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PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates

  • Kang, Jung-Mi (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Lee, Jinyoung (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kim, Tae Im (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Koh, Eun-Ha (Department of Laboratory Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kim, Tong-Soo (Department of Tropical Medicine and Inha Research Institute for Medical Sciences, Inha University School of Medicine) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Na, Byoung-Kuk (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University School of Medicine)
  • Received : 2016.12.19
  • Accepted : 2017.02.19
  • Published : 2017.04.30

Abstract

Vivax malaria reemerged in Korea in 1993 and the outbreak has been continued with fluctuating numbers of annual indigenous cases. Understanding the nature of the genetic population of Plasmodium vivax circulating in Korea is beneficial for the knowledge of the nationwide parasite heterogeneity and in the implementation of malaria control programs in the country. Previously, we analyzed polymorphic nature of merozoite surface protein-1 (MSP-1) and MSP-$3{\alpha}$ in Korean P. vivax population and identified the Korean P. vivax population has been diversifying rapidly, with the appearance of parasites with new genetic subtypes, despite the recent reduction of the disease incidence. In the present study, we developed simple PCR-RFLP methods for rapid subtyping of MSP-1 and MSP-$3{\alpha}$ of Korean P. vivax isolates. These PCR-RFLP methods were able to easily distinguish each subtype of Korean P. vivax MSP-1 and MSP-$3{\alpha}$ with high accuracy. The PCR-RFLP subtyping methods developed here would be easily applied to massive epidemiological studies for molecular surveillance to understand genetic population of P. vivax and to supervise the genetic variation of the parasite circulating in Korea.

