Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of dexamethasone on progesterone and estrogen profiles and uterine progesterone receptor localization during pregnancy in Sahel goat in Semi-Arid region

  • Yahi, Dauda (Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri) ;
  • Ojo, Nicholas Adetayo (Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri) ;
  • Mshelia, Gideon Dauda (Department of Theriogenology, Faculty of Veterinary Medicine, University of Maiduguri)
  • Received : 2017.03.01
  • Accepted : 2017.05.19
  • Published : 2017.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Despite the widespread use of dexamethasone in veterinary and human medicine, it is reported to cause some severe pregnancy related side effects like abortion in some animals. The mechanism of the response is not clear but seems to be related to interspecies and/or breed difference in response which may involve alterations in the concentrations of some reproductive hormones. Methods: Twenty Sahel goats comprising 18 does and 2 bucks were used for this study. Pregnancies were achieved by natural mating after synchronization. Repeated dexamethasone injections were given at 0.25 mg/kg body weight. Blood samples were collected biweekly for hormonal assay. Uterine biopsies were harvested at days 28 and day 78 of gestation through caesarean section for immunohistochemical analysis using 3 pregnant does randomly selected from each group at each instant. Data were expressed as Means ${\pm}$ Standard Deviations and analyzed using statistical soft ware package, GraphPad Instant, version 3.0 (2003) and progesterone receptor (PR) were scored semi-quantitatively. Results: Dexamethasone treatments had no significant (p > 0.05) effect on progesterone and estrogen concentrations in pregnant Sahel goats but up regulated PR from 2+ to 3+ in second trimester. Conclusion: As dexamethasone adverse effect on placenta is an established fact, the lack of effect on progesterone level in this study may be due to the fact that unlike other species whose progesterone production during pregnancy is placenta - dependent, in goats is corpus luteum - dependent. Consequently dexamethasone adverse effect on placenta reported in literatures did not influence progesterone levels during pregnancy in Sahel goat. The up regulation of progesterone receptor (PR) in Sahel goat gravid uterus is a beneficial effects and that dexamethasone can safely be used in corpus luteum - dependent progesterone secreting pregnant animal species like Sahel goat and camel. Therefore source of progesterone secretions during pregnancy should be considered in clinical application of dexamethasone in pregnancy.

