Parasites, Hosts and Diseases

  • 제55권1호
  • /
  • Pages.21-29
  • /
  • 2017
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  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Development of Urinary Bladder Pre-Neoplasia by Schistosoma haematobium Eggs and Chemical Carcinogen in Mice

  • Chala, Bayissa (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine) ;
  • Choi, Min-Ho (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine) ;
  • Moon, Kyung Chul (Department of Pathology, Seoul National University College of Medicine) ;
  • Kim, Hyung Suk (Department of Urology, Seoul National University College of Medicine) ;
  • Kwak, Cheol (Department of Urology, Seoul National University College of Medicine) ;
  • Hong, Sung-Tae (Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine)
  • 투고 : 2016.10.12
  • 심사 : 2017.02.01
  • 발행 : 2017.02.28
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초록

Schistosoma haematobium is a biocarcinogen of human urinary bladder (UB). The present study investigated developing UB cancer mouse model by injecting S. haematobium eggs into the bladder wall and introduction of chemical carcinogens. Histopathological findings showed mild hyperplasia to epithelial vacuolar change, and high grade dysplasia. Squamous metaplasia was observed in the S. haematobium eggs+NDMA group at week 12 but not in other groups. Immunohistochemistry revealed significantly high expression of Ki-67 in urothelial epithelial cells of the S. haematobium eggs+BBN group at week 20. The qRT-PCR showed high expression of p53 gene in S. haematobium eggs group at week 4 and S. haematobium eggs+BBN group at week 20. E-cadherin and vimentin showed contrasting expression in S. haematobium eggs+BBN group. Such inverse expression of E-cadherin and vimentin may indicate epithelial mesenchymal transition in the UB tissue. In conclusion, S. haematobium eggs and nitrosamines may transform UB cells into squamous metaplasia and dysplasia in correlation with increased expression of Ki-67. Marked decrease in E-cadherin and increase in p53 and vimentin expressions may support the transformation. The present study introduces a promising modified animal model for UB cancer study using S. haematobium eggs.

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