DOI QR코드

DOI QR Code

Random amplified polymorphic DNA analysis of bacterial pathogens using universal rice primers

  • Monoldorova, Sezim (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Jinsol (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Joon Hee (School of Food Science and Technology, College of Biotechnology & Natural Resource, Chung-Ang University) ;
  • Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University)
  • Received : 2017.01.10
  • Accepted : 2017.03.15
  • Published : 2017.03.30

Abstract

Molecular typing of pathogenic microorganisms is important for epidemiological investigation of infectious disease outbreaks. In this study, we applied Universal Rice Primers (URP) that were originated from repetitive sequences in rice chromosomal DNA to random amplified polymorphic DNA (RAPD) analysis of pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, and Salmonella sp. Of the twelve URP primers examined to date, seven primers (URP-2, -3, -4, -5, -6, -8, and -9) generated reproducible and polymorphic PCR products ranging from 1 to 13 bands. One of them, URP-6 was very effective in differentiating seven E. coli serotypes, seven L. monocytogenes clinical isolates, and eight Salmonella subspecies (ssp.) serovars. The results thus indicate that RAPD analysis using URP primers might be useful in typing bacterial pathogens including E. coli, L. monocytogenes, and Salmonella strains.

Keywords

References

  1. Chang K. 2013. Molecular Epidemiology of Cryptococcus neoformans/ Cryptococcus gattii Complex Isolates from Pigeon Droppings in Korea. J Exp Biomed Sci 19: 213-223.
  2. Chen S1, Li J, Saleh-Lakha S, Allen V, Odumeru J. 2011. Multiple-locus variable number of tandem repeat analysis (MLVA) of Listeria monocytogenes directly in food samples. Int J Food Microbiol 148: 8-14. https://doi.org/10.1016/j.ijfoodmicro.2011.04.014
  3. Franklin RB, Taylor DR, Mills AL. 1999. Characterization of microbial communities using randomly amplified polymorphic DNA (RAPD). J Microbiol Methods 35: 225-235. https://doi.org/10.1016/S0167-7012(99)00003-2
  4. Grover A, Sharma PC. 2016. Development and use of molecular markers: past and present. Crit Rev Biotechnol 36: 290-302. https://doi.org/10.3109/07388551.2014.959891
  5. Jana TK, Singh NK, Koundal KR, Sharma TR. 2005. Genetic differentiation of charcoal rot pathogen, Macrophomina phaseolina, into specific groups using URP-PCR. Can J Microbiol 51: 159-164. https://doi.org/10.1139/w04-122
  6. Kang HW, Park DS, Go SJ, Eun MY. 2002. Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Mol Cells 13: 281-287.
  7. Kang HW, Park DS, Park YJ, Lee BM, Cho SM, Kim KT, Seo GS, Go SJ. 2002. PCR Based Detection of Phellinus linteus using Specific Primers generated from Universal Rice Primer (URP) Derived PCR Polymorphic Band. Mycobiology 30: 202-207. https://doi.org/10.4489/MYCO.2002.30.4.202
  8. Kim DM, Kwon JY, Lim KB, Kim HH. 2007. Genetic Relationship of 12 Taxa of Genus Aquilegia Using URP-RAPD. Flower Res J 15: 58-63.
  9. Kim SH, Cho SW, Hwang SY, Jeon SY, Kim YK. 2010. Molecular Identification Patterns of Clinical Isolates from Korean Patients Infected with Dermatophytes. J Exp Biomed Sci 16: 187-192.
  10. Lim SH, Kim JG, Kang HW. 2009. Novel SCAR primers for specific and sensitive detection of Agrobacterium vitis strains. Microbiol Res. 164: 451-460. https://doi.org/10.1016/j.micres.2007.02.006
  11. Liu Y, Shi X, Li Y, Chen Q, Jiang M, Li W, Qiu Y, Lin Y, Jiang Y, Kan B, Sun Q, Hu Q. 2016. The evaluation and application of multilocus variable number tandem repeat analysis (MLVA) for the molecular epidemiological study of Salmonella enterica subsp. enterica serovar Enteritidis infection. Ann Clin Microbiol Antimicrob 15: 4. https://doi.org/10.1186/s12941-016-0119-3
  12. Pourshafie MR, Saifi M, Mousavi SF, Sedaghat M, Nikbakht GH, Rubino S. 2008. Clonal diversity of Salmonella enterica serotype Typhi isolated from patients with typhoid fever in Tehran. Scand J Infect Dis 40: 18-23. https://doi.org/10.1080/00365540701481529
  13. Ranjbar R, Karami A, Farshad S, Giammanco GM, Mammina C. 2014. Typing methods used in the molecular epidemiology of microbial pathogens: a how-to guide. New Microbiol 37: 1-15.
  14. Reinoso EB, Bettera SG. 2016. Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology. Biochem Mol Biol Educ 44: 391-396. https://doi.org/10.1002/bmb.20957
  15. Soler-Garcia AA, De Jesus AJ, Taylor K, Brown EW. 2014. Differentiation of Salmonella strains from the SARA, SARB and SARC reference collections by using three genes PCR-RFLP and the 2100 Agilent Bioanalyzer. Front Microbiol 5: 417.
  16. Waldron J, Peace CP, Searle IR, Furtado A, Wade N, Findlay I, Graham MW, Carroll BJ. 2002. Randomly Amplified DNA Fingerprinting: A Culmination of DNA Marker Technologies Based on Arbitrarily-Primed PCR Amplification. J Biomed Biotechnol 2: 141-150. https://doi.org/10.1155/S1110724302206026