Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antimicrobial Activity of Maesil (Prunus mume) Extract against Vibrio vulnificus

비브리오 패혈증균에 대한 매실 추출물의 항균활성

  • Ha, ea-Man (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Jeon, Doo-Young (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Im, Hyun-Chul (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Yoon, Yeon-Hee (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Shin, Mi-Yeong (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Yoon, Ki-Bok (Division of Microbiology, Jeollanam-do Institute of Health and Environment) ;
  • Kim, Jung-Beom (Department of Food Science and Technology, SunChon National University)
  • 하태만 (전라남도보건환경연구원 미생물과) ;
  • 전두영 (전라남도보건환경연구원 미생물과) ;
  • 임현철 (전라남도보건환경연구원 미생물과) ;
  • 윤연희 (전라남도보건환경연구원 미생물과) ;
  • 신미영 (전라남도보건환경연구원 미생물과) ;
  • 윤기복 (전라남도보건환경연구원 미생물과) ;
  • 김중범 (순천대학교 식품공학과)
  • Received : 2017.02.22
  • Accepted : 2017.03.21
  • Published : 2017.04.30
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Abstract

The purpose of this study was to estimate the antimicrobial activity of Maesil (Japanese apricot, Prunus mume) extract against Vibrio vulnificus. The strains tested in the study were 28 V. vulnificus isolates originated from fish, seawater, mud flat and seawater in fish restaurant. The vvhA gene was detected using real-time PCR and biochemical identification expressed above good identification in 28 isolates of V. vulnificus. All of V. vulnificus used in this study was susceptible to tetracycline and chloramphenicol antibiotics. These two antibiotics were considered to be useful for the treatment of patients. Maesil extracts 2.5% and 5% showed antimicrobial activity against V. cholerae NCCP 13589 and V. parahemolyticus NCCP 11143. V. vulnificus isolate and V. vulnificus NCCP 11135 showed growth inhibition at 1.25%, 2.5% and 5% of Maesil extract, respectively. Compared with V. cholerae and V. parahemolyticus, the antibacterial activity of Maesil extract against V. vulnificus was high. The minimum bactericidal concentration of Maesil extract for V. vulnificus was 1.6%. These results revealed that Maesil extract was found to be very useful for inhibiting the growth of V. vulnificus and can be expected to prevent food poisoning caused by V. vulnificus.

V. vulnificus에 대한 매실 추출액의 항균활성을 측정함으로서 다소비 식품인 수산물의 미생물학적 안전성 확보에 기여하고자 하였다. 실험에서 사용한 균주는 수산물, 수족관수, 해수, 갯벌에서 분리된 V. vulnificus 28주를 대상으로 하였다. V. vulnificus 28주 모두에서 vvhA 유전자가 검출되고 good identification 이상의 생화학 동정 결과를 나타내어 V. vulnificus임이 확인되었다. 실험에 사용된 V. vulnificus는 tetracycline과 chloramphenicol 항생제에 감수성을 나타내어 환자 발생 시 이 두 종류의 항생제가 치료에 용이할 것으로 판단되었다. V. cholerae NCCP 13589와 V. parahemolyticus NCCP 11143 표준균주 모두 매실 추출물 2.5%와 5%에서 생육 저지환을 나타내었다. 또한 V. vulnificus 분리균주와 V. vulnificus NCCP 11135 표준균주 모두 매실 추출물 1.25%, 2.5%, 5%에서 생육 저지환을 나타내어 V. cholerae와 V. parahemolyticus에 비해 V. vulnificus에 대한 매실 추출물의 항균활성이 높게 나타났다. V. vulnificus에 대한 매실 추출액 최소살균농도가 1.6%로 나타나 수산물 중 V. vulnificus 생육억제에 매실 추출물이 매우 유용할 것으로 판단되었다. 수산물과 관련된 비브리오균 중 패혈증을 일으키는 V. vulnificus균은 국내에서 매년 지속적으로 환자와 사망자를 발생시키고 있다. 수산물 생식 시 발생할 수 있는 식중독을 예방하기 위한 매실 추출물 활용연구가 필요한 것으로 판단된다.

Keywords

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