Keywords

References

  1. Rothchild I. Roles of progesterone in initiating and maintaining pregnancy. In: Bardin CW, Milgrom E, Mauvais-Jarvis P, editors. Progesterone and Progestin. New York: Raven Press; 1983. p. 219-20.
  2. Beyer C, Gonzalez MG. In: Negro-Vilar A, Perezpalacios G, editors. Hormones, Reproduction, Growth and Development. New York: Raven Press; 1991. p. 58.
  3. Bearden HJ, John WE. Biochemical. haematological and histopathological studies of the extract of Ageratitm conyzoides L. In: Sprague Dawley: Rats, 1997; J. Med. Plant Res 4(2) 11:2264-11:2272.
  4. Luconi M, Bonaccorsi L, Maggi M, Pecchioli P, Kruasz C, Forth G, et al. Identification and characterization of functional nongenomic progesterone receptors on human sperm membrane. J Clin Endo Metab. 1998;86(3):877-85.
  5. Hill RA, Pompolo S, Jones ME, Simpson ER, Boon WC. Estrogen deficiency and apoptosis in dopaminergic neurons in the medial preoptic and arcuate nuclei of male mice. Mol Cell Neuro Sci. 2004;27(2):466-76. https://doi.org/10.1016/j.mcn.2004.04.012
  6. Frandson RD. The Digestive system, In: Anatomy and Physiology of Farm animals. Philadelphia: Lea and Febiger; 1976. p. 242-7.
  7. Dickson WM. Endocrinology, reproduction and lactation. In: Dukes Physiology of Domestic Animals, 11th edn. Melvin J, Swensen D, Williams O, editors., Reece Comstock Publishing Associates. Ithaca: Cornell University Press. 1993;629-64.
  8. Jones SA, Brooks AN, Alfred JR. Steroids modulation of corticotropinreleasing hormone production in human fetal membranes and placenta. J Clin Endocrinol Metab. 1989;68:25-30.
  9. Mesiano S, Jaffe RB. Developmental and functional biology of the primate fetal adrenal cortex. Endocr Rev. 1997;18:378-403.
  10. Nduka N. Clinical Biochemistry for Students of Chemical Pathology. 1st ed. Lagos: Longman; 1997. p. 122-3.
  11. Hunt JS, Miller L, Platt JS. Hormonal regulation of uterine macrophages. Dev Immunol. 1998;6:105-10. https://doi.org/10.1155/1998/87527
  12. Perrot AM, Cohen SK, Milgrom E, Finet M. Progesterone receptor expression in humans. Circulation. 1995;92:2975-83. https://doi.org/10.1161/01.CIR.92.10.2975
  13. Ykijarvinen H, Wahlstrom T, Seppala M. Human endometrium relaxin and progesterone dependent. Acta Obstet Gynecol Scand. 1985;64:663-5. https://doi.org/10.3109/00016348509158210
  14. Milgrom E, Luu MT, Atger M, Baulieu EE. Mechanism regulating the concentration and conformation of progesterone receptors in the uterus. J Biol Chem. 1973;24:66-7.
  15. Vuhai MT, Logear F, Warenbourg M, Milgrom E. Hormonal control of progesterone receptors. Ann N Y Acad Sci. 1977;286:199-209. https://doi.org/10.1111/j.1749-6632.1977.tb29417.x
  16. Schmidt TJ, Meyer AJ. Autoregulation of corticosteroid receptors. Receptor. 1994;4:229-57.
  17. McDonald AR, Maddux BA, Okabayashi Y, Wong KY, Hawley DM, Logsdon CD, et al. Hyperglycemic effects of glucocorticoids. Diabetes. 1987;36:779-81. https://doi.org/10.2337/diab.36.6.779
  18. McDonald IF. Adrenal cortex. In: Veterinary Endocrinology and Reproduction by McDonald I.E. 3rd ed. Philadelphia: Lea and Febiger Co., Inc.; 1990. p. 191.
  19. Aliu YO. Endocrine Pharmacology. In: Veterinary Pharmacology by Aliu. 1st ed. Zaria: Tamaza Publishing Company Ltd; 2007. p. 304-6.
  20. Liggins GC, Howie RN. Controlled trial of antepartum glucocorticoid treatment for prevention of the respiratory distress syndrome (RDS) in premature infants. Pediatrics. 1972;51(6):317-22.
  21. Christer BL. Reports on the consensus development conference on pregnancy. J Reprod Fertic. 1994;29:409-13.
  22. Pierre-Louis T. Anti-inflammatory agents. In: Synthia MK, editor. Merck Veterinary Manual. 10th ed. N.J: Merck and Co., Inc. White House Station; 2010. p. 2313-28.
  23. Sanhu SH. Essentials of Veterinary Pharmacology and Pherapeutics. 2nd ed. New Delhi: Kalyani Publishers Ltd; 2013. p. 776.
  24. Yahi D, Ojo NA, Mshelia GD, Maina VA, Mahre MB. Effects of dexamethasone on leukocytic responses in pregnant Yankasa ewes and Sahel does in Maiduguri, Nigeria. Sokoto J Vet Sci. 2016;14(3):40-6.
  25. Yahi D, Ojo NA, Mshelia GD, Maina VA. Comparative effects of dexamethasone on placental and foetal organ weights and some linear body measurements in Yankasa sheep and Sahel goats. Sokoto J Vet Sci. 2017;15(1):49-56.
