Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Capsanthin Inhibits both Adipogenesis in 3T3-L1 Preadipocytes and Weight Gain in High-Fat Diet-Induced Obese Mice

  • Jo, Sung Jun (Life Science Research Institute, E.S. Biotech. Co. Ltd.) ;
  • Kim, Jeung Won (Life Science Research Institute, E.S. Biotech. Co. Ltd.) ;
  • Choi, Hye Ok (Life Science Research Institute, E.S. Biotech. Co. Ltd.) ;
  • Kim, Jung Hwan (Life Science Research Institute, E.S. Biotech. Co. Ltd.) ;
  • Kim, Hyung Joong (Life Science Research Institute, E.S. Biotech. Co. Ltd.) ;
  • Woo, Sun Hee (Department of Agronomia, Chungbuk National University) ;
  • Han, Byung Hoon (Life Science Research Institute, E.S. Biotech. Co. Ltd.)
  • Received : 2017.03.06
  • Accepted : 2017.03.18
  • Published : 2017.05.01
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Abstract

Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis ($IC_{50}$; $2.5{\mu}M$) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes ($ED_{50}$; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-${\beta}_2$-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss. The capsanthin-induced activation of kinetic behavior in mice was associated with the excessive production of ATP initiated by both the enhanced lipolytic activity together with accelerated oxidation of fatty acids due to the adrenoceptor ${\beta}_2$-agonistic activity of capsanthin. Capsanthin also dose-dependently increased adiponectin and p-AMPK activity in high fat diet animals, suggesting that capsanthin has both anti-obesity and insulin sensitizing activities.

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References

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