Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effects of Black-red Ginseng Extracts on Allergic Inflammation In Vitro and In Vivo

In Vitro 및 In Vivo 알러지성 염증반응에 대한 흑홍삼의 억제효과

  • Yeom, Mijung (Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University) ;
  • Oh, Ju-Young (Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University) ;
  • Lee, Bombi (Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University) ;
  • Hahm, Dae-Hyun (Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University) ;
  • Park, Hi-Joon (Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University)
  • 염미정 (경희대학교 침구경락과학연구센터) ;
  • 오주영 (경희대학교 침구경락과학연구센터) ;
  • 이봄비 (경희대학교 침구경락과학연구센터) ;
  • 함대현 (경희대학교 침구경락과학연구센터) ;
  • 박히준 (경희대학교 침구경락과학연구센터)
  • Received : 2016.12.12
  • Accepted : 2017.03.03
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Black-red ginseng (BRGP) exhibits more potent biological activities than white and red ginseng. However, the effect of BRGP on allergic inflammation has not been studied extensively. In this study, we attempted to evaluate the effects of BRGP on allergic inflammation in vitro and in vivo. The effects of BRGP on pro-inflammatory mediators in phorbol myristate acetate (PMA) and A23187-stimulated human mast cells (HMC-1) and on trimellitic anhydride (TMA)-induced atopic dermatitis-like skin lesions in BALB/c mice were evaluated. BRGP suppressed the expression of $TNF-{\alpha}$, IL-6 and IL-8 from HMC-1 stimulated with PMA and A23187. Furthermore, the oral administration of BRGP markedly significantly reduced apparent severity of atopic dermatitis-like skin lesions, ear swelling, lymph node weight gains and serum IgE levels induced by TMA in mice. BRGP treatment also ameliorated epidermal hyperplasia and infiltration of inflammatory cells including mast cells. Taken together, BRGP possesses significant anti-atopic efficacy, suggesting that it could be used as a potential therapeutic agent for allergic inflammatory diseases, including atopic dermatitis.