  26. Jaudas U, Mobini S. Flow to determine age. In: The Goat Handbook. Flauppauge: Barron's Educational Series Inc; 2006. p. 19-20.
  27. Akusu MO, Egbunike GN. Fertility of the West African Dwarf goat in its native environment following prostaglandin $F_2$- alpha induced estrus. Vet Q J. 1984;6(3):173-6. https://doi.org/10.1080/01652176.1984.9693933
  28. Franco A, Masot AI, Gmez L, Redondo E. Morphometric and Imunohistochemical Study of the Rumen of Red deer during prenatal development. J Anat. 2004;5(1):5-13.
  29. Diest PJ, Weger AR, Lindholm J. Reproducibility of subjective immunoscoring of steroid receptors in breast cancer. Analyt Quant Cytol Histol. 1996;18:351-4.
  30. GraphPad Instat, (2003). Graph Pad Instat Software (2003), version 3.0. GraphPad Software, Inc., San Diego, California, U.S.A., http://www.graphpad.com/, retrieved 25-06-2004.
  31. Ohrlander S, Gennser G, Batra S. Effects of detamethasone administration on estrone, estradiol-$17{\beta}$, and progesterone in maternal plasma and amniotic fluid. Obstet Gynecol. 1977;49:148-53.
  32. Ahmadabad HN, Jafari SK, Firizi MN, Abbaspour AR, Gharib FG, Ghobadi Y, et al. Pregnancy outcome following administration of high doses of dexamethasone in early pregnancy in Mice. Clin Exp Reprod Med. 2016; 43(1):15-25. https://doi.org/10.5653/cerm.2016.43.1.15
  33. Moore NW, Barrett S, Brown JB. Progesterone concentrations in maternal and foetal blood plasma of ewes. J Endocrinol. 1972;5:87-94.
  34. Lye SJ. Initiation of Parturition. Anim Reprod Sci. 1996;42:495-503. https://doi.org/10.1016/0378-4320(96)01529-1
  35. Gordon I. Controlled Reproduction in Sheep and Goats. In: Controlled Reproduction in Farm Animals Series, Vol. 2. 1st ed. United Kingdom: CAB International; 1997. p. 398-415.
  36. McDonald TJ, Franko KL, Brown JM, Jenkins SL, Nathanielsz PW, Nijland MJ. Dexamethasone in the last week of pregnancy causes fetal growth retardation but not adult hypertension in rats. J Soc Gynecolo Invest. 2003;32:469-73.
  37. Ain RL, Canham N, Soares MJ. Dexamethasone-induced intrauterine and placenta growth restriction and impacts on insulin-like growth factor-II and the Akt signaling pathway. J Endocrinol. 2005;185:255-63.
  38. Hewitt DP, Mark PJ, Waddell BJ. Glucocorticoids prevention of normal increase in placental vascular endothelial growth factor expression and placental vascularity during late pregnancy in the rat. Endocrinology. 2006;14(7):56-64.
  39. Ylikorkala O, Dawood MY, Kauppila A. Effects of maternal dexamethasone therapy on the levels of oestrogens, progesterone and chorionic gonadotrophin in amniotic fluid and maternal serum. Br J Obstet Gynaecol. 1978;85:334-7. https://doi.org/10.1111/j.1471-0528.1978.tb14889.x
  40. Schreiber JR, Nakamura K, Erickson GF. Rat ovary and glucocorticoid receptor: identification and characterization. Steroids. 1982;39(5):569-84. https://doi.org/10.1016/0039-128X(82)90057-5
  41. Tetsuka M. Expression of 11-beta-hydroxysteroid dehydrogenase, glucocorticoid receptor and mineralocorticoid receptor genes in rat ovaries. Biol Reprod. 1999;60(2):330. https://doi.org/10.1095/biolreprod60.2.330
  42. Thurston LM. Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles. J Reprod. 2002;124(6):801-12. https://doi.org/10.1530/rep.0.1240801
  43. Kraus MA, Katzenellenbogen NV. Regulation of progesterone receptor gene expression in rat uterus: modulation of estrogen action by progesterone and sex steroid hormone antagonists. Endocrinology. 1993;132:2371-8. https://doi.org/10.1210/endo.132.6.8504742
  44. Leavitt WW, Chen TJ, Allen TC, Johnston JO. Regulation of progesterone receptor formation by estrogen action. Ann N Y Acad Sci. 1977;86:210-25.
  45. Flint APF, Sheldrick EL, Fisher PA. Ligand independent activation of steroid receptors. Domest Anim Endocrin. 2002;23:13-24. https://doi.org/10.1016/S0739-7240(02)00141-8
  46. John S, Gounarides F, Marion KA, Karen K, Gregory A, Oliver T, et al. Effect of Dexamethasone on Glucose Tolerance and Fat Metabolism in a Diet-Induced Obesity Mouse Model. Endocrinology. 2008;149(2):758-66. https://doi.org/10.1210/en.2007-1214

Cited by

  1. Expression of TGF-β1 and miR-99a in serum of patients with early spontaneous abortion and correlation with hormone levels during pregnancy vol.17, pp.6, 2017, https://doi.org/10.3892/etm.2019.7477