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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of Optical Fiber Glucose and Lactate Biosensors for Bioprocess Monitoring

생물공정 모니터링을 위한 광섬유 포도당 및 젖산 센서의 개발

  • Jung, Chang Hwan (School of Chemical Engineering, Research Center for Biophotonics, Chonnam National University) ;
  • Sohn, Ok-Jae (School of Chemical Engineering, Research Center for Biophotonics, Chonnam National University) ;
  • Rhee, Jong Il (School of Chemical Engineering, Research Center for Biophotonics, Chonnam National University)
  • 정창환 (전남대학교 공과대학 화학공학부) ;
  • 손옥재 (전남대학교 공과대학 화학공학부) ;
  • 이종일 (전남대학교 공과대학 화학공학부)
  • Received : 2017.01.23
  • Accepted : 2017.03.23
  • Published : 2017.03.31
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Abstract

In this work the optical fiber glucose and lactate biosensors were developed by using fluorescent dye and enzyme immobilized on the end tip of an optical fiber. 3-Glycidyloxypropyl)methyldiethoxysilane (GPTMS), (3-Aminopropyl) trimethoxysilane (APTMS) and Methyltrimethoxysilane (MTMS) were used to immobilize glucose oxidase (GOD), lactate oxidase (LOD) and ruthenium(II) complex (tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II), $Ru(dpp)_3^{2+}$) as oxygen sensitive fluorescent dye. MTMS sol-gel was an excellent supporting material for the immobilization of $Ru(dpp)_3^{2+}$, GOD, and LOD on the optical fiber. Storage stability of the optical fiber glucose sensor was kept constant over 20 days, while the optical fiber lactate sensor had constant storage stability over 17 days. The optical fiber glucose and lactate biosensors also maintained good operational stability for 20 hours and 14 hours, respectively. The activities of the immobilized enzymes were most excellent at pH 7 and at $25^{\circ}C$. On-line monitoring of glucose and lactate in a simulated process was performed with the optical fiber glucose and lactate biosensors. On-line monitoring results were agreed with those of off-line data measured with high performance liquid chromatography (HPLC).

Keywords

References

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