DOI QR코드

DOI QR Code

Antimicrobial efficacy and safety analysis of zinc oxide nanoparticles against water borne pathogens

  • Supraja, Nookala (Nanotechnology laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University) ;
  • Avinash, B. (Department of Veterinary Parasitology, College of Veterinary science, Sri Venkateswara Veterinary University) ;
  • Prasad, T.N.V.K.V. (Nanotechnology laboratory, Institute of Frontier Technology, Regional Agricultural Research Station, Acharya N G Ranga Agricultural University)
  • 투고 : 2016.10.25
  • 심사 : 2017.02.28
  • 발행 : 2017.06.25

초록

Metal nanoparticles have been intensively studied within the past decade. Nano-sized materials have been an important subject in basic and applied sciences. Zinc oxide nanoparticles have received considerable attention due to their unique antibacterial, antifungal, and UV filtering properties, high catalytic and photochemical activity. In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. In the emerging issue of increased multi-resistant properties in water borne pathogens, zinc oxide (ZnO) nanoparticle are being used increasingly as antimicrobial agents. Thus, the minimum bactericidal concentration (MBC) and minimum fungal concentration of ZnO nanoparticles towards pathogens microbe were examined in this study. The results obtained suggested that ZnO nanoparticles exhibit a good anti fungal activity than bactericidal effect towards all pathogens tested in in-vitro disc diffusion method (170 ppm, 100 ppm and 30 ppm). ZnO nanoparticles can be a potential antimicrobial agent due to its low cost of production and high effectiveness in antimicrobial properties, which may find wide applications in various industries to address safety issues. Stable ZnO nanoparticles were prepared and their shape and size distribution characterized by Dynamic light scattering (35.7 nm) and transmission electron microscopic TEM study for morphology identification (20 nm), UV-visible spectroscopy (230 nm), X-ray diffraction (FWHM of more intense peak corresponding to 101 planes located at $36.33^{\circ}$ using Scherrer's formula), FT-IR (Amines, Alcohols, Carbonyl and Nitrate ions), Zeta potential (-28.8). The antimicrobial activity of ZnO nanoparticles was investigated against Bacteria and Fungi present in drinking water PVC pipelines biofilm. In these tests, Muller Hinton agar plates were used and ZnO nanoparticles of various concentrations were supplemented in solid medium.