Keywords

References

  1. Pawankar, R., Canonica, G. W., Holgate, S. T. and Lockey, R. F. (2012) Allergic diseases and asthma: a major global health concern. Curr. Opin. Allergy Clin. Immunol. 12: 39-41. https://doi.org/10.1097/ACI.0b013e32834ec13b
  2. Choi, W. J., Ko, J. Y., Kim, J. W., Lee, K. H., Park, C. W., Kim, K. H., Kim, M. N., Lee, A. Y., Cho, S. H., Park, Y. L., Choi, J. H., Seo, S. J., Lee, Y. W., Roh, J. Y., Park, Y. M., Kim, D. J. and Ro, Y. S. (2012) Prevalence and risk factors for atopic dermatitis: a cross-sectional study of 6,453 Korean preschool children. Acta Derm. Venereol. 92: 467-471. https://doi.org/10.2340/00015555-1252
  3. Hassan, A. S., Kaelin, U., Braathen, L. R. and Yawalkar, N. (2007) Clinical and immunopathologic findings during treatment of recalcitrant atopic eczema with efalizumab. J. Am. Acad. Dermatol. 56: 217-221. https://doi.org/10.1016/j.jaad.2006.08.025
  4. Holm, J. G., Agner, T., Clausen, M. L. and Thomsen, S. F. (2016) Quality of life and disease severity in patients with atopic dermatitis. J. Eur. Acad. Dermatol. Venereol. 30: 1760-1767. https://doi.org/10.1111/jdv.13689
  5. Leung, D. Y., Hanifin, J. M., Charlesworth, E. N., Li, J. T., Bernstein, I. L., Berger, W. E., Blessing-Moore, J., Fineman, S., Lee, F. E., Nicklas, R. A. and Spector, S. L. (1997) Disease management of atopic dermatitis: a practice parameter. Joint task force on practice parameters, representing the american academy of allergy, asthma and immunology, the american college of allergy, asthma and immunology and the joint council of allergy, asthma and immunology. Work group on atopic dermatitis. Ann. Allergy. Asthma. Immunol. 79: 197-211. https://doi.org/10.1016/S1081-1206(10)63003-7
  6. Kawakami, T., Ando, T., Kimura, M., Wilson, B. S. and Kawakami, Y. (2009) Mast cells in atopic dermatitis. Curr. Opin. Immunol. 21: 666-678. https://doi.org/10.1016/j.coi.2009.09.006
  7. Alenius, H., Laouini, D., Woodward, A., Mizoguchi, E., Bhan, A. K., Castigli, E., Oettgen, H. C. and Geha, R. S. (2002) Mast cells regulate IFN-gamma expression in the skin and circulating IgE levels in allergen-induced skin inflammation. J. Allergy Clin. Immunol. 109: 106-113. https://doi.org/10.1067/mai.2002.120553
  8. Nam, K. Y. (2002) Clinical applications and efficacy of Korean ginseng. J. Ginseng Res. 26: 111-131. https://doi.org/10.5142/JGR.2002.26.3.111
  9. Banerjee, U. and Izquierdo, J. A. (1982) Antistress and antifatigue properties of Panax ginseng: comparison with piracetam. Acta Physiol. Lat. Am. 32: 277-285.
  10. Huo, Y. S., Chen, Y. Z., Yu, Z. Y. and Zhang, P. Y. (1988) The effect of panax ginseng extract (GS) on insulin and corticosteroid receptors. J. Tradit. Chin. Med. 8: 293-295.
  11. Zhang, D., Yasuda, T., Yu, Y., Zheng, P., Kawabata, T., Ma, Y. and Okada, S. (1996) Ginseng extract scavenges hydroxyl radical and protects unsaturated fatty acids from decomposition caused by iron-mediated lipid peroxidation. Free Radic. Biol. Med. 20: 145-150. https://doi.org/10.1016/0891-5849(95)02020-9
  12. Lee, S. M., Bae, B. S., Park, H. W., Ahn, N. G., Cho, B. G., Cho, Y. L. and Kwak, Y. S. (2015) Characterization of korean red ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition. J. Ginseng Res. 39: 384-391. https://doi.org/10.1016/j.jgr.2015.04.009
  13. J.H., L., N., S. G., K., K. E., H.J., S., C.S., M., J., O. H., D.H., K., Roh, S. S., W., C., B., S. Y. and Song, G. Y. (2006) Preparation of black ginseng and its antitumor activity. Kor. J Oriental Physiol. Pathol. 20: 951-956.
  14. Kim, H. J., Lee, J. Y., You, B. R., Kim, H. R., Choi, J. E., Nam, K. Y., Moon, B. D. and Kim, M. R. (2011) Antioxidant activities of ethanol extracts from black ginseng prepared by steaming-drying cycles. J Korean Soc. Food Sci. Nutr. 40: 156-1115162. https://doi.org/10.3746/jkfn.2011.40.2.156
  15. Shin, Y. J., Jang, H. H. and Y., S. G. (2012) Study on antiatopic effects of black ginseng. Kor. J Aesthet. Cosmetol. 10: 91-97.
  16. Yeom, M., Sur, B. J., Park, J., Cho, S. G., Lee, B., Kim, S. T., Kim, K. S., Lee, H. and Hahm, D. H. (2015) Oral administration of Lactobacillus casei variety rhamnosus partially alleviates TMA-induced atopic dermatitis in mice through improving intestinal microbiota. J. Appl. Microbiol. 119: 560-570. https://doi.org/10.1111/jam.12844
  17. Nakae, S., Suto, H., Kakurai, M., Sedgwick, J. D., Tsai, M. and Galli, S. J. (2005) Mast cells enhance T cell activation: Importance of mast cell-derived TNF. Proc. Natl. Acad. Sci. U. S. A. 102: 6467-6472. https://doi.org/10.1073/pnas.0501912102
  18. Heib, V., Becker, M., Taube, C. and Stassen, M. (2008) Advances in the understanding of mast cell function. Br. J. Haematol. 142: 683-694. https://doi.org/10.1111/j.1365-2141.2008.07244.x
  19. Boudreau, R. T., Hoskin, D. W. and Lin, T. J. (2004) Phosphatase inhibition potentiates IL-6 production by mast cells in response to FcepsilonRI-mediated activation: involvement of p38 MAPK. J. Leukoc. Biol. 76: 1075-1081. https://doi.org/10.1189/jlb.1003498
  20. Salamon, P., Shoham, N. G., Gavrieli, R., Wolach, B. and Mekori, Y. A. (2005) Human mast cells release Interleukin-8 and induce neutrophil chemotaxis on contact with activated T cells. Allergy 60: 1316-1319. https://doi.org/10.1111/j.1398-9995.2005.00886.x
  21. Schneider, C., Docke, W. D., Zollner, T. M. and Rose, L. (2009) Chronic mouse model of TMA-induced contact hypersensitivity. J. Invest. Dermatol. 129: 899-907. https://doi.org/10.1038/jid.2008.307
  22. Arts, J. H., Droge, S. C., Bloksma, N. and Kuper, C. F. (1996) Local lymph node activation in rats after dermal application of the sensitizers 2,4-dinitrochlorobenzene and trimellitic anhydride. Food Chem. Toxicol. 34: 55-62. https://doi.org/10.1016/0278-6915(95)00089-5