The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Four Oriental Medicines on Secretion and Gene Expression of Mucin in Airway Epithelial Cells

가미청폐탕, 가감정기탕, 가미사물탕, 가미이중탕이 호흡기 뮤신의 분비 및 뮤신 유전자의 발현에 미치는 영향

  • Lee, Hyun Sook (Department of Pediatrics, College of Oriental Medicine, Dongguk University) ;
  • Min, Sang Yeon (Department of Pediatrics, College of Oriental Medicine, Dongguk University) ;
  • Kim, Jang Hyun (Department of Pediatrics, College of Oriental Medicine, Dongguk University)
  • 이현숙 (동국대학교 한의과대학 소아과학교실) ;
  • 민상연 (동국대학교 한의과대학 소아과학교실) ;
  • 김장현 (동국대학교 한의과대학 소아과학교실)
  • Received : 2017.02.10
  • Accepted : 2017.05.20
  • Published : 2017.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives In this study, the author intended to investigate whether Gami-cheongpetang (GCP), Gagam-jeongkitang (GJG), Gami-samooltang (GSM) and Gami-ijoongtang (GIJ) significantly affect in vivo (animal model) and in vitro (cultured cells) mucin secretion and MUC5AC gene expression in airway epithelial cells. Methods For in vivo experiment, the author induced hypersecretion of airway mucin in rats by introducing SO2 for 3 weeks. Enzyme-linked immunosorbent assay (ELISA) was used to assess the effects of orally-administered GCP, GJG, GSM and GIJ in vivo mucin secretion from tracheal goblet cells of rats after 1 week. Also, the effects of the agents on TNF- or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agents were assessed by examining the rate of survival and proliferation of NCI-H292 cells. Results (1) GCP and GJG significantly inhibited hypersecretion of in vivo mucin, although GSM and GIJ did not affect hypersecretion of in vivo mucin; (2) GCP and GJG significantly increased in vitro mucin secretion from cultured HTSE cells. However, GSM and GIJ did not affect in vitro mucin secretion from HTSE cells; (3) GCP and GJG significantly inhibited the expression levels of EGF-induced MUC5AC gene in NCI-H292 cells. However, GSM and GIJ increased the expression levels of EGF-induced MUC 5AC gene in NCI-H292 cells; (4) GCP, GJG, GSM and GIJ did not significantly inhibit the survival and proliferation of NCI-H292 cells. Conclusions These results suggest that GCP, GJG, GSM and GIJ can not only affect the secretion of mucin but also affect the expression of mucin gene. The author suggests that the effects of GCP, GJG, GSM and GIJ with their components should be further investigated by using animal experimental models that simulate the diverse pathophysiology of pulmonary diseases.

