Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Micro-threads of Cross-linked Hyaluronic Acid Hydrogel using a Microfluidic Chip

미세 유체 칩 기반의 히알루론산 미세 실의 제작

  • Lee, Yun-Kyung (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University) ;
  • Lee, Kwang-Ho (Department of Advanced Materials Science and Engineering, College of Engineering, Kangwon National University)
  • 이윤경 (강원대학교 공과대학 신소재공학과) ;
  • 이광호 (강원대학교 공과대학 신소재공학과)
  • Received : 2017.02.16
  • Accepted : 2017.03.05
  • Published : 2017.02.28
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Abstract

The successful synthesis of hyaluronic acid micro-threads is very promising approach for the broad application in tissue engineering such as dermal fillers. Because hyaluronic acid has the excellent biocompatibility and ability to maintain the moisture of up to several hundred times its own weight. In order to generate the hyaluronic acid micro-threads in microfluidic system, we employed two-phase flow microfluidic chip to make a rapid synthesis of the hyaluronic acid hydrogel. Hyaluronic acid was mixed with 0.02N NaOH solution and 1, 4-Butanediol diglycidyl ether (BDDE) solution and then injected into core channel. The ethanol was used for the 3-dimensional micro-thread formation in sheath channel. We manipulated the diameter of HA micro-threads using controlling of flow rates in microfluidic chip, and showed the feasibility of immobilization in HA micro-threads with florescent substances. Also, the generated HA micro-threads were evaluated and showed the suitable properties with tensile strength, bending property, and swelling profiles for dermal fillers. As a result, we suggested an innovative method for microfluidic chip-based HA micro-threads which could safely be applied as dermal filler in tissue engineering.

Keywords

References

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