Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Investigation of Antioxidant Activity of Cynanchi Wilfordii Radix and Inhibitory Effect of 5α-reductase mRNA in Human Dermal Papilla Cells

백수오(白首烏)의 항산화 효능 및 모유두 세포의 5α-reductase mRNA 발현 억제 효능

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Choi, Young Ho (Department of Biochemistry, Chungnam National University) ;
  • Jeon, Hai Li (Department of Biochemistry, Chungnam National University) ;
  • Lee, Woong Hee (Institute of Biotechnology, Chungnam National University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, College of Social Sciences, Joongbu University)
  • Received : 2017.09.13
  • Accepted : 2017.12.05
  • Published : 2017.12.25
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Abstract

Hair loss affects interpersonal relationships and causes psychological stress. In this study, we investigated the antioxidant activity of Cynanchi Wilfordii Radix (CWR) and its effects on dermal papilla (DP) cells. Antioxidant efficacy was examined by ABTS assay. To confirm the effect on cell activity, MTS assay was performed and cell count was directly measured by hemocytometer. The mRNA expression of genes involved in hair formation and hair loss formation was confirmed by quantitative RT-PCR. CWR has a strong antioxidant activity. Cell viability of DP cells was increased to 118.5% by treatment of 0.5 mg/ml CWR for 24 hours, but the effect on the cell number was insignificant. These results suggest that CWR increases mitochondrial activity without promoting cell proliferation. Treatment of DP cells with 0.5 mg/ml CWR resulted in 48.5% reduction of mRNA expression of type 2 $5{\alpha}$-reductase, a major cause of male hair loss. In addition, mRNA expression of bone morphogenetic pretein (BMP), fibroblast growth factor (FGF)7, and FGF10, which are closely related to hair growth, was also decreased. Reactive oxygen species (ROS) acts as a cause of hair loss. The excellent antioxidant efficacy of CWR is thought to be able to effectively remove ROS. The dihydrotestosterone produced by type 2 $5{\alpha}$-reductase in DP cells is a potent inducer of male pattern hair loss. The inhibitory effect of type 2 $5{\alpha}$-reductase mRNA on DP cells induced by CWR may induce a positive therapeutic effect of male pattern hair loss.

