Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Volatile Compounds and Antiproliferative Effects of Dendropanax morbifera on HepG2 Cells

황칠나무의 휘발성 화합물 분석 및 HepG2 세포의 증식 억제 효과

  • Received : 2017.03.16
  • Accepted : 2017.04.27
  • Published : 2017.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Dendropanax morbifera Lev. is known in Korea for its golden sap and medicinal properties. The many biological activities of the leaf and stem extracts suggest that this tree could be a valuable source of medicinal compounds for the treatment of various ailments such as dermatitis, migraines, dysmenorrhea, muscle pain, and infectious diseases. However, there is little information on the composition and biological activity of the volatile fraction of D. morbifera. Therefore, in this study, the volatile compounds in leaves, stems, and sap of D. morbifera were isolated using solvent and supercritical fluid extraction (SFE), and analyzed by gas chromatography/mass spectrometry to reveal their chemical composition and identify potential compounds of interest. Fifteen compounds were identified in the leaf extracts, whereas 29 and 3 compounds were identified in the stem and sap extracts, respectively. The volatile profiles obtained using solvent and SFE differed. Esters and aromatic hydrocarbons predominated in the solvent extract of leaves and SFE extract of stems, whereas the solvent extract of stems and SFE extract of leaves contained terpenoids. Limonene, ${\alpha}$-pinene, and ${\beta}$-myrcene were identified in the volatile extract of sap, with limonene representing 96.30% of the total peak area. In addition, the antiproliferative effects of the solvent extracts of leaves and stems were evaluated, revealing that these solvent extracts were particularly effective in decreasing the proliferation of HepG2 cells.

황칠나무(Dendropanax morbifera Lev.)는 황금빛의 수액과 약리효과로 한국에서 알려져 있으며, 잎 및 줄기 추출물의 다양한 효능은 피부질환, 편두통, 월경통, 근육통 및 전염성 질환 등의 질병을 개선하는 약리 성분을 공급하는 우수한 공급원이 될 수 있는 것을 시사한다. 그러나, 황칠나무 추출물의 효능에 관해서는 다양한 연구가 보고되어 있으나, 부위별 휘발성 성분의 조성에 대한 보고는 전무한 상황이다. 따라서, 본 연구는 황칠나무의 잎, 줄기 및 수액의 주요 휘발성 성분을 규명하기 위하여, 유기용매 및 초임계유체추출법을 이용한 추출물을 가스크로마토그래피-질량분석법으로 분석하였다. 잎 추출물에서는 15가지 화합물이 검출되었으며, 줄기 및 수액에는 각각 29가지 및 3가지 성분이 확인되었다. 또한 용매와 초임계유체추출법을 사용하여 얻은 휘발성 성분의 프로파일은 다르게 나타났다. 잎의 용매 추출물과 줄기의 초임계유체 추출물에서는 에스테르와 방향족 탄화수소가, 잎의 초임계유체 추출물 및 줄기의 초임계유체 추출물에는 테르페노이드가 주요 성분으로 나타났다. 한편, limonene (96.3%), ${\alpha}$-pinene, 그리고 ${\beta}$-myrcene은 수액 용매 추출물의 휘발성 성분으로 확인되었다. 잎 및 줄기의 용매 추출물의 암세포 증식 억제 효과를 평가한 결과, 잎의 용매 추출물이 HepG2 세포의 증식을 유의적으로 감소시키는 것으로 나타났다.

