DOI QR코드

DOI QR Code

Re-evaluation of specimens recorded as Amanita virosa in Korea

한국에 기록된 독우산광대버섯의 재동정

  • Jin, Yong-Ju (Genomics Division, National Institute of Agricultural Sciences) ;
  • Yoo, Ki-Bum (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Ahn, Geum Ran (Department of Microbiology, Dankook University) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University) ;
  • Seok, Soon-Ja (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
  • 진용주 (국립농업과학원 유전체과) ;
  • 유기범 (국립농업과학원 농업미생물과) ;
  • 안금란 (단국대학교 미생물학과) ;
  • 김성환 (단국대학교 미생물학과) ;
  • 석순자 (국립농업과학원 농업미생물과)
  • Received : 2016.11.03
  • Accepted : 2017.02.22
  • Published : 2017.03.01

Abstract

A molecular phylogenetic analysis of 40 fungal specimens that were collected from the Korean peninsula from 2000 to 2015 and recorded as Amanita virosa was performed using internal transcribed spacer sequence data. Results confirmed that Amanita oberwinklerana (14 specimens), Amanita rimosa (5), Amanita pallidorosea (20), and Amanita virosa (1) belong to section Phalloideae of subgenus Lepidella, and the morphological features of these specimens were re-examined. The former three species with deadly poisonous white mushrooms were not yet recorded in Korea. Because of their morphological similarities with A. virosa, they are frequently overlooked or misidentified in the field. All collections were deposited in the Herbarium Conservation Center of the National Institute of Agricultural Sciences.

본 연구는 2000년부터 2015년까지 한국에서 수집되어 Amanita virosa로 동정된 40개 표본의 분자계통학적 종동정을 위해 internal transcribed spacer rDNA 영역의 염기서열을 사용하여 수행하였다. 이번 연구에서 Lepidella 아속의 Phalloideae절에 속하는 Amanita oberwinklerana의 14개 표본, A.cf. rimosa 5개 표본, A. pallidorosea 20개 표본 및 A. virosa 1개 표본을 확인하였고, 그들의 형태학적 특성을 재조사하였다. 백색 계열의 맹독성 버섯에 속하는 위 3종(A. oberwinklerana, A.cf. rimosa, A. pallidorosea)은 아직 국내에는 잘 알려지지 않았지만, 형태학적 특징들이 A. virosa와 매우 유사하여 존재하더라도 오동정되었을 가능성이 높다. 본 연구에 사용된 모든 표본은 국립농업과학원 표본실에 보관되어 있다.

Keywords

References

  1. Bas C. Morphology and subdivision of Amanita and a monograph on its section Lepidella. Persoonia 1969;5:285-573.
  2. Yang ZL. Die Amanita-Arten von Südwestchina. Berlin: J. Cramer; 1997.
  3. Yang ZL. Flora fungorum sinicorum v.27: Amanitaceae. Beijing: Science Press; 2005.
  4. Singer R. The Agaricales in modern taxonomy. Koenigstein: Koeltz Scientific Books; 1986.
  5. Tulloss RE, Yang ZL. Studies in the Amanitaceae [Internet]. Bronx (NY): New York Botanical Garden; 2003. [cited 2015 Oct 05 ]. Available from: http://www.amanitaceae.org.
  6. Largent DL, Thiers HD. How to identify mushrooms to genus II: field identification of genera. Eureka (CA): Mad River Press; 1977.
  7. Zhang L, Yang J, Yang Z. Molecular phylogeny of eastern Asian species of Amanita (Agaricales, Basidiomycota): taxonomic and biogeographic implications. Fungal Divers 2004;17:219-38.
  8. Zhang P, Chen ZH, Xiao B, Tolgor B, Bao HY, Yang ZL. Lethal amanitas of East Asia characterized by morphological and molecular data. Fungal Divers 2010;42:119-33. https://doi.org/10.1007/s13225-010-0018-4
  9. Kim SS, Kim BG, Kim YS, Kim JH, Park YH, Lee YL, Lee JS, Lee JY, Yim JH, Jeong HS, et al. Mushrooms Korean name a unified draft. Kor J Mycol 1978;6:43-55.
  10. Seok SJ, Jin YJ, Kwon SW, Kim YS, Kim WG. A taxonomic study of genus Melanophyllum in Korea. Kor J Mycol 2013;41:205-11. https://doi.org/10.4489/KJM.2013.41.4.205
  11. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987;19:11-5.
  12. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  14. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  16. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003;19:1572-4. https://doi.org/10.1093/bioinformatics/btg180