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참고문헌

  1. Altman, H., Steinberg, D., Porat, Y., Mor, A., Fridman, D. and Friedman, M. (2006), "In vitro assessment of antimicrobial peptides", J. Antimicrob. Chemother., 58(1), 198-201. https://doi.org/10.1093/jac/dkl181
  2. Asai, T., Kojima, A., Harada, K., Ishihara, K., Takahashi, T. and Tamura, Y. (2005), "Correlation between the usage volume of veterinary therapeutic antimicrobials and resistance in Escherichia coli isolated from the feces of food-producing animals in Japan", J. Infect. Disease., 58(6), 369-372.
  3. Baxter, J.B. and Aydil, E.S. (2005), "Nanowire based dye sensitized solar cells", Appl. Phys. Lett., 86(5), 53114-53119. https://doi.org/10.1063/1.1861510
  4. Bayandori Moghaddam, A., Kazemzad, M., Nabid, M.R. and Dabaghi, H.H. (2008), "Preparation of polyaniline/nanometer-scale alumina composite by the potential cycling method", Int. J. Electrochem. Sci., 3, 768-776.
  5. Brayner, R., Ferrari-lliou, R., Brivois, N., Djediat, S., Benedetti, M.F. and Fievet, F. (2006), "Toxicological effect of ZnO nanoparticles based on bacteria", Langmuir, 24(8), 4140-4144. https://doi.org/10.1021/la7035949
  6. Concannon, S.P., Crowe, T.D., Abercrombie, J.J., Molina, C.M., Hou, P. and Sukumaran, D.K. (2003), "Susceptibility of oral bacteria to an antimicrobial decapeptide", J. Med. Microbiol., 52(12), 1083-1093. https://doi.org/10.1099/jmm.0.05286-0
  7. Cullity, B.D. (1967), Elements of X-Ray Diffraction, (3re Edition ), Addison-Wesley, Reading, Mass, USA.
  8. Cynthia, H. and Callaghan, O. (1983), "Assessment of a new antibiotic", In: (W.B. Hugo, A.D. Russel, Editors), Pharmaceutical Microbiology 3, Blackwell Scientific Publications, Oxford, UK, pp. 122-134.
  9. Daglia, M., Papetti, A., Grisoli, P., Aceti, C., Dacarro, C. and Gazzani, G. (2007), "Antibacterial activity of red and white wine against oral streptococci", J. Agric. Food Chem., 55(13), 5038-5042. https://doi.org/10.1021/jf070352q
  10. Donaldson, K., Aitken, R., Tran, L., Stone, V., Duffin, R., Forrest, G. and Alexander, A. (2006), "Carbon nanotubes: a review of their properties in relation to pulmonary toxicology and workplace safety", Toxicol. Sci., 92(1), 5-22. https://doi.org/10.1093/toxsci/kfj130
  11. He, Y.T., Wan, J.M. and Tokunaga, T. (2008), "Kinetic stability of hematite nanoparticles the effect of particle sizes", J. Nanoparticle Res., 10(2), 321-332. https://doi.org/10.1007/s11051-007-9255-1
  12. Huang, M.H., Mao, S., Feick, H., Yan, H.Q., Wu, Y., Kind, H., Weber, E., Russo, R. and Yang, P. (2001), "ZnO microrods photodeposited with Au-Ag nanoparticles: Synthesis, characterization and application", In Sers. Sci., 292, 1897-1903.
  13. Jain, D., Kumar Daima, H., Kachhwaha, S. and Kothari, S.L. (2009), "Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their antimicrobial activities", A. Digest J. Nanomater. Biostruct., 4(3), 557-563.
  14. Jarvinen, H., Tenovuo, J. and Huovinen, P. (1993), "In vitro susceptibility of Streptococcus mutans to chlorhexidine and six other antimicrobial agents", Antimicrob Agents Chemother., 37(5), 1158-1159. https://doi.org/10.1128/AAC.37.5.1158
  15. Khoshhesab, Z.M., Sarfaraz, M. and Asadabad, M.A. (2011), "Preparation of ZnO nanostructures by chemical precipitation method", Synth. React. Inorganic Metal-Organ. Nano-Metal Chem., 41(7), 814-819. https://doi.org/10.1080/15533174.2011.591308
  16. Kim, Y.S., Seo, J.H. and Cha, H.J. (2003), "Enhancement of heterologous protein expression in Escherichia coli by co-expression of nonspecific DNA-binding stress protein, Dps. Enzyme", Microb. Technol., 33(4), 460-465. https://doi.org/10.1016/S0141-0229(03)00148-0
  17. Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y.H., Hwang, C.Y., Kim, Y.K., Lee, Y.S., Jeong, D.H. and Cho, M.H. (2007), "Antimicrobial effects of silver nanoparticles", J. Nanomed., 3(1), 95-101. https://doi.org/10.1016/j.nano.2006.12.001
