연구소)
  • Received : 2017.05.01
  • Accepted : 2017.06.20
  • Published : 2017.06.28
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, microorganisms were isolated from nosocomial environment and are identified by biochemical analysis as the part of biochemical and technical convergence. Microorganisms were collected at intense care unit of general hospital located in Pyeongtak (2014.11.28. - 2014. 11. 30). Using a VITEK2 equipment of biochemical approaches, eleven microorganisms e.g., Micrococcus luteus (or M. lylae), Granulicatella adiacens (M. luteus or M. lylae), Staphylococcus caprae, Sphingomonas paucimobilis, Kocuria kristinae, G elegans, Aerococcus viridans (or Staphylococcus arlettae), Methylobacterium spp., Dermacoccus nishinomiyaensis (or Kytococcus sedentarius), Kocuria kristinae (or M. luteus, M. lylae), Pseudomonas oryzihabitans were identified. And then identified bacteria plates were applied with a plastic stick, so called with "FarmeTok (medistick/Puristic) to produce ClO2. ClO2-releasing plastic stick showed the very strong inhibition of bacterial growth with about 99.9%. There were no bacterial colonies on the ClO2-incubated plate. Taken together, it is suggested that chlorine dioxide should be very strong inhibitor to microorganisms of nosocomial infections.

본 연구에서는, 생화학 및 기술학 융합 연구의 일환으로, 병원내 환경으로부터 미생물이 추출되고 생화학적 분석으로 통하여 동정되었다. 미생물은 평택 소재 평원 중환자실에서 수집되었다 (2014년 11월 28일부터 2014년 11월 30일). 생화학장비인 VITEK2를 이용하여 11개의 미생물, Micrococcus luteus (or M. lylae), Granulicatella adiacens (M. luteus or M. lylae), Staphylococcus caprae, Sphingomonas paucimobilis, Kocuria kristinae, G elegans, Aerococcus viridans (or Staphylococcus arlettae), Methylobacterium spp., Dermacoccus nishinomiyaensis (or Kytococcus sedentarius), Kocuria kristinae (or M. luteus, M. lylae), Pseudomonas oryzihabitans이 동정되었다. 이후 이산화염소가스를 배출하는 팜이톡이라고 하는 플라스틱 막대와 함께 두고 측정해본 결과, 약 99.9% 이상의 매우 강한 미생물 성장억제가 분석되었다. 팜이톡과 함께 배양된 미생물 플레이트에는 어떠한 세균 집락도 발견되지 않았다. 종합해볼 때, 이산화염소가스를 배출하는 팜이톡은 병원내 감염을 유발하는 미생물에 대한 매우 강한 성장 억제효과를 가지는 것으로 확인되었다.

Keywords

References

  1. H. Vogt, J. Balej, J. E. Bennett, P. Wintzer, S. A. Sheikh, P. Gallone, S. Vasudevan, K. Pelin, Chlorine oxides and chlorine oxygen acids, Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH, 2010.
  2. L. O. Brockway, "The three-electron bond in chlorine dioxide", Proc. Natl. Acad. Sci. USA, vol. 19, No. 3, pp. 303-307, 1933. https://doi.org/10.1073/pnas.19.3.303
  3. R. Flesch, J. Plenge, E. Ruhl, "Core-level excitation and fragmentation of chlorine dioxide", Int. J. Mass Spectrometry, Vol. 249-250, pp. 68-76, 2006. https://doi.org/10.1016/j.ijms.2005.12.046
  4. T. Sanekata, F. Fukuda, T. Miura, H. Morino, C. Lee, K. Maeda, K. Araki, T. Otake, T. Kawahata, T. Shibata, "Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus", Biocontrol. Science, Vol. 15, No. 2, pp. 45-49, 2010. https://doi.org/10.4265/bio.15.45
  5. N. Ogata, T. Shibata, "Protective effect of low-concentration chlorine dioxide gas against influenza a virus infection", J. Gen. Virology, Vol. 89, No. 1, pp. 60-67, 2008. https://doi.org/10.1099/vir.0.83393-0
  6. H. Morino, T. Fukuda, T. Miura, C. Lee, T. Shibata, T. Sanekata, "Inactivation of feline calicivirus, a norovirus surrogate, by chlorine dioxide gas", Biocontrol. Science, Vol. 14, No. 4, pp. 147-153, 2009. https://doi.org/10.4265/bio.14.147
  7. J. W. Ma, B. S. Huang, C. W. Hsu, C. W. Peng, M. L. Cheng, J. Y. Kao, T. D. Way, H. C. Yin, S. S. Wang, "Efficacy and Safety Evaluation of a Chlorine Dioxide Solution", Int. J. Environ. Res. Public Health, Vol. 14, No. 3, pp. 329, 2017. https://doi.org/10.3390/ijerph14030329
  8. I. Ofori, S. Maddila, J. Lin, S. B. Jonnalagadda, "Chlorine dioxide oxidation of Escherichia coli in water - A study of the disinfection kinetics and mechanism", J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. England, Vol. 1-9, 2017. [Epub ahead of print].
  9. J. H. Kang, K. B. Song, "Combined treatment on the inactivation of naturally existing bacteria and Escherichia coli O157:H7 inoculated on fresh-cut Kale", J. Microbiol. Biotechnology, Vol. 27, No. 2, pp. 219-225, 2017. https://doi.org/10.4014/jmb.1607.07029
  10. R. Nogueira, K. U. Utecht, M. Exner, W. Verstraete, K. H. Rosenwinkel. "Strategies for the reduction of Legionella in biological treatment systems", Water. Sci. Technology, Vol. 74, No. 4, pp. 816-823, 2016. https://doi.org/10.2166/wst.2016.258
  11. A. S. Breathnach, "Nosocomial infections", Medicine, Vol. 37, No. 10, pp. 557-561, 2009. https://doi.org/10.1016/j.mpmed.2009.07.008
  12. S. Y. Jeong, O. S. Kim, J. Y. Lee, "The status of Healthcare-associated Infection Control among Healthcare Facilities in Korea" J. Digital Policy & Management, Vol. 12, No. 5, pp. 353-366, 2014.
  13. S. W. Jeung, C. H. Lim, I. G. You, "A Study on the Measurement of Bacterial Contamination of MRI Examination Equipment and Disinfection Conditions", J. Digital Policy & Management, Vol. 11, No. 12, pp. 665-672, 2013.
  14. C. W. Seo, Y. B. Yu, K. S. Shin, Y. K. Kim, "Distribution of Yeast Isolated from Clinical Specimens at a University Hospital in last Five Years, J. Digital Policy & Management, Vol. 12, No. 9, pp. 237-244, 2014.
  15. P. K. Shin, "A convergence study the association between addictive smart phone use, dry eye syndrome, upper extremity pain and depression among college students', J. Korea Converg. Soc., Vol. 8, No. 1, pp. 61-69, 2017. https://doi.org/10.15207/JKCS.2017.8.1.061
  16. J. J. Lowe, S. G. Gibbs, P. C. Iwen, P. W. Smith, A. L. Hewlett, "Impact of Chlorine Dioxide Gas Sterilization on Nosocomial Organism Viability in a Hospital Room", Int. J. Environ. Res. Public Health, Vol. 10, No. 6, pp. 2596-2605, 2013. https://doi.org/10.3390/ijerph10062596
  17. K. S. Yao, Y. H. Hsieh, Y. J. Chang, C. Y. Chang, T. C. Cheng, H. L. Liao, "Inactivation effect of chlorine dioxide on phytophathogenic bacteria in irrigatin water", J. Environ. Eng. Management, Vol. 20, No. 3, pp. 157-160, 2010.
  18. X. W. Wang, J. S. Li, M. Jin, B. Zhen, Q. X. Kong, N. Song, W. J. Xiao, J. Yin, W. Wei, G. J. Wang, B. Y. Si, B. Z. Guo, C. Liu, G. R. Ou, M. N. Wang, T. Y. Fang, F. H. Chao, J. W. Li, "Study on the resistance of severe acute respiratory syndrome-associated coronavirus", J. Virol. Methods, Vol. 126, No. 1-2, pp. 171-177, 2005. https://doi.org/10.1016/j.jviromet.2005.02.005
  19. C. Chen, X. J. Zhang, Y. Wang, L. X. Zhu, J. Liu, "Waste water disinfection during SARS epidemic for microbiological and toxicological control", Biomed. Environ. Science, Vol. 19, No. 3, pp. 173-178, 2006.
  20. K. M. Yang, "The effect of self-esteemand depression on smartphone addiction among university students", J. Korea Converg. Soc., Vol. 7, No. 1, pp. 113-123, 2016. https://doi.org/10.15207/JKCS.2016.7.1.113
  21. J. L. Cui, M. H. Chey, S. I. Kim, "Seismic performance of urban structures with various horizontal irregularities using equivalent static analysis", J. IT Converg. Soc. SMB, Vol. 6, No. 1, pp. 25-32, 2016.
  22. H. J. Oh, H. Y. Doo, S. H. Sim, S. M. Choi, S. J. Oh, "The Study of tear film stability for normal eyes in adults", J. IT Converg. Soc. SMB, Vol. 6, No. 4, pp. 93-98, 2016. https://doi.org/10.22156/CS4SMB.2016.6.4.093