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References

  1. Doh EJ, Kim JH, Choi GY, Lee SH, Song HJ, Ju YS, Lee GS. Microscopic Identification-keys for Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix. Kor. J. Herbol. 2015;30(4):65-9. https://doi.org/10.6116/kjh.2015.30.4.65.
  2. State Administration of Traditional chiense medicine of the People's Republic of China. Zhonghuabencao. Shanghai: Shanghai Scientific and Technical Publishers. 1999:355.
  3. Korea Food and Drug Administration. The Korean Herbal Pharmacopoeia. Seoul: Korea Food and Drug Administration. 2017:275-7.
  4. Kim DH, Kim HM, Ryu JH, Eom JY, Kim SC, Yang JH, Cho MK, Lim JP, Hong SH. Hanbangyakrihak. Seoul:Shinilbukseu. 2007:709-11.
  5. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, LeeYC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY,. Boncho-Hak. Seoul:Young-Lim Press. 2007:635-7.
  6. Chang KC, Lee DU. Vasodilatory Effect of the Alkaloid Component from the Roots of Cynanchum wilfordi Hemsley. Korean Journal of Life Science. 2000;10(6):584-90.
  7. Shin MK. A Comparative Study on the Effects of Polygoni Radix and Cynanchi Radix on Rat Livers Intoxicated with Carbon Tetrachloride. Kor J Pharmacogn. 1985; 16(2):81-92.
  8. Choi JH, Lee Hs, Kim YE, Kim BM, Kim IH, Lee CH. Effect of Cynanchi wilfordii Radix Extracts on Lipid Compositions and Blood Pressure in Spontaneously Hypertensive Rats. J Korean Soc Food Sci Nut. 2012;41(3);345-50. https://doi.org/10.3746/jkfn.2012.41.3.345
  9. Ahn YH, Oh JM, Lee MS, Jung JH, Chae SU, Moon SY, Jeon BH, Choi MK. Effect of Water Extract of Cynanchi Wilfordii Radix in RANKL-induced Osteoclast Differentiation. Korean J Oriental Physiology & Pathology 2012;26(2):160-5.
  10. Kim HS. Effects of Cynanchum wilfordii Extract on Serum Lipid Components and Enzyme Activities in Hyperlipidemic and Streptozotocin-Induced Diabetic Rats. Korean J Human Ecology. 2004;7(2):1-11.
  11. Lindner G, Botchkarev VA, Botchkareva NV, Ling G, van der Veen C, Paus R. Analysis of apoptosis during hair follicle regression(catagen). Am J Pathol. 1997;151(6):1601-7.
  12. Takahashi T, Kamiya T, Yokoo Y. Proanthocyanidins from grape seeds promote proliferation of mouse hair follicle cells in vitro and convert hair cycle in vivo. Acta Derm Venereol. 1998;78(6):428-32. https://doi.org/10.1080/000155598442719
  13. Jang HY, Choi KH, Kim SH, Kwon KR, Kim BW. Bibliog raphic Studies of Depilation. J Pharmacoacupuncture. 2002;5(2):92-108. https://doi.org/10.3831/KPI.2002.5.2.092
  14. Zhongyiyanjiuyuan. Zhongyizhengzhuangjianbiezhenduanx ue. Beijing:Renminweisheng chubanshe. 1987:495-6.
  15. Yang SS. Zhongyilinchuangdaquan. Beijing: Beijingkexue jishuchubanshe. 1965:911-2.
  16. Chen GY, Yang SS. Shiyongzhongxiyijiehezhenduanzhilia oxue. Beijing:Zhongguoyiyaokejichubanshe. 1991:1508,1510.
  17. Gu BK. Zhongyiwaikexue. Beijing:Renminweisheng chubanshe. 1987:309-12.
  18. Lee BG. Jeungsanggambyeolchilyo. seoul:Seongbosa. 1991: 773-4.
  19. Park JS, LeeJS. The Promoting Effect of Pleuropterus cilinervis Extracts Fermented with Lactobacillus rhamnosus on Hair Growth. Korean J Microbiol Biotechnol. 2011;39(4):345-9.
  20. Kim CD, Lee MH, Roh SS. Identification of androgen-regulated genes in SV40-transformed human hair dermal papilla cells. J. Dermatol. Sci. 2003;32(2):143-9. https://doi.org/10.1016/S0923-1811(03)00093-8
  21. Ralph M Trueb. Oxidative Stress in Ageing of Hair. Int J Trichology. 2009;1(1):6-14. https://doi.org/10.4103/0974-7753.51923
  22. Randall VA. Androgen and hair growth. Dermatol Ther. 2008;21(5):314-28. https://doi.org/10.1111/j.1529-8019.2008.00214.x
  23. Solanas G, Benitah SA. Regenerating the skin: a task for the heterogeneous stem cell pool and surrounding niche. Nat Rev Mol Cell Biol. 2013;14(11):737-48. https://doi.org/10.1038/nrm3675
  24. Enshell-Seijffers D, Lindon C, Kashiwagi M, Morgan BA. beta-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair. Dev Cell. 2010;18(4):633-42. https://doi.org/10.1016/j.devcel.2010.01.016
  25. Lei MX, Chuong CM, Widelitz RB. Tuning Wnt Signals for More or Fewer Hairs. J Invest Dermatol. 2013;133(1):7-9. https://doi.org/10.1038/jid.2012.446
  26. Heo J. Donguibogam. Namsandang;Seoul. 1989:735.
  27. Lee CB. Daehansigmuldogam. Hyangmunsa. 1982:630.
  28. Korea Food & Drug Administration. Search for a publication data base. from, https://www.mfds.go.kr/herbmed/index.do?nMenuCode=8&page=2&page=2&mode=view&boardSeq=71515.
  29. Hamilton JB. Male hormone stimulation is a prerequisite and an incitant in common baldness. Am J Anat. 1942;71:451. https://doi.org/10.1002/aja.1000710306
  30. Naito A, Midorikawa T, Yoshino T, Ohdera M. Lipid peroxides induce early onset of catagen phase in murine hair cycles. Int J Mol Med. 2008;22(6):725-9.
  31. Liu N, Wang LH, Guo LL, Wang GQ, Zhou XP, Jiang Y, Shang J, Murao K, Chen JW, Fu WQ, Zhang GX. Chronic Restraint Stress Inhibits Hair Growth via Substance P Mediated by Reactive Oxygen Species in Mice. PLoS One. 2013;8(4):e61574. https://doi.org/10.1371/journal.pone.0061574
  32. Rahmani W, Abbasi S, Hagner A, Raharjo E, Kumar R, Hotta A, Magness S, Metzger D, Biernaskie J. Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type. Dev Cell. 2014;31(5):543-58. https://doi.org/10.1016/j.devcel.2014.10.022
  33. Dunigan DD, Waters SB, Owen TC. Aqueous soluble tetrazolium/formazan MTS as an indicator of NADHand NADPH-dependent dehydrogenase activity. Biotechniques. 1995;19(4):640-9.
  34. Azzouni F, Godoy A, Li Y, Mohler J. The 5 alpha-reductase isozyme family: a review of basic biology and their role in human diseases. Adv Urol. 2012;2012:530121.
  35. Kaufman KD. Olsen EA, Whiting D, Savin R, DeVillez R, Bergfeld W, Price VH, Neste DV, Roberts JL, Hordinsky M, Shapiro J, Binkowitz B, Gormley GJ, the Finasteride Male Pattern Hair Loss Study Group. Finasteride in the treatment of men with androgenetic alopecia. Journal of the American Academy of Dermatology. 1998;39(4):578-89. https://doi.org/10.1016/S0190-9622(98)70007-6
  36. Kang JI, Kim SC, Kim MK, Boo HJ, Kim EJ, Im GJ, Kim YH, Hyun JW, Kang JH, Koh YS, Park DB, Yoo ES, Kang HK. beta2 in catagen induction during the human hair cycle. Molecular and cellular pharmacology. 2015;757:74-83.
  37. Ferrando J, Grimalt R, Alsina M, Bulla F, Manasievska E. Unilateral gynecomastia induced by treatment with 1 mg of oral finasteride. Arch Dermatol. 2002;138(4):543-4. https://doi.org/10.1001/archderm.138.4.533