Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Stress-Responsive and Host-Oriented Role of Nonribosomal Peptide Synthetases in an Entomopathogenic Fungus, Beauveria bassiana

  • Liu, Hang (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Xie, Linan (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Wang, Jing (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Guo, Qiannan (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Yang, Shengnan (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Liang, Pei (Department of Entomology, China Agricultural University) ;
  • Wang, Chengshu (Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) ;
  • Lin, Min (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Xu, Yuquan (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Liwen (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)
  • Received : 2016.06.27
  • Accepted : 2016.11.13
  • Published : 2017.03.28
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Abstract

Beauveria bassiana infects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in B. bassiana remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of B. bassiana ARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth (Plutella xylostella (Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.

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