Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Fermented Garlic Extract on Adhesion of THP-1 Cells to Bovine Aortic Endothelial Cells

혈관내피세포에 부착하는 THP-1에 대한 발효마늘추출물의 효과

  • Kwon, Ohseong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University) ;
  • Song, Jina (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University) ;
  • Yu, Heui Jong (Health Food R&D Center) ;
  • Park, Heonyong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, Dankook University)
  • 권오성 (단국대학교 분자생물학과 / 켐바이오 글로벌 전문인력양성 사업단 / 나노센서바이오텍연구소) ;
  • 송지나 (단국대학교 분자생물학과 / 켐바이오 글로벌 전문인력양성 사업단 / 나노센서바이오텍연구소) ;
  • 유휘종 ((주)바이오랜드 식품연구소) ;
  • 박헌용 (단국대학교 분자생물학과 / 켐바이오 글로벌 전문인력양성 사업단 / 나노센서바이오텍연구소)
  • Received : 2016.12.23
  • Accepted : 2017.03.09
  • Published : 2017.05.30
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Abstract

Fermented garlic extract (FGE) is a well-known functional food containing cycloalliin. Here we tested if FGE has an effect on the vascular system. We found that FGE had an effect on monocytic adhesion to endothelial cells, which occurs at the initial step of processes of inflammation. From dose-dependent experiments, 0.1 to $1{\mu}g/ml$ of FGE appeared to inhibit lipopolysaccharide (LPS)-enhanced heterotypic cell adhesion between THP-1 and BAECs. This result indicates that FGE blocks vascular inflammation. Then we found that FGE activates eNOS and Akt in BAECs. The phosphorylation of eNOS was maximally elevated 10 min after FGE treatment. Parallely, the phosphorylation of Akt was also maximally increased 10 min after FGE treatment. Consistently, it was found that FGE enhanced the production of nitric oxide. We then examined whether NO mediates THP-1 cell adhesion to BAECs. Both Akt and eNOS inhibitors appeared to reverse an inhibitory effect of FGE. These findings indicate that FGE inhibits LPS-enhanced heterotypic cell adhesion via Akt and eNOS. In conclusion, FGE plays an important role in prevention of inflammatory diseases.

마늘은 전통적으로 cycloalliin을 포함한 기능성 식품으로 널리 알려져 있다. 본 연구를 통해 혈관계에서 발효마늘추출물이 어떤 효능을 갖는지 알아보았다. 먼저, 발효마늘추출물이 내피세포의 단핵구 부착을 억제하는 것을 관찰하였다. 농도 변화에 따른 실험결과, 발효마늘추출물을 0.1 또는 $1{\mu}g/ml$ 농도로 전처리 하면, LPS에 의하여 촉발되는 소 대동맥 내피세포에 달라붙는 THP-1의 부착이 억제되는 것을 확인하였다. 단핵구의 내피세포 부착이 염증반응 초기단계에 진행되는 과학적 근거를 고려하면 이와 같은 결과는 발효마늘추출물이 혈관 염증을 저해한다고 볼 수 있다. 또한 세포신호전달물질의 활성변화를 관찰한 결과, 발효마늘추출물이 소 대동맥 내피세포에서 eNOS와 Akt의 활성을 유도함을 알았다. eNOS의 인산화 반응은 발효마늘추출물을 처리한지 10분 후에 최대로 상승되었으며, 이와 유사하게 Akt도 발효마늘추출물을 처리하고 10분 후에서 인산화 반응이 최대로 상승되었다. 산화질소 양 변화 측정에서도 발효마늘추출물은 산화질소의 생성을 상승시켰다. 한편, 산화질소 생성을 저해하는 억제제 처리는 발효마늘추출물에 의해 나타나는 세포부착 저해능력을 감소시켰다. eNOS와 Akt를 매개로 하는 LPS 유도 세포 부착 정도가 발효마늘추출물에 의하여 억제된다는 것은 산화질소가 세포부착에 중요 매개물임을 의미한다. 결론적으로, 발효마늘추출물은 염증관련 질환을 예방하는 데에 중요한 역할을 한다고 할 수 있다.

Keywords

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