Journal of Microbiology and Biotechnology

  • 제27권3호
  • /
  • Pages.417-428
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  • 2017
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Significance of Viable but Nonculturable Escherichia coli: Induction, Detection, and Control

  • Ding, Tian (Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University) ;
  • Suo, Yuanjie (Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University) ;
  • Xiang, Qisen (College of Food and Biological Engineering, Zhengzhou University of Light Industry) ;
  • Zhao, Xihong (Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology) ;
  • Chen, Shiguo (Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University) ;
  • Ye, Xingqian (Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University) ;
  • Liu, Donghong (Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University)
  • 투고 : 2016.10.04
  • 심사 : 2016.11.24
  • 발행 : 2017.03.28
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초록

Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods, posing a significant and potential health risk. Therefore, controlling VBNC bacteria in food processing and the environment is of great importance. As the typical one of the gram-negatives, Escherichia coli (E. coli) is a widespread foodborne and waterborne pathogenic bacterium and is able to enter into a VBNC state in extreme conditions (similar to the other gram-negative bacteria), including inducing factors and resuscitation stimulus. VBNC E. coli has the ability to recover both culturability and pathogenicity, which may bring potential health risk. This review describes the concrete factors (nonthermal treatment, chemical agents, and environmental factors) that induce E. coli into the VBNC state, the condition or stimulus required for resuscitation of VBNC E. coli, and the methods for detecting VBNC E. coli. Furthermore, the mechanism of genes and proteins involved in the VBNC E. coli is also discussed in this review.

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  2. Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens vol.8, pp.None, 2017, https://doi.org/10.3389/fmicb.2017.00580
  3. Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection vol.90, pp.15, 2017, https://doi.org/10.1021/acs.analchem.8b02096
  4. Study the Features of 57 Confirmed CRISPR Loci in 38 Strains of Staphylococcus aureus vol.9, pp.None, 2017, https://doi.org/10.3389/fmicb.2018.01591
  5. Survey of verotoxin-producing Escherichia coli and faecal coliforms in beef carcasses destined for export at slaughterhouses in Brazil vol.38, pp.1, 2017, https://doi.org/10.1590/1678-457x.37816
  6. Indoor air purification by dielectric barrier discharge combined with ionic wind: physical and microbiological investigations vol.51, pp.16, 2018, https://doi.org/10.1088/1361-6463/aab48b
  7. Comparison of conventional plating, PMA-qPCR, and flow cytometry for the determination of viable enterotoxigenic Escherichia coli along a gastrointestinal in vitro model vol.102, pp.22, 2017, https://doi.org/10.1007/s00253-018-9380-z
  8. The Association of Cell Division Regulated by DicC With the Formation of Viable but Non-culturable Escherichia coli O157:H7 vol.10, pp.None, 2017, https://doi.org/10.3389/fmicb.2019.02850
  9. Metagenomic Profiling of Microbial Pathogens in the Little Bighorn River, Montana vol.16, pp.7, 2017, https://doi.org/10.3390/ijerph16071097
  10. EmPis-1L, an Effective Antimicrobial Peptide Against the Antibiotic-Resistant VBNC State Cells of Pathogenic Bacteria vol.11, pp.2, 2017, https://doi.org/10.1007/s12602-018-9446-3
  11. Induction, detection, formation, and resuscitation of viable but non‐culturable state microorganisms vol.19, pp.1, 2017, https://doi.org/10.1111/1541-4337.12513
  12. Evaluation by Flow Cytometry of Escherichia coli Viability in Lettuce after Disinfection vol.9, pp.1, 2020, https://doi.org/10.3390/antibiotics9010014
  13. Investigation into the Physiological State of Heat Stressed Escherichia coli Used in the Evaluation Testing of an Intrinsic Fluorescence-Based RMM. vol.25, pp.2, 2017, https://doi.org/10.4265/bio.25.91
  14. The fate of cold‐stressed or TETRACYCLINE‐RESISTANTVibrio spp. in precooked shrimp during frozen storage vol.40, pp.4, 2017, https://doi.org/10.1111/jfs.12798
  15. Viable but non culturable state and expression of pathogenic genes of ESCHERICHIA COLI O157 : H7 in salted silver carp vol.40, pp.5, 2017, https://doi.org/10.1111/jfs.12843
  16. Effects of quorum sensing on the biofilm formation and viable but non-culturable state vol.137, pp.None, 2017, https://doi.org/10.1016/j.foodres.2020.109742
  17. Endophytic Fungi: From Symbiosis to Secondary Metabolite Communications or Vice Versa? vol.12, pp.None, 2017, https://doi.org/10.3389/fpls.2021.791033
  18. How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria vol.10, pp.2, 2017, https://doi.org/10.3390/antibiotics10020115
  19. Development of a New Predictive index (Bathing Water Quality Index, BWQI) Based on Escherichia coli Physiological States for Bathing Waters Monitoring vol.9, pp.2, 2021, https://doi.org/10.3390/jmse9020120
  20. The diagnostic tools for viable but nonculturable pathogens in the food industry: Current status and future prospects vol.20, pp.2, 2021, https://doi.org/10.1111/1541-4337.12695
  21. Survival of escherichia coli in Water Microcosm Study and Rethinking its Use as Indicator vol.90, pp.2, 2017, https://doi.org/10.1134/s0026261721020107