Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Ferment Red Ginseng Suppresses the Expression of Matrix Metalloproteinases in UVA-irradiated Human Dermal Fibroblast Cells

발효홍삼의 인간진피섬유모세포에서 UVA로 유도한 염증 및 기질단백분해효소 발현 억제 효능

  • Lee, Keun-Hyeun (Hemin Traditional Korean Medical Clinic) ;
  • Jeong, Seung-Il (Jeonju AgroBio-Materials Institute(JAMI)) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Korean Medicine, Woosuk University) ;
  • Shin, Sang Woo (Division of Applied Medicine, School of Korean Medicine Pusan National University) ;
  • Jeong, Han-Sol (Division of Applied Medicine, School of Korean Medicine Pusan National University)
  • 이근현 (혜민한의원) ;
  • 정승일 ((재) 전주농생명소재연구원) ;
  • 이창현 (우석대학교 한의과대학 해부학교실) ;
  • 신상우 (부산대학교 한의학전문대학원 응용의학부) ;
  • 정한솔 (부산대학교 한의학전문대학원 응용의학부)
  • Received : 2017.03.15
  • Accepted : 2017.04.20
  • Published : 2017.04.25
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Abstract

Prolonged exposure to solar ultraviolet A (UVA) radiation has been known to cause premature skin aging (photo-aging). UVA radiation generates ROS thereby induce degenerative changes of skin such as degradation of dermal collagen, elastic fibers. Matrix metalloproteinases (MMPs), the proteolytic enzymes have been implicated as a major player in the development of UVA-induced photo-aging. Many studies have been conducted to block the harmful effects of UV radiation on the skin. Recently, we are interested in the availability of fermented red ginseng (FRG) as natural matrix metalloproteinases inhibitors (MMPIs). The efficacy difference between red ginseng and FRG has been compared. Both RG and FRG have no cytotoxic effects below the concentration of $300{\mu}g/ml$. Human dermal fibroblasts (HDFs) were pretreated with FRG or RG for 24h, followed by irradiation of UVA. Then, we measured the intracellular ROS production and the expression of MMP, $IL-1{\beta}$ at the mRNA level. We also examined the intracellular localization of $NF-{\kappa}B$ and MMP-9 on the FRG or RG treated and UVA-irradiated HDFs. FRG decreased the intracellular ROS production elicited by UVA. In addition, FRG decreased the mRNA expression of MMP-3, MMP-9, and $IL-1{\beta}$ more efficiently than RG. Furthermore, FRG suppressed the nuclear localization of $NF-{\kappa}B$, and the expression of MMP-9. Taken together, our results suggest that FRG is promising agents to prevent UVA-induced photo-aging by suppressing MMP expression and inflammation.

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References

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