대한수의학회지 (Korean Journal of Veterinary Research)

  • 제57권1호
  • /
  • Pages.1-7
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  • 2017
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Fucoidan attenuates 6-hydroxydopamine-induced neurotoxicity by exerting anti-oxidative and anti-apoptotic actions in SH-SY5Y cells

  • Kim, Myung-Hwan (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Namgoong, Hoon (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Jung, Bae-Dong (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Kwon, Myung-Sang (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Choi, Yeon-Shik (Department of Laboratory Animal Science, Korea Biopolytechnic College) ;
  • Shin, Taekyun (College of Veterinary Medicine, Jeju National University) ;
  • Kim, Hyoung-Chun (College of Pharmacy, Kangwon National University) ;
  • Wie, Myung-Bok (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2016.10.11
  • 심사 : 2016.12.14
  • 발행 : 2017.03.31
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초록

Parkinson's disease (PD) is an irreversible neurological disorder with related locomotor dysfunction and is characterized by the selective loss of nigral neurons. PD can be experimentally induced by 6-hydroxydopamine (6-OHDA). It has been reported that reactive oxygen species, which deplete endogenous glutathione (GSH) levels, may play important roles in the dopaminergic cell death characteristic of PD. Fucoidan, a sulfated algal polysaccharide, exhibits anti-inflammatory and anti-oxidant actions. In this study, we investigated whether fucoidan can protect against 6-OHDA-mediated cytotoxicity in SH-SY5Y cells. Cytotoxicity was evaluated by using MTT and LDH assays. Fucoidan alleviated cell damage evoked by 6-OHDA dose-dependently. Fucoidan reduced the number of apoptotic nuclei and the extent of annexin-V-associated apoptosis, as revealed by DAPI staining and flow cytometry. Elevation of lipid peroxidation and caspase-3/7 activities induced by 6-OHDA was attenuated by fucoidan, which also protected against cytotoxicity evoked by buthionine-sulfoximine-mediated GSH depletion. Reduction in the glutathione/glutathione disulfide ratio induced by 6-OHDA was reversed by fucoidan, which also inhibited 6-OHDA-induced disruption of mitochondrial membrane potential. The results indicate that fucoidan may have protective action against 6-OHDA-mediated neurotoxicity by modulating oxidative injury and apoptosis through GSH depletion.

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참고문헌

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피인용 문헌

  1. Natural Products and Their Bioactive Compounds: Neuroprotective Potentials against Neurodegenerative Diseases vol.2020, pp.None, 2020, https://doi.org/10.1155/2020/6565396