Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Comparison of Anti-asthmatic Activity by Native Codonopsis lanceolata Extract

자생 돌더덕 추출물에 의한 천식억제 활성의 분석

  • Lee, Seung-Ha (Departments of Food Industrial Engineering, Graduate School, Kyungpook National University) ;
  • Choi, Hee-Jeong (Departments of Food Science and Biotechnology, Graduate School, Kyungpook National University) ;
  • Heo, Jin-Chul (Departments of Biomedical Engineering, School of Medicine, Keimyung University) ;
  • Lee, Jong-Ha (Departments of Biomedical Engineering, School of Medicine, Keimyung University) ;
  • Kwon, Taeg Kyu (Departments of Immunology, School of Medicine, Keimyung University) ;
  • Ha, Sang-Chul (Department of Hotel Culinary Arts, Daegu Mirae College) ;
  • Lee, Sang-Han (Departments of Food Industrial Engineering, Graduate School, Kyungpook National University)
  • 이승하 (경북대학교 대학원 식품산업공학전공) ;
  • 최희정 (경북대학교 대학원 식품생물공학전공) ;
  • 허진철 (계명대학교 의과대학 의용공학) ;
  • 이종하 (계명대학교 의과대학 의용공학) ;
  • 권택규 (계명대학교 의과대학 면역학교실) ;
  • 하상철 (대구미래대학교 호텔외식조리과) ;
  • 이상한 (경북대학교 대학원 식품산업공학전공)
  • Received : 2017.02.02
  • Accepted : 2017.04.04
  • Published : 2017.04.30
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Abstract

Codonopsis lanceolata (Campanulaceae) has been widely used in traditional medicine and is considered to have medicinal properties to treat diseases and symptoms such as bronchitis, coughs, spasm, edema, hepatitis, colitis, and lung injury. In order to investigate whether native Codonopsis lanceolata extract alleviates ashmatic symptoms in vivo, we first carried out various antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC) assays. The antioxidant activities were increased by adding Codonopsis lanceolata extract in a concentration-dependent manner which compared to ascorbic acid as a positive control. Histological studies using an ovalbumin-induced animal model exhibited potent anti-inflammatory potential by decreasing immuno-responsive cells in the lung by the extract by confirming H&E and PAS staining. It is revelaed that further immunihistochemical analysis showed anti-ashmatic capabilities by assessing histamine, IL-31, and MMP-9 expressions. The level of IL-13 expression in Codonopsis lanceolata extract-treated group was decreased upto 73.7% compared to control, whereas that of total cells and eosinophil counting in Codonopsis lanceolata extract-treated group was diminished to 73.5% and 80.9%, respectively. These results collectively indicate that the C. lanceolata extract ameliorates asthmatic symptoms effectively in an ovalbumin-challenged mice model, in that the extract can be used for the development of an anti-asthmatic food ingredient.

돌더덕은 전통의학의 소재로서 기관지염, 기침, 경련, 부종, 간염, 대장염, 폐 손상의 치료로 사용되어 왔다. 돌더덕 추출물의 천식증상 완화를 연구하기 위하여, 먼저 항산화 활성을 비교한 결과, DPPH, FRAP, Cupper 소거활성 실험으로부터 농도 의존적으로 우수한 활성을 확인할 수 있었다. 오브알부민 유도의 마우스 천식 동물모델에 적용한 결과, histamine, IL-31, MMP-9의 발현이 현저하게 감소함을 알 수 있었다. 또한 IL-13, 면역세포수, 호산구의 발현이 73.7%, 73.5%, 80.9% 정도 억제되었다. 이의 결과로, 돌더덕 추출물은 오브알부민 유도의 마우스 천식 동물모델에서 탁월한 천식 효과를 나타내므로 항천식 식품소재나 바이오소재로의 개발이 기대된다.

