Parasites, Hosts and Diseases

  • 제55권1호
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  • Pages.15-20
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  • 2017
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis

  • Song, Hongyan (Laboratory Animal Center of Nantong University) ;
  • Dong, Ronglian (Jiangsu Provincial Center for Disease Control and Prevention) ;
  • Qiu, Baofeng (Nantong Entry-Exit Inspection and Quarantine Bureau) ;
  • Jing, Jin (Laboratory Animal Center of Nantong University) ;
  • Zhu, Shunxing (Laboratory Animal Center of Nantong University) ;
  • Liu, Chun (Laboratory Animal Center of Nantong University) ;
  • Jiang, Yingmei (Laboratory Animal Center of Nantong University) ;
  • Wu, Liucheng (Laboratory Animal Center of Nantong University) ;
  • Wang, Shengcun (Laboratory Animal Center of Nantong University) ;
  • Miao, Jin (Laboratory Animal Center of Nantong University) ;
  • Shao, Yixiang (Laboratory Animal Center of Nantong University)
  • 투고 : 2016.05.23
  • 심사 : 2016.11.29
  • 발행 : 2017.02.28
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초록

The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

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피인용 문헌

  1. Deaths Due to Mixed Infections with Passalurus ambiguus, Eimeria spp. and Cyniclomyces guttulatus in an Industrial Rabbit Farm in Greece vol.10, pp.6, 2017, https://doi.org/10.3390/pathogens10060756
  2. Pathological changes and antigen localization in the small intestine of rabbits infected with Eimeria magna vol.29, pp.3, 2021, https://doi.org/10.4995/wrs.2021.15254