Keywords

References

  1. Acree, T. and Arn, H. Flavornet and human odor space. Available from: http://www.flavornet.org. Accessed Nov. 15, 2015.
  2. Ahn, J., Kim, S., Kim, M., Kim, O., Kim, K. and Hwang, B. 2003. Seasonal variations in yields of Hwangchil lacquer and major sesquiterpene compounds from selected superior individuals of Dendropanax morbifera Lev. J. Plant Biol. 46, 38-40. https://doi.org/10.1007/BF03030299
  3. Bae, D., Kim, J., Lee, S. Y., Choi, E. J., Jung, M. A., Jeong, C., Na, J. R., Kim, J. J. and Kim, S. 2015. Hepatoprotective effects of aqueous extracts from leaves of Dendropanax morbifera Leveille against alcohol-induced hepatotoxicity in rats and in vitro anti-oxidant effects. Food Sci. Biotechnol. 24, 1495-1503. https://doi.org/10.1007/s10068-015-0193-x
  4. Chung, I. M., Kim. M. Y., Park, W. H. ans Moon, H. I. 2009. Antiatherogenic activity of Dendropanax morbifera essential oil in rats. Pharmazie 64, 547-549.
  5. Chung, I. M., Seo, S. H., Kang, E. Y., Park, S. D., Park, W. H. and Moon, H. I. 2009. Chemical composition and larvicidal effects of essential oil of Dendropanax morbifera against Aedes aegypti L. Biochem. Syst. Ecol. 37, 470-473. https://doi.org/10.1016/j.bse.2009.06.004
  6. Chung, I. M., Song, H. K., Kim, S. J. and Moon, H. I. 2011. Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille. Phytother. Res. 25, 784-786. https://doi.org/10.1002/ptr.3336
  7. Crowell, P. L., Chang, R. R., Ren, Z. B., Elson, C. E. and Gould, M. N. 1991. Selective inhibition of isoprenylation of 21-26-kDa proteins by the anticarcinogen d-limonene and its metabolites. J. Biol. Chem. 266, 17679-17685.
  8. Deepa, H. R. and Nalini, M. S. 2013. Phytochemical screening, total phenolic content and in vitro antioxidant studies of leaf, bark and flower extracts of Schefflera spp. (Araliaceae) J. App. Pharm. Sci. 3, 94-98.
  9. Duran-Pena, M. J., Botubol Ares, J. M., Hanson, J. R., Collado, I. G. and Hernandez-Galan, R. 2015. Biological activity of natural sesquiterpenoids containing a gem-dimethylcyclopropane unit. Nat. Prod. Rep. 32, 1236-1248. https://doi.org/10.1039/C5NP00024F
  10. Hyun, T. K., Kim, M. O., Lee, H., Kim, Y., Kim, E. and Kim, J. S. Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Leveille. Food Chem. 141, 1947-1955.
  11. Hyun, T. K., Ko, Y. J., Kim, E. H., Chung, I. M. and Kim, J. S. 2015. Anti-inflammatory activity and phenolic composition of Dendropanax morbifera leaf extracts. Ind. Crops Prod. 74, 263-270. https://doi.org/10.1016/j.indcrop.2015.05.002
  12. Kim, H. and Song, M. J. 2013. Oral traditional plant-based therapeutic applications for pain relief recorded in North Jeolla province, Korea. Indian J. Tradit. Know. 12, 573-584.
  13. Kim, S. H., Jang, Y. S., Han, J. G., Chung, H. G., Lee, S. W. and Cho, K. J. 2006. Genetic variation and population structure of Dendropanax morbifera Lev. (Araliaceae) in Korea. Silvae Genet. 55, 7-13. https://doi.org/10.1515/sg-2006-0002
  14. Lee, S. H., Lee, H. S., Park, Y. S., Hwang, B., Kim, J. H. and Lee, H. Y. 2002. Screening of immune activation activities in the leaves of Dendropanax morbifera Lev. Kor. J. Med. Crop Sci. 10, 109-115.
  15. Lima, C. F., Andrade, P. B., Seabra, R. M., Fernandes- Ferreira, M. and Pereira-Wilson, C. 2005. The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. J. Ethnopharmacol. 97, 383-389. https://doi.org/10.1016/j.jep.2004.11.029
  16. Lu, J. J., Dang, Y. Y., Huang, M., Xu, W. S., Chen, X. P. and Wang, Y. T. 2012. Anti-cancer properties of terpenoids isolated from Rhizoma Curcumae-A review. J. Ethnopharmacol. 143, 406-411. https://doi.org/10.1016/j.jep.2012.07.009
  17. Moon, H. I. 2011. Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats. Hum. Exp. Toxicol. 30, 870-875. https://doi.org/10.1177/0960327110382131
  18. Park, B. Y., Min, B. S., Oh, S. R., Kim, J. H., Kim, T. J., Kim, D. H., Bae, K. H. and Lee, H. K. 2004. Isolation and anticomplement activity of compounds from Dendropanax morbifera. J. Ethnopharmacol. 90, 403-408. https://doi.org/10.1016/j.jep.2003.11.002
  19. Yoo, K. Y. and Park, S. Y. 2012. Terpenoids as potential anti- Alzheimer's disease therapeutics. Molecules 17, 3524-2538. https://doi.org/10.3390/molecules17033524
  20. Yu, H. Y., Kim, K. S., Lee, Y. C., Moon, H. I. and Lee, J. H. 2012. Oleifolioside A, a new active compound, attenuates LPS-stimulated iNOS and COX-2 expression through the downregulation of $NF-{\kappa}b$ and MAPK activities in RAW 264.7 macrophages. Evid. Based Complement. Alternat. Med. 2012, 637512.
  21. Van Vuuren, S. F. and Viljoen, A. M. 2007. Antimicrobial activity of limonene enantiomers and 1,8-cineole alone and in combination. Flavour Frag. J. 22, 540-544. https://doi.org/10.1002/ffj.1843
  22. Wang, G. W., Qin, J. J., Cheng, X., Shen, Y. H., Shan, L., Jin, H. Z. and Zhang, W. D. 2014. Inula sesquiterpenoids: structural diversity, cytotoxicity and anti-tumor activity. Expert Opin. Inv. Drug. 23, 317-345. https://doi.org/10.1517/13543784.2014.868882