Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Evaluation of Anti-oxidant, Anti-microbial and Anti-thrombosis Activities of Fruit, Seed and Pomace of Schizandra chinensis Baillon

오미자 열매, 씨, 착즙 후 박의 항산화, 항균 및 항혈전 활성 평가

  • Kim, Mi-Sun (Department of Food and Nutrition, Andong National University) ;
  • Sung, Hwa-Jung (Department of Food and Nutrition, Andong National University) ;
  • Park, Jong-Yi (Gyeongbuk Institute For Bio-Industry) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • Received : 2016.10.07
  • Accepted : 2016.10.12
  • Published : 2017.02.28
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Abstract

In this study, for the efficient use of the byproduct of the omija (Schizandra chinensis Baillon: SC) processing industry, the ethanol extracts of the fruit (F), seed (S), and pomace (P) of SC were prepared, and their useful bioactivities were evaluated. For F-SC, S-SC, and P-SC, the extraction yields were 28.3%, 22.1%, and 7.2%, respectively, and the polyphenol contents were 8.81, 37.22, and 9.20 mg/g, respectively. The total flavonoid content in P-SC (4.31 mg/g) was 3.5-fold higher than that in F-SC (0.76 mg/g). In an antioxidation activity assay, P-SC showed stronger radical scavenging activities against DPPH anion, ABTS cation, and nitrite and stronger reducing power activities than the other extracts. The calculated concentration required for 50% radical scavenging activity, $RC_{50}s$, of P-SC for DPPH anion, ABTS cation, and nitrite was 226.2, 192.5, and $92.5{\mu}g/ml$, respectively. In an antimicrobial activity assay, F-SC, S-SC, and P-SC showed similarly strong growth inhibitions against Bacillus subtilis and P. vulgaris at a concentration of 0.5 mg/disc. F-SC and P-SC showed 15-fold extended time in thrombin, prothrombin, and activated partial thromboplastin time assays at a concentration of 5 mg/ml. The anticoagulation activity of P-SC (2.5 mg/ml) was comparable to that of aspirin (1.5 mg/ml). Furthermore, F-SC and S-SC showed very good platelet aggregation inhibitory activities. F-SC, S-SC, and P-SC did not show significant hemolysis against human red blood cell up to a concentration of 0.5 mg/ml. These results suggest that S-SC and P-SC, both of which are byproducts of the omija processing industry, show strong potential as novel antioxidant, antimicrobial, and antithrombosis agents.

본 연구에서는 오미자 가공산업의 부산물 이용을 위해, 오미자 열매, 씨 및 착즙 후의 박의 에탄올 추출물을 각각 조제하고, 이들의 유용생리활성을 평가하였다. 오미자 열매, 씨 및 박의 추출 수율은 각각 28.3, 22.1 및 7.2%로 나타났으며, 추출물들의 총 폴리페놀 함량은 각각 8.81, 37.22 및 9.20 mg/g로 나타났다. 총 플라보노이드 함량 분석 결과 박(4.31 mg/g)>씨(1.25 mg/g)>열매(0.76 mg/g) 추출물 순으로 나타났다. 항산화 활성 평가 결과, 다양한 radical (DPPH 음이온, ABTS 양이온 및 nitrite) 소거능 및 환원력 평가에서 박>씨>열매 추출물 순으로 항산화능이 나타났으며, 오미자 박 추출물의 DPPH 양이온, ABTS 음이온 및 nitrite 소거능에 대한 $RC_{50}$는 각각 226.2, 192.5 및 $92.5{\mu}g/ml$로 계산되었다. 항균 활성 평가 결과, 오미자 열매, 씨 및 박 추출물(0.5 mg/disc)은 B subtilis 및 P. vulgaris 균에 대한 강한 항균 활성을 나타내었다. 혈액응고저해 활성의 경우, 오미자 열매 및 박 추출물은 5 mg/ml 농도에서 TT, PT 및 aPTT를 모두 무첨가구에 비해 15배 이상 연장시켰으며, 박 추출물(2.5 mg/ml)은 aspirin (1.5 mg/ml)과 유사한 혈액응고 저해를 나타내었다. 혈소판응집 저해 활성 평가 결과, 열매 추출물과 씨 추출물(0.25 mg/ml)은 동일농도의 aspirin 보다 우수한 혈소판 응집저해 활성을 나타내었다. 상기의 오미자 시료들은 0.5 mg/ml 농도까지 유의적인 용혈활성이 나타나지 않았다. 본 연구 결과는 오미자 가공산업에서 발생하는 오미자 씨와 착즙 후의 박을 이용한 항산화, 항균, 항혈전 기능성 소재 개발이 가능함을 제시하고 있다.

