한방비만학회지 (Journal of Korean Medicine for Obesity Research)

  • 제17권1호
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  • Pages.1-9
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  • 2017
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  • 1976-9334(pISSN)
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  • 2288-1522(eISSN)
한방비만학회 (The Society of Korean Medicine for obesity Research)
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Lipopolysaccharide를 처리한 RAW 264.7 세포에서 백두옹 메탄올 추출물의 항염증 효과

The Anti-Inflammatory Effect of Pulsatilla koreana Methanol Extract in Lipopolysaccharid-Exposed RAW 264.7 Cells

  • 이귀선 (상지대학교 한의과대학 한방재활의학교실) ;
  • 김두희 (상지대학교 한의과대학 한방재활의학교실) ;
  • 박중현 (상지대학교 한의과대학 한방재활의학교실) ;
  • 최희승 (상지대학교 한의과대학 한방재활의학교실) ;
  • 허성규 (상지대학교 한의과대학 한방재활의학교실) ;
  • 차윤엽 (상지대학교 한의과대학 한방재활의학교실)
  • Lee, Gui Sun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Kim, Doo Hee (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Park, Joong Hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Choi, Hee Seung (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Heo, Seong Kyu (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University) ;
  • Cha, Yun Yeop (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Sangji University)
  • 투고 : 2017.04.07
  • 심사 : 2017.05.30
  • 발행 : 2017.06.30
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초록

Objectives: This study was performed to identify the anti-inflammatory effect of Pulsatilla koreana (PK) methanol extract on lipopolysaccharide (LPS) induced inflammatory. Methods: There were five groups. They were control group, LPS-exposed PK methanol extract group ($0{\mu}g/ml$, $10{\mu}g/ml$, $30{\mu}g/ml$, $100{\mu}g/ml$). To measure out cytotoxicity of PK, we performed the MTT assay. To evaluate the anti-inflammatory effect of PK, we examined the inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines (interleukin $[IL]-1{\beta}$, IL-6, tumor necrosis $factor-{\alpha}$ [$TNF-{\alpha}$], IL-10). Results: 1. The extract of PK (${\sim}100{\mu}g/ml$) itself did not have any cytotoxic effect in RAW 264.7 cells. 2. The concentration of plasma NO and PGE2 in PK methanol extract group showed a lower values than those of control group. 3. The concentration of plasma $IL-1{\beta}$, plasma IL-6, and plasma $TNF-{\alpha}$ in PK methanol extract group showed a lower values than those of control group. 4. The concentration of Plasma IL-10 in PK methanol extract groups showed higher than control group; however, these values showed no significantly different. Conclusions: According to this study, the extract of PK could be used as a protective agent against inflammation.

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피인용 문헌

  1. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2017, https://doi.org/10.15429/jkomor.2020.20.2.149