생명과학회지 (Journal of Life Science)

  • 제27권4호
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  • Pages.456-463
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

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한약재 추출물의 에스트로겐 유사활성 및 조골세포 증식과 분화에 미치는 영향

The Effects of Medicinal Herbs Extracts on Estrogen-like Activities and Osteoblast Proliferation and Differentiation

  • 김미향 (신라대학교 의생명과학대학 식품영양학과) ;
  • 김보경 (신라대학교 의생명과학대학 식품영양학과) ;
  • 김재덕 (신라대학교 일반대학원 그린화학융합공학과) ;
  • 강아람 (신라대학교 의생명과학대학 식품영양학과) ;
  • 이창은 (신라대학교 일반대학원 그린화학융합공학과) ;
  • 서정민 (신라대학교 의생명과학대학 식품영양학과) ;
  • 이동근 (신라대학교 일반대학원 바이오과학과) ;
  • 조정권 (신라대학교 일반대학원 바이오과학과) ;
  • 김육용 ((주)이삭에프앤비) ;
  • 유기환 ((주)이삭에프앤비) ;
  • 이상현 (신라대학교 일반대학원 그린화학융합공학과)
  • Kim, Mihyang (Department of Food and Nutrition, Silla University) ;
  • Kim, Bokyung (Department of Food and Nutrition, Silla University) ;
  • Kim, Jae-Deog (Department of Green-Chemistry Convergence Engineering, Silla University) ;
  • Kang, A-Ram (Department of Food and Nutrition, Silla University) ;
  • Lee, Chang-Eun (Department of Green-Chemistry Convergence Engineering, Silla University) ;
  • Seo, Jungmin (Department of Food and Nutrition, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience, Graduate School, Silla University) ;
  • Jo, Jung-Kwon (Department of Bioscience, Graduate School, Silla University) ;
  • Kim, Yuck Yong (ISAAC F&B Co., Marine Bio-industry Department Center) ;
  • Yu, Ki Hwan (ISAAC F&B Co., Marine Bio-industry Department Center) ;
  • Lee, Sang-Hyeon (Department of Green-Chemistry Convergence Engineering, Silla University)
  • 투고 : 2017.02.08
  • 심사 : 2017.02.24
  • 발행 : 2017.04.30
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초록

본 연구는 3종의 한약재(감초(Glycyrrhizae radix), 황기(Astragali radix) 및 산약(Dioscorea rhizome)) 추출물의 에스트로겐 유사활성 및 조골세포 증식과 분화에 미치는 영향을 검토하고자 실험을 진행하였다. 에스트로겐 유사활성 측정을 위하여 인체 유방암 세포주인 MCF7 세포를 luciferase 유전자를 리포터로 사용하는 에스트로겐 반응성 리포터 시스템을 가지는 세포로 형질전환하여 사용하였다. 상기 세포를 이용하여 추출물의 에스트로겐 유사활성을 측정한 결과, 한약재 추출물이 음성대조군과 비교하여 1.11배~5.73배 높은 에스트로겐 유사활성을 나타내었다. 그 중 감초 추출물이 가장 높은 에스트로겐 유사활성을 보였다. 감초 추출물의 에스트로겐 유사활성은 추출물의 농도가 $50{\mu}g/ml$ 및 $500{\mu}g/ml$ 일 때 각각 $10^{-8}M$ 및 $10^{-7}M$ $17{\beta}-estradiol$과 거의 유사한 활성을 나타내었다. 조골세포주인 MC3T3-E1 세포를 이용한 실험에서는 감초 추출물의 농도가 $1{\sim}1,000{\mu}g/ml$ 범위일 때 독성을 나타내지 않았다. 황기 추출물은 조골세포에 있어서 유의적인 세포 증식률을 보이지 않았다. 그러나, 감초 및 산약 추출물은 각각 148% 및 133%의 최대 증식률을 보였다. 또한, 감초 추출물은 대조군과 비교하여 alkaline phosphatase(ALP) 활성이 증가하였으며, 추출물의 농도가 $100{\mu}g/ml$ 일 때 최대 122%의 ALP 활성을 나타내었다. 또한, Alizarin red S 염색법을 이용한 석회화 형성능 측정에서도 대조군과 비교하여 석회화가 증가하였다. 이러한 결과로 미루어 보아 감초 추출물이 골 형성 및 골다공증 예방 효과가 우수한 식품 소재인 것으로 사료된다.

The purpose of this study was to investigate the effect of 3 types of medicinal herbs (Glycyrrhizae radix, Astragali radix and Dioscorea rhizoma) extracts on estrogen-like activities, proliferation and differentiation in osteoblast. Human breast cancer cell line MCF7 was transfected using an estrogen responsive luciferase reporter plasmid for measure the estrogen-like activity. Estrogen-like activities of extracts were in the range of 1.11~5.73 fold to that of negative control. The extract of G. radix showed the strongest estrogen-like activities. The estrogen-like activities of 50 and $500{\mu}g/ml$ extracts of G. radix were similar to that of $10^{-8}$ and $10^{-7}$ M standard solution ($17{\beta}-estradiol$), respectively. G. radix extract showed no cytotoxicity against osteoblast MC3T3-E1 cells at $1{\sim}1,000{\mu}g/ml$. The extract of A. radix showed no significant proliferation of osteoblast. However, the extract of G. radix and D. rhizome showed maximum 148% and 133% proliferation effects. The extract of G. radix also increased alkaline phosphatase activity and the maximum was 122% at $100{\mu}g/ml$ compared to that of control. The nodule formation by the method of the Alizarin red S staining increased compared to control. These results suggest that G. radix is able to perform the bone formation and prevent osteoporosis.

키워드

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