  18. Kirchner, C., Liedl, T., Kudera, S., Pellegrino, T., Javier, A.M., Gaub, H.E., Stolzle, S., Fertig, N. and Parak, W.J. (2005), "Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles", Nano Lett., 5(2), 331-338. https://doi.org/10.1021/nl047996m
  19. Olowa, L.F. and Nuneza, O.M. (2013), "Brine shrimp lethality assay of the ethanolic extracts of three selected species of medicinal plants from Iligan City", Philippines Int. Res. J. Biol. Sci., 2(11), 74-77.
  20. Lu, S., Duffin, R., Poland, C., Daly, P., Murphy, F., Drost, E., MacNee, M., Stone, V. and Donaldson, K. (2009), "Efficacy of simple short-term in vitro assays for predicting the potential of metal oxide nanoparticles to cause pulmonary inflammation", Environ. Health Perspect., 117(2), 241-247. https://doi.org/10.1289/ehp.11811
  21. Nagarajan, P. and Rajagopalan, V. (2008), "Enhanced bioactivity of ZnO nano-particles-an antimicrobial study", Environ. Sci. Technol., 9(035004), 7-15.
  22. Olofsson, S.K. (2006), "Relation between drug exposure and selection of antibiotic resistant bacteria", Dissertation; Uppsala University, Faculty of Medicine, Uppsala, Sweden.
  23. Phillips, I., Casewe, M., Cox, T., Groot, D.B., Friis, C., Jones, R., Nightingale, C., Preston, R. and Waddell, J. (2004), "Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data", J. Antimicrobial Chemotherapy, 53(1), 28-52. https://doi.org/10.1093/jac/dkg483
  24. Reeves, D.S. (1999), Clinical Antimicrobial Assay, Oxford University Press, New York, USA.
  25. Rezaei-Zarchi, S., Imani, S., mohammad Zand, A., Saadati, M. and Zaghari, Z. (2012), "Study of bactericidal properties of carbohydrate-stabilized platinum oxide nanoparticles", J. Int. Nanolett., 5(2), 83-89.
  26. Sawai, J. and Yoshikawa, T. (2004), "Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay", J. Appl. Microbiol., 96(4), 803-808. https://doi.org/10.1111/j.1365-2672.2004.02234.x
  27. Sawai, J., Igarashi, H., Hashimoto, A., Kokugan, C.T. and Shimizu, M. (1996), "Antibacterial characteristics of magnesium oxide powder", J. Chem. Eng. Japan., 16(2), 187-194.
  28. Sawai, J., Doi, R., Maekawa, Y., Yoshikawa, T. and Kojima, H. (2002), "Short communication indirect conductimetric assay of antibacterial activities", J. Ind. Microbiol. Biotech., 29(5), 296-305. https://doi.org/10.1038/sj.jim.7000314
  29. Song, J., Zhou, J. and Wang, Z.L. (2006), "Piezoelectric and semi conducting coupled power generating process of a single ZnO belt/wire. A technology for harvesting electricity from the environment", Nano Lett., 6(8), 1656-1662. https://doi.org/10.1021/nl060820v
  30. Stoimenov, P.K., Klinger, R.L., Marchin, G.L. and Klabunde, K.J. (2002), "Metal oxide nanoparticles as bactericidal agents", Langmuir, 18(17), 6679-6685. https://doi.org/10.1021/la0202374
  31. Supraja, N., Prasad, T.N.V.K.V., Giridhara Krishna, T. and David, E. (2015), "Synthesis, characterization, and evaluation of the antimicrobial efficacy of Boswellia ovalifoliolata stem bark-extract-mediated zinc oxide nanoparticles", Appl. Nanosci., 6(4), 581-590. DOI: 10.1007/s13204-015-0472-0
  32. Tom, R.T., Suryanarayanan, V., Ganapati Reddy, P., Baskaran, S. and Pradeep, T. (2004), "Ciprofloxacinprotected gold nanoparticles", Langmuir, 20(5), 1909-1914. https://doi.org/10.1021/la0358567
  33. Wang, Z.L. (2004), "Functional oxides nanobelts materials, properties and potential applications in nanosystems and biotechnology", Annu. Rev. Phys. Chem., 55(159), 1656-1662.
  34. West, J.L. and Halas, N.J. (2000), "Applications of nanotechnology to biotechnology", Curr. Opin. Biotech., 11(2), 215-221. https://doi.org/10.1016/S0958-1669(00)00082-3
  35. Yamac, M. and Bilgili, F. (2006), "Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates", Pharm Biol., 44(9), 660-667. https://doi.org/10.1080/13880200601006897
  36. Zandonella, C. (2003), "Cell nanotechnology the tiny toolkit", Nature, 423(6935), 10-12. https://doi.org/10.1038/423010a