Keywords

References

  1. Ahn, S. M., Ryu, H. Y., Kang, D. K., Jung, I. C. and Sohn, H. Y. 2009. Antimicrobial and antioxidant activity of the fruit of Prunus avium L. Kor. J. Microbiol. Biotechnol. 36, 195-200.
  2. Bijak, M., Nowak, P., Bobrowiecka, M., Ponczek, M. B., Zbikowska, H. and Wacowicz, B. 2012. Protective effects of (-)-epicatechin against nitrative modifications of fibrinogen. Thrombosis Res. 130, 123-128.
  3. Caichompoo, W., Zhang, Q. Y., Hou, T. T., Gao, H. J., Qin, L. P. and Zhou, X. J. 2009. Optimization of extraction and purification of active fractions from Schisandra chinensis (Turcz.) and its osteoblastic proliferation stimulating activity. Phytother Res. 23, 289-292. https://doi.org/10.1002/ptr.2585
  4. Chen, H., Qi, X., He, C., Yin, Z., Fan, D. and Han, G. 2013. Coagulation imbalance may not contribute to the development of portal vein thrombosis in patients with cirrhosis. Thrombosis Res. 131, 173-177. https://doi.org/10.1016/j.thromres.2012.11.003
  5. Cho, E. K., Cho, H. E. and Choi, Y. J. 2010. Inhibitory effects of angiotensin converting enzyme and ${\alpha}$-glucosidase, and alcohol metabolizing activity of fermented omija (Schizandra chinensis Baillon) beverage. J. Kor. Soc. Food Sci. Nutr. 39, 655-661. https://doi.org/10.3746/jkfn.2010.39.5.655
  6. Choi, S. R., Kim, C. S., Kim, J. Y., You, D. H., Kim, J. M., Kim, Y. S., Song, E. J., Kim, Y. G., Ahn, Y. S. and Choi, D. G. 2011. Changes of antioxidant activity and lignan contents in Schisandra chinensis by harvesting times. Kor. J. Med. Crop. Sci. 19, 414-420. https://doi.org/10.7783/KJMCS.2011.19.6.414
  7. Choi, Y. W., Takamatsu, S., Khan, S. I., Srinivas, P. V., Ferreira, D., Zhao, J. and Khan, I. A. 2006. Schisandrene, a dibenzocyclooctadien lignin from Schisandra chinensis: structure antioxidant activity relationships of dibenzocyclooctadien lignans. J. Nat. Prod. 69, 356-359. https://doi.org/10.1021/np0503707
  8. Chung, K. H., Lee, S. H., Lee, Y. C. and Kim, J. T. 2001. Antimicrobial activity of Omija (Schizandra chinensis) extracts. J. Kor. Soc. Food Sci. Nutr. 30, 127-132.
  9. Deng, X., Chen, X., Cheng, W., Shen, Z. and Bi, K. 2008. Simultaneous LC-MS quantification of 15 lignans in Schisandra chinensis (Turcz.) Baill. fruit. Chromatographia 67, 559-566. https://doi.org/10.1365/s10337-008-0589-3
  10. Guo, L. Y., Hung, T. M., Bae, K. H., Shin, E. M., Zhou, H. Y., Hong, Y. N., Kang, S. S., Kim, H. P. and Kim, Y. S. 2008. Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill. Eur. J. Pharmacol. 591, 293-299. https://doi.org/10.1016/j.ejphar.2008.06.074
  11. Hyun, K. H., Kim, H. J. and Jeong, H. C. 2002. A study of determining chemical composition of Schizandra chinensis. Kor. J. Plan. Res. 15, 1-7.
  12. Jeon, Y. H., Kil, J. H., Lim, S. M., Kim, M. H. and Kim, M. R. 2008. Analysis of antioxidative activity and antimutagenic effect of ethanol extract from Schizandra chinensis Baillon. J. East Asian Soc. Diet. Life 18, 746-752.
  13. Jo, S. H., Ha, K. S., Moon, K. S., Lee, O. H., Jang, H. D. and Kwon, Y. I. 2011. In vitro and in vivo anti-hyperglycemic effects of omija (Schizandra chinensis) fruit. Int. J. Mol. Sci. 12, 1359-1370. https://doi.org/10.3390/ijms12021359
  14. Jung, G. T., Ju, I. O., Choi, C. S. and Hong, J. S. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis (Omija) seed. Kor. J. Food Sci. Technol. 32, 928-935.
  15. Kim, K. S., Park, C. G., Ryu, S. N., Bang, J. K. and Lee, B. H. 2000. Schizandrin, oil compounds and their extraction yield in fruits of Schizandra chinensis Baillon. Kor. J. Crop. Sci. 45, 158-162.
  16. Kim, M. G., Lee, C. H. and Lee, H. S. 2010. Anti-platelet aggregation activity of lignans isolated from Schisandra chinensis fruit. J. Kor. Soc. Appl. Biol. Chem. 53, 740-745. https://doi.org/10.3839/jksabc.2010.112
  17. Kim, M. S., Seong, H. J. and Sohn, H. Y. 2016. In-vitro antithrombosis activity of different parts of Sorbus commixta from Ulleung island. J. Life Sci. 26, 289-295. https://doi.org/10.5352/JLS.2016.26.3.289
  18. Kim, Y. J., Lee, Y. G., Choi, Y. W. and Kim, Y. C. 2008. Effects of drying conditions on the profile of volatile terpenoid and color of Shizandra Fruit. J. Life Sci. 18, 1066-1071. https://doi.org/10.5352/JLS.2008.18.8.1066