Keywords

References

  1. Mendis K, Sina BJ, Marchesini P, Carter R. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 2001; 64: 97-106. https://doi.org/10.4269/ajtmh.2001.64.97
  2. Guerra CA, Hay SI, Lucioparedes LS, Gikandi PW, Tatem AJ, Noor AM, Snow RW. Assembling a global database of malaria parasite prevalence for the Malaria Atlas Project. Malar J 2007; 6: 17. https://doi.org/10.1186/1475-2875-6-17
  3. Soh CT, Lee KT, Im KI, Min DY, Ahn MH, Kim JJ, Yong TS. Current status of malaria in Korea. Yonsei Rep Trop Med 1985; 16: 11-18.
  4. Kim TS, Kim JS, Na BK, Lee WJ, Kim HC, Youn SK, Gwack J, Kim HS, Cho P, Ahn SK, Cha SH, Park YK, Lee SK, Kang YJ, Sohn Y, Hong Y, Lee HW. Decreasing incidence of Plasmodium vivax in the Republic of Korea during 2010-2012. Malar J 2013; 12: 309. https://doi.org/10.1186/1475-2875-12-309
  5. Lim CS, Kim SH, Kown SI, Song JW, Song KJ, Lee KN. Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates. Am J Trop Med Hyg 2000; 62: 261-265. https://doi.org/10.4269/ajtmh.2000.62.261
  6. Choi YK, Choi KM, Park MH, Lee EG, Kim YJ, Lee BC, Cho SH, Rhie HG, Lee HS, Yu JR, Lee JS, Kim TS, Kim JY. Rapid dissemination of newly introduced Plasmodium vivax genotypes in South Korea. Am J Trop Med Hyg 2010; 82: 426-432. https://doi.org/10.4269/ajtmh.2010.09-0245
  7. Han ET, Wang Y, Lim CS, Cho JH, Chai JY. Genetic diversity of the malaria vaccine candidate merozoite surface protein 1 gene of Plasmodium vivax field isolates in Republic of Korea. Parasitol Res 2011; 109: 1571-1576. https://doi.org/10.1007/s00436-011-2413-5
  8. Honma H, Kim JY, Palacpac NM, Mita T, Lee W, Horii T, Tanabe K. Recent increase of genetic diversity in Plasmodium vivax population in the Republic of Korea. Malar J 2011; 10: 257. https://doi.org/10.1186/1475-2875-10-257
  9. Kang JM, Ju HL, Kang YM, Lee DH, Moon SU, Sohn WM, Park JW, Kim TS, Na BK. Genetic polymorphism and natural selection in the C terminal 42kDa region of merozoite surface protein-1 among Plasmodium vivax Korean isolates. Malar J 2012; 11: 206. https://doi.org/10.1186/1475-2875-11-206
  10. Ju HL, Kang JM, Moon SU, Bahk YY, Cho PY, Sohn WM, Park YK, Park JW, Kim TS, Na BK. Genetic diversity and natural selection of Duffy binding protein of Plasmodium vivax Korean isolates. Acta Trop 2013; 125: 67-74. https://doi.org/10.1016/j.actatropica.2012.09.016
  11. Kang JM, Ju HL, Cho PY, Moon SU, Ahn SK, Sohn WM, Lee HW, Kim TS, Na BK. Polymorphic patterns of the merozoite surface protein-$3{\beta}$ in Korean isolates of Plasmodium vivax. Malar J 2014; 13: 104. https://doi.org/10.1186/1475-2875-13-104
  12. Goo YK, Moon JH, Ji SY, Chung DI, Hong Y, Cho SH, Lee WJ, Kim JY. The unique distribution of the Plasmodium vivax merozoite surface protein 1 in parasite isolates with short and long latent periods from the Republic of Korea. Malar J 2015; 14: 299. https://doi.org/10.1186/s12936-015-0803-3
  13. Kang JM, Lee J, Cho PY, Moon SU, Ju HL, Ahn SK, Sohn WM, Lee HW, Kim TS, Na BK. Population genetic structure and natural selection of apical membrane antigen-1 in Plasmodium vivax Korean isolates. Malar J 2015; 14: 455. https://doi.org/10.1186/s12936-015-0942-6
  14. Kang JM, Lee J, Chi PY, Kim TI, Sohn WM, Park JW, Kim TS, Na BK. Dynamic changes of Plasmodium vivax population structure in South Korea. Infect Genet Evol 2016; 45: 90-94. https://doi.org/10.1016/j.meegid.2016.08.023
  15. Kim JY, Goo YK, Zo YG, Ji SY, Trimarsanto H, To S, Clark TG, Price RN, Auburn S. Further evidence of increasing diversity of Plasmodium vivax in the Republic of Korea in recent years. PLoS One 2016; 11: e0151514. https://doi.org/10.1371/journal.pone.0151514
  16. Koepfli C, Mueller I, Marfurt J, Goroti M, Sie A, Oa O, Genton B, Beck HP, Felger I. Evaluation of Plasmodium vivax genotyping markers for molecular monitoring in clinical trials. J Infect Dis 2009; 199: 1074-1080. https://doi.org/10.1086/597303
  17. Bruce MC, Galinski MR, Barnwell JW, Snounou G, Day KP. Polymorphism at the merozoite surface protein-$3{\alpha}$ locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 1999; 61: 518-525. https://doi.org/10.4269/ajtmh.1999.61.518
  18. Leclerc MC, Gauthier C, Villegas L, Urdaneta L. Genetic diversity of merozoite surface protein-1 gene of Plasmodium vivax isolates in mining villages of Venezuela (Bolivar State). Acta Trop 2005; 95: 26-32. https://doi.org/10.1016/j.actatropica.2005.03.007
  19. Moon SU, Lee HW, Kim JY, Na BK, Cho SH, Lin K, Sohn WM, Kim TS. High frequency of genetic diversity of Plasmodium vivax field isolates in Myanmar. Acta Trop 2009; 109: 30-36. https://doi.org/10.1016/j.actatropica.2008.09.006
  20. Udagama PV, Gamage-Mendis AC, David PH, Peiris JS, Perera KL, Mendis KN, Carter R. Genetic complexity of Plasmodium vivax parasites in individual human infections analyzed with monoclonal antibodies against variant epitopes on a single parasite protein. Am J Trop Med Hyg 1990; 42: 104-110. https://doi.org/10.4269/ajtmh.1990.42.104
  21. Arevalo-Herrera M, Chitnis C, Herrera S. Current status of Plasmodium vivax vaccine. Hum Vaccin 2010; 6: 124-132. https://doi.org/10.4161/hv.6.1.9931
  22. Mourao LC, Morais CG, Bueno LL, Jimenez MC, Soares IS, Fontes CJ, Guimaraes Lacerda MV, Xavier MS, Barnwell JW, Galinski MR, Braga EM. Naturally acquired antibodies to Plasmodium vivax blood-stage vaccine candidates (PvMSP-119 and PvMSP-$3{\alpha}_{359-798}$ and their relationship with hematological features in malaria patients from the Brazilian Amazon. Microbes Infect 2012; 14: 730-739. https://doi.org/10.1016/j.micinf.2012.02.011
  23. Zakeri S, Barjesteh H, Djadid ND. Merozoite surface protein-$3{\alpha}$ is a reliable marker for population genetic analysis of Plasmodium vivax. Malar J 2006; 5: 53. https://doi.org/10.1186/1475-2875-5-53
  24. Zakeri S, Raeisi A, Afsharpad M, Kakar Q, Ghasemi F, Atta H, Zamani G, Memon MS, Salehi M, Djadid ND. Molecular characterization of Plasmodium vivax clinical isolates in Pakistan and Iran using pvmsp-1, pvmsp-$3{\alpha}$ and pvcsp genes as molecular markers. Parasitol Int 2010; 59: 15-21. https://doi.org/10.1016/j.parint.2009.06.006
  25. Cui L, Mascorro CN, Fan Q, Rzomp KA, Khuntirat B, Zhou G, Chen H, Yan G, Sattabongkot J. Genetic diversity and multiple infections of Plasmodium vivax malaria in Western Thailand. Am J Trop Med Hyg 2003; 68: 613-619. https://doi.org/10.4269/ajtmh.2003.68.613
  26. Cristiano FA, Perez MA, Nicholls RS, Guerra AP. Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia. Mem Inst Oswaldo Cruz 2008; 103: 493-496. https://doi.org/10.1590/S0074-02762008000500015
  27. Veron V, Legrand E, Yrinesi J, Volney B, Simon S, Carme B. Genetic diversity of msp$3{\alpha}$ and msp1_b5 markers of Plasmodium vivax in French Guiana. Malar J 2009; 8: 40. https://doi.org/10.1186/1475-2875-8-40
  28. Prajapati SK, Joshi H, Valecha N. Plasmodium vivax merozoite surface protein-$3{\alpha}$: a high-resolution marker for genetic diversity studies. J Vector Borne Dis 2010; 47: 85-90.
  29. Khan SN, Khan A, Khan S, Ayaz S, Attaullah S, Khan J, Khan MA, Ali I, Shah AH. PCR/RFLP-based analysis of genetically distinct Plasmodium vivax population of Pvmsp-$3{\alpha}$ and Pvmsp-$3{\beta}$ genes in Pakistan. Malar J 2014; 13: 355. https://doi.org/10.1186/1475-2875-13-355
  30. Zakeri S, Djadid ND, Zeinali S. Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in Southeastern Iran. Acta Trop 2003; 88: 91-97. https://doi.org/10.1016/S0001-706X(03)00192-X
  31. Verma A, Joshi H, Singh V, Anvikar A, Valecha N. Plasmodium vivax msp-$3{\alpha}$ polymorphisms: analysis in the Indian subcontinent. Malar J 2016; 15: 492. https://doi.org/10.1186/s12936-016-1524-y