Keywords

References

  1. Ajaikumar, K, B., Asheef, M., Babu, B. H. and Padikkala, J. 2005. The inhibition of gastric mucosal injury by Punicagranatum L. (pomegranate) methanolic extract. J. Ethnopharmacol. 4, 171-176.
  2. Byeon, S. E., Choi, W. S., Hong, E. K., Lee, J., Rhee, M. H., Park, H. J. and Cho, J. Y. 2009. Inhibitory effect of saponin fraction from C. lanceolata on immune cell-mediated inflammatory responses. Arch. Pharm. Res. 32, 813-822. https://doi.org/10.1007/s12272-009-1601-7
  3. Cha, A., Choi, Y., Jin, Y., Sung, M. K., Koo, Y. C., Lee, K. W. and Park, T. 2012. Antilipogenic and anti-inflammatory activities of C. lanceolata in mice hepatic tissues after chronic ethanol feeding. J. Biomed. Biotechnol. 2012, 141395.
  4. Chen, J., Small-Howard, A., Yin, A. and Berry, M. J. 2005. The responses of Ht22 cells to oxidative stress induced by buthionine sulfoximine. BMC Neurosci. 12, 6-10.
  5. Heo, J. C., Park, J. Y., Hwang, J. S., Park, H. C., Kang, S. W., Hwang, S. J., Yun, C. Y., Kwon, T. K. and Lee, S. H. 2006. Comparison of in vitro antioxidant activity and cyclooxygenase- 2 promoter inhibitory activity in H. axyridis Pallas and C. septempunctata L. Kor. J. Food Preserv. 13, 513-518.
  6. Heo, J. C., Park, J. Y., Kwon, T. K., Chung, S. K., Kim, S. U. and Lee, S. H. 2005. Development of high throughput screening techniques using food-borne library against antiasthma agents. Kor. J. Food Preserv. 12, 267-274.
  7. Heo, J. C., Woo, S. U., Kweon, M. A., Park, J. Y., Lee, H. K., Son, M., Rho, J. R. and Lee, S. H. 2008. Aqueous extract of the H. annuus seed alleviates asthmatic symptoms in vivo. Int. J. Mol. Med. 21, 57-61.
  8. Joh, E. H. and Kim, D. H. 2010. Lancemaside A inhibits lipopolysaccharide- induced inflammation by targeting LPS/ TLR4 complex. J. Cell Biochem. 111, 865-871. https://doi.org/10.1002/jcb.22773
  9. Kim, M. H., Lee, J., Yoo, D. S., Lee, Y. G., Byeon, S. E., Hong, E. K. and Cho, J. Y. 2009. Protective effect of stress-induced liver damage by saponin fraction from C. lanceolata. Arch. Pharm. Res. 32, 1441-1446. https://doi.org/10.1007/s12272-009-2014-3
  10. Kim, N. Y., Chae, H. S., Lee, I. S., Kim, D. S., Seo, K. T. and Park, S. J. 2010. Analysis of chemical composition and antioxidant activity of C. lanceolata skin. J. Kor. Soc. Food Sci. Nutr. 39, 1627-1633. https://doi.org/10.3746/jkfn.2010.39.11.1627
  11. Lee, K. T., Choi, J., Jung, W. T., Nam, J. H., Jung, H. J. and Park, H. J. 2002. Structure of a new echinocystic acid bisdes moside isolated from C. lanceolata roots and the cytotoxic activity of prosapogenins. J. Agric. Food Chem. 50, 4190-4193. https://doi.org/10.1021/jf011647l
  12. Lee, Y. G., Kim, J. Y., Lee, J. Y., Byeon, S. E., Hong, E. K., Lee, J., Rhee, M. H., Park, H. J. and Cho. J, Y, 2007. Regulatory effects of C. lanceolata on macrophage- mediated immune responses. J. Ethnopharmacol. 112, 180-188. https://doi.org/10.1016/j.jep.2007.02.026
  13. Li, J. P., Liang, Z. M. and Yuan, Z. 2007. Triterpenoid saponins and anti-inflammatory activity of C. lanceolata. Pharmazie 62, 463-466.
  14. Liu, M. L. and Hong, S. T. 2005. Early phase of amyloid beta42-induced cytotoxicity in neuronal cells is associated with vacuole formation and enhancement of exocytosis. Exp. Mol. Med. 37, 559-566. https://doi.org/10.1038/emm.2005.69
  15. MacNee, W. 2001. Oxidative stress and lung inflammation in airways disease. Eur. J. Pharmacol. 429, 195-207. https://doi.org/10.1016/S0014-2999(01)01320-6
  16. Nadeem, A., Chhabra, S. K., Masood, A. and Raj, H. G. 2003. Increased oxidative stress and altered levels of antioxidants in asthma. J. Allergy Clin. Immunol. 111, 72-78. https://doi.org/10.1067/mai.2003.17
  17. Park S. J., Song, S. W., Seong, D. H., Park, D. S., Kim, S. S., Jinyu, G., Ahn, J. H., Yoon, W. B. and Lee, H. Y. 2009. Biological activities in the extract of fermented Codonopsis lanceolata. J. Kor. Soc. Food Sci. Nutr. 38, 983-988. https://doi.org/10.3746/jkfn.2009.38.8.983
  18. Park, S. J., Seong, D. H., Park, D. S., Kim, S. S., Jinyu, G., Ahn, J. H., Yoon, W. B. and Lee, H. Y. 2009. Chemical compositions of fermented Codonopsis lanceolata. J. Kor. Soc. Food Sci. Nutr. 38, 396-400. https://doi.org/10.3746/jkfn.2009.38.3.396
  19. Shin, S. C., Lee, S. R., Yoon, E. S. and Lee, Y. S. 1991. Proximate and mineral of dried wild and cultivated C. lanceolata Benth et Hook Fil. of different cultivated groups. Kor. J. Plant Res. 4, 39-45.
  20. Trottein, F, Mallevaey, T, Faveeuw, C, Capron, M. and Leite-de-Moraes, M. 2006. Role of the natural killer T lymphocytes in Th2 responses during allergic asthma and helminth parasitic diseases. Chem. Immunol. Allergy 90, 113-127.

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