  19. Kim, Y. S., Kim, Y. S., Kim, S. Y., Whang, J. H. and Suh, H. J. 2008. Application of omija (Schiandra chinensis) and plum (Prunus mume) extracts for the improvement of Kimchi quality. Food Control 19, 662-669. https://doi.org/10.1016/j.foodcont.2007.07.006
  20. Kwon, H. J. and Park, C. S. 2008. Biological activities of extracts from Omija (Schizandra chinensis Baillon). Kor. J. Food Preserv. 15, 587-592.
  21. Lee, H. J., Cho, J. S., Lee, Y. M., Choi, J. Y., Sung, J. H., Chung, H. S. and Moon, K. D. 2015. Quality characteristic of Omija (Schizandra chinensis Baillon) seed oils by roasting conditions and extraction methods. Kor. J. Food Preserv. 22, 845-850. https://doi.org/10.11002/kjfp.2015.22.6.845
  22. Lee, J. S. and Lee, S. W. 1991. Effect of water extracts of endocarps and seeds of omija (Schizandra chinensis Baillon) on alcohol metabolism. J. East Asian Soc. Diet. Life 1, 299-304.
  23. Lee, W. Y., Choi, S. Y., Lee, B. S., Park. J. S., Kim, M. J. and Oh, S. L. 2006. Optimization of extraction conditions from omija (Schizandra chinensis Baillon) by response surface methodology. Kor. J. Food Preserv. 17, 637-643.
  24. Liu, C. J., Zhang, S. Q., Zhang, J. S., Liang, Q. and Li, D. S. 2012. Chemical composition and antioxidant activity of essential oil from berries of Schisandra chinensis (Turcz.) Baill. Nat. Prod. Res. 26, 2199-2203. https://doi.org/10.1080/14786419.2011.636745
  25. Martinez, M., Weisel, J. W. and Ischiropoulos, H. 2013. Functional impact of oxidative posttranslational modifications on fibrinogen and fibrin clots. Free Radic. Biol. Med. 65, 411-418. https://doi.org/10.1016/j.freeradbiomed.2013.06.039
  26. Min, H. Y., Park, E. J., Hong, J. Y., Kang, Y. J., Kim, S. J., Chung, H. J., Woo, E. R., Hung, T. M., Youn, U. J., Kim, Y. S., Kang, S. S., Bae, K. H. and Lee, S. K. 2008 Antiproliferative effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis in human cancer cells. Bioorg. Med. Chem. Lett. 18, 523-526. https://doi.org/10.1016/j.bmcl.2007.11.082
  27. Nomura, M., Nakachiyama, M., Hida, T., Ohtaki, Y., Sudo, K., Aizawa, T., Aburada, M. and Miyamoto, K. I. 1994. Gomisin A, a lignan component of Schizandra fruits, inhibits development of preneoplastic lesions in rat liver by 3'-methyl-4-dimethylamino-azobenzene. Cancer Lett. 76, 11-18 https://doi.org/10.1016/0304-3835(94)90128-7
  28. Park, L. Y., Lee, S. H. and Kim, S. J. 2010. Preparation and sensory characteristics of bread containing Schizandra chinensis Baillon (a traditional Korean medicinal plant). Kor. J. Food Preserv. 17, 637-643.
  29. Ryu, I. H., Kwon, T. O. 2012. The antioxidative effect and ingredients of oil extracted from Schizandra chinensis seed. Kor. J. Med. Crop Sci. 20, 63-71. https://doi.org/10.7783/KJMCS.2012.20.1.063
  30. Ryu, S. N., Kim, K. S., Lee, E. B., Kang, S. S., Kim, J. S., Cheon, S. A. and Lee, B. H. 1998. Acute toxicity of fruit pigment and seed oil of Schizandra chinensis in mice. Kor. J. Intl. Agri. 10, 37-41.
  31. Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol. 299, 152-178.
  32. Sung, K. C. 2011. A study on the pharmaceutical and chemical characteristics and analysis of natural omija extract. J. Kor. Oil Chem. Soc. 28, 290-298.
  33. Sweeney, J. D., Hoerning, L. A., Behrens, A. N., Novak, E. and Swank, R. T. 1990. Thrombocytopenia after desmopressin but absence of in-vitro hypersensitivity to ristocetin. Amer. J. Clin. Path. 93, 522-525. https://doi.org/10.1093/ajcp/93.4.522
  34. Valentina, U., Fabcic, J. and Stampar. F. 2007. Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem. 107, 185-192.
  35. Yang, J. C. 2012. The evaluation on the effectiveness as a cosmetic material of oil extracted from Schizandra chinensis seed J. Kor. Oil Chem. Soc. 29, 231-237.
  36. Yim, S. Y., Lee, Y. J., Lee, Y. K., Jung, S. E., Kim, J. H., Kim, J. E., Kim, H. J., Son, B. G., Park, Y. H., Lee, Y. G., Choi, Y. W. and Hwang, D. Y. 2009. Gomisin N isolated from Schisandra chinensis significantly induces anti-proliferative and pro-apoptotic effects in hepatic carcinoma. Mol. Med. Rep. 2, 725-732.