Keywords

References

  1. Jeon E. Soayakjeungjikgyeol. Seoul: Yeogangchulpansa. 2002:35-6.
  2. Oh GT. Onbyeongjobyeon. Seoul: Jipmoondang. 2001:711.
  3. Kim KB, Kim DG, Kim YH, Kim JH, Min SY, Park EJ, Beak JH, Yoo SE, Lee SY, Lee JY, Lee HJ, Jang GT, Chae JW, Han YJ, Han JK. Hanbangsoeacheongsoenyeoneuihak (sang). Seoul: Euisungdang. 2015:32.
  4. Joe MJ. Mechanism of mucin release from primary cultured hamster tracheal surface epithelial cells and characterization of a novel mucin-associated protein. Graduate school of Seoul National University. 2000.
  5. Jeongukhanuigwadaehak byeongrihakgyosil. Donguipegyenaegwahak. Seoul: Hanmunhwasa. 2002:102-14.
  6. Culpitt SV, Rogers DF, Traves SL, Barner PJ, Donnelly LE. Sputum matrix metalloproteases: comparison between chronic obstructive pulmonary disease and asthma. Respir Med. 2005;99(6):703-10. https://doi.org/10.1016/j.rmed.2004.10.022
  7. Rogers DF. Airway mucus hypersecretion in asthma: an undervalued pathology?. Curr Opin Pharmacol. 2004;4(3):241-50. https://doi.org/10.1016/j.coph.2004.01.011
  8. Korean academy of pediatric allergy & respiratory disease. Pediatric allergic & respiratory disease. Seoul: Gunjachulpansa. 2005:350-4.
  9. Rogers DF, Barnes PJ. Treatment of airway mucus hypersecretion. Ann Med. 2006;38(2):116-25. https://doi.org/10.1080/07853890600585795
  10. Rogers DF. Mucociliary dysfunction in COPD: effect of current pharmaco-therapeutic options. Pulm Pharmacol Ther. 2005;18(1):1-8. https://doi.org/10.1016/j.pupt.2004.08.001
  11. Shanghai University of Traditional Chinese Medicine. Junguinaeguahak. Hongkong: Commer Prss. 1982:24-5.
  12. Lee CJ. Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides. J Appl Pharmacol. 2001;9(3):218-23.
  13. Ko KH, Lee CJ, Shin CY, Jo MJ, Kim KC. Inhibition of mucin release from airway goblet cells by polycationic peptides. Am J Physiol. 1999;(21):L811-5.
  14. Lee CJ. Polycationic action mechanism for the inhibition of mucin release from trachea of rodents. Graduate school of Seoul National University. 1997.
  15. Pon DJ, Van Staden CJ, Boulet L, Rodger IW. Hyperplastic effects of aerosolized sodium metabisulfite on rat airway mucus-secretory epithelial cells. Can J Physiol Pharmacol. 1994;72:1025-30. https://doi.org/10.1139/y94-143
  16. Harkema JR, Hotchkiss JA. In vivo effects of endotoxin on intraepithelial mucosubstances in rat pulmonary airways: quantitative histochemistry. Am J Pathol. 1992; 141:307-31.
  17. St George JA, Cranz DL, Zicker SC, Etchison JR, Dungworth DL, Plopper CG. An immunohistochemical characterization of rhesus monkey respiratory secretions using monoclonal antibodies. Am Rev Respir Dis. 1985;132:556-63.
  18. Kim KC. Possible requirement of collagen gel substratum for production of mucin-like glycoproteins by primary rabbit tracheal epithelial cells in culture. In Vitro Cell Dev Biol. 1985;1:617-21.
  19. Kim KC, Rearick JI, Nettesheim P, Jetten AM. Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J Biol Chem. 1985;260:4021-7.
  20. Kim KC, Brody JS. Gel contraction causes mucin release in primary hamster tracheal epithelial cells growing on a collagen gel. J Cell Biol. 1987;105:158a.
  21. Wu R, Smith D. Continuous multiplication of rabbit tracheal epithelial cells in a defined, hormone-supplemented medium. In Vitro. 1982;18:800-12. https://doi.org/10.1007/BF02796504
  22. Wu R, Nolan E, Turner C. Expression of tracheal differentiated function in serum-free hormone-supplemented medium. J Cell Physiol. 1985;125:167-81. https://doi.org/10.1002/jcp.1041250202
  23. Karlinsey J, Stamatoyannopoulos G, Enver T. Simultaneous purification of DNA and RNA from small numbers of eukaryotic cells. Anal Biochem. 1989;180(2):303-6. https://doi.org/10.1016/0003-2697(89)90435-1
  24. Kim YD, Kwon EJ, Park DW, Song SY, Yoon SK, Baek SH. Interleukin-1beta induces MUC2 and MUC5AC synthesis through cyclooxygenase-2 in NCI-H292 cells. Mol Pharmacol. 2002;62(5):1112-8. https://doi.org/10.1124/mol.62.5.1112
  25. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer drug screening. J Natl Cancer Inst. 1990;82:1107-12. https://doi.org/10.1093/jnci/82.13.1107
  26. Lee BG, Kim TH, Park YB. Hanbangjindanhak (II). Seoul: Seungbosa. 2000:57.
  27. Kim WH. Principle of Korean Medicine. Seoul: Seungbosa. 2003:129-32, 165-8, 352-8.
  28. Lung system-Internal medicine of the national Korean oriental medical college. Donguipyegyenaeguahak. Seoul: Hanmoonhwasa. 2002:52-5, 102-14, 144-99.
  29. Kim YH, Han JG. Effects of Chwi-yeon-tang and Chihyosan-gamibang on airway mucus secretion and contractility of tracheal smooth muscle. J Korean Orient Pediatr. 2005;19(1):1-26.
  30. Hong CE. Pediatrics. Seoul: Daehangyogwaseo. 2005: 655-74.
  31. The Korean academy of asthma, allergy and clinical immunology. Asthma and allergy. Seoul: Gunjachulpansa. 2002:73-6.
  32. Kim YH. Cheongganguigam. Seoul: Seungbosa. 1988: 84-5, 102.
  33. Chae HY, Han JG, Kim YH. Effects of Gagam-jeonggitang, Gami-hwajeongjeon and Gami-tonggyutang on secretion of airway mucus in vitro and in vivo. J Korean Orient Pediatr. 2007;21(1):117-37.
  34. Lee JH. Doseolhanbanghaeseol. Seoul: Seungbosa. 1996: 386-7.
  35. Shin JY. Bangyakhappyeunhaeseol. Seoul: Seungbosa. 1991:23.