Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Development of a Method to Detect Cattle Material from Processed Meat Products Using a Polymerase Chain Reaction

PCR을 이용한 축산물 가공식품 내 소고기 성분 검출법 개발

  • Kwon, Young Chul (Department of Medical Laboratory Science, Jinju Health College) ;
  • Hah, Do-Yun (Gyeongnam Livestock Promotion Research Institute) ;
  • Heo, Yunwi (Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Tae-Kyu (Gyeongnam Livestock Promotion Research Institute) ;
  • Choi, Yoo-Jeong (Gyeongnam Livestock Promotion Research Institute) ;
  • Jo, Dae-Hoon (Gyeongnam Livestock Promotion Research Institute) ;
  • Nam, Sang-Yun (Gyeongnam Livestock Promotion Research Institute) ;
  • Son, Byeong-Guk (Gyeongnam Livestock Promotion Research Institute) ;
  • Hwang, Bo-Won (Gyeongnam Livestock Promotion Research Institute) ;
  • Yang, Byoung-Seon (Department of Medical Laboratory Science, Jinju Health College) ;
  • Kim, Euikyung (Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University)
  • 권영철 (진주보건대학교 임상병리과) ;
  • 하도윤 (경상남도 축산진흥연구소) ;
  • 허윤위 (경상대학교 수의과대학 약리.독성학교실) ;
  • 김태규 (경상남도 축산진흥연구소) ;
  • 최유정 (경상남도 축산진흥연구소) ;
  • 조대훈 (경상남도 축산진흥연구소) ;
  • 남상윤 (경상남도 축산진흥연구소) ;
  • 손병국 (경상남도 축산진흥연구소) ;
  • 황보원 (경상남도 축산진흥연구소) ;
  • 양병선 (진주보건대학교 임상병리과) ;
  • 김의경 (경상대학교 수의과대학 약리.독성학교실)
  • Received : 2016.11.29
  • Accepted : 2017.04.10
  • Published : 2017.06.30
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Abstract

Polymerase chain reaction (PCR) was used to detect cattle material from processed meat products. Seventy-eight different commercial processed meat products were purchased from several big food marts. Among them, 17 products contained cattle material (10 samples contained only cattle, 5 samples mixed with cattle and porcine, 2 samples mixed with cattle, porcine and chicken). The genomic DNA was extracted directly from the processed meat products, and strain-specific primer targeting the 16S ribosomal RNA mitochondrial gene was used. All PCR products were cloned into the pGEM-T easy vector and sequenced. Consequently, the PCR products were amplified from 10 processed meat products, which contained only cattle material in our conditions. Furthermore, PCR reactions showed the same results at mixed samples. The DNA sequence obtained from pGEM-T easy/PCR products showed more than 95% identity with Bos taurus 16S rRNA gene using homology analysis. In conclusion, we suggest that the method using PCR, as performed in this study, could be useful in detecting cattle material in processed meat products. Moreover, our system could be applicable in inspection procedures to improve the verification of correct labeling for import and export processed meat products.

중합효소연쇄반응법을 이용한 축산물 가공식품 내에 존재하는 소고기 성분을 특이적으로 검출할 수 있는 방법을 개발하기 위하여 축산물 가공식품 78종류를 무작위로 선별하였다. 가공식품으로부터 추출한 genomic DNA를 이용하여 소의 미토콘드리아 16S rRNA 염기서열을 이용하여 strain-specific primer를 직접 제작하여 중합효소연쇄반응을 수행한 후, 증폭된 반응산물의 염기서열을 분석 하였다. 축산물 가공식품 내 소고기 성분 검출을 위한 중합효소연쇄반응 수행 결과, 소고기 성분이 함유되어 있는 17개의 축산물 가공식품이 정확히 증폭되었고, 증폭산물의 DNA 염기서열 분석 결과 소의 미토콘드리아 16S rRNA 서열과 95% 이상의 상동성을 보였다. 본 실험에서 제시된 방법으로 축산물 가공식품 내 소고기 성분검출을 적용하였을 시, 소고기 성분이 함유된 축산물 가공식품을 정확하게 감별할 수 있었으며, 나아가 식품 원재료의 허위기재 등에 의한 불량식품 유통 근절 및 종교적 이유로 인한 금기 식품감별 등과 같은 과학적 식품 감시에 기여할 수 있다고 사료된다.

Keywords

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