Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Baicalein Inhibits the Migration and Invasion of B16F10 Mouse Melanoma Cells through Inactivation of the PI3K/Akt Signaling Pathway

  • Choi, Eun-Ok (Department of Food and Nutrition, College of Human Ecology, Pusan National University) ;
  • Cho, Eun-Ju (Department of Food and Nutrition, College of Human Ecology, Pusan National University) ;
  • Jeong, Jin-Woo (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University) ;
  • Park, Cheol (Department of Molecular Biology, College of Natural Sciences and Human Ecology, Dongeui University) ;
  • Hong, Su-Hyun (Department of Biochemistry, Dongeui