The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The antimicrobial activity of Daehwanggo against Methicillin-resistant Staphylococcus aureus

대황고(大黃膏)의 메티실린 내성 황색 포도상구균에 대한 항균활성

  • Lee, Sun-Ae (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kong, Ryong (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok-Hua (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Seo, Yun-Soo (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Zhou, Tian (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Sang-A (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Song, Ok-Hee (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Min-Chul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Han, Hyoung-Sun (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Choi, Ji-Na (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
  • 이순애 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 공룡 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 서윤수 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 주전 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 김상아 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 송옥희 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 김민철 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 한형선 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 최지나 (원광대학교 약학대학 한약학과, 원광한약연구소) ;
  • 이영섭 (농촌진흥청 국립원예특작과학원 인삼특작부 인삼특작이용팀) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소)
  • Received : 2017.01.20
  • Accepted : 2017.03.15
  • Published : 2017.03.30
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Abstract

Objectives : Infectious diseases by Methicillin-Resistant Staphylococcus aureus (MRSA) are a growing problem worldwide. Characteristic of MRSA is endlessly mutation to resist antibiotics. Daehwanggo (DHG) is one of the oriental medicine prescriptions contained in Principles and Practice of Eastern Medicine. Daehwanggo was mainly used for external preparation from old times. The purpose of this study is to confirm possibility as supplementary drug of DHG about antibiotics through observation of synergy effect between DHG and commercial antibiotics and to observe restriction on growth of MRSA on any pathway through observation of mechanism. Methods : The minimum inhibitory concentration (MIC) of DHG against MRSA is $500{\sim}2000{\mu}g/m{\ell}$ by broth dilution method. In the checkerboard method, the combinations of DHG with antibiotics has partial synergistic effect or synergy effect and DHG markedly reduced the MICs of the antibiotics oxacillin (OX), gentamicin (GT) against MRSA. In the inhibition of resistance mechanism of DHG against MRSA, the expression of resistance gene and protein about ${\beta}-lactam$ antibiotic was reduced. Also, we observed the effect of DHG about cell membrane permeability against MRSA, and confirmed that DHG suppressed growth of strains by increasing cell membrane permeability. Results : Basis on the result, we speculate that DHG increase antibacterial activity of antibiotics against MRSA by changing the structure of cell wall of MRSA. Conclusions : These data suggest that Daehwanggo possesses possibility as supplementary drug about antibiotics against MRSA.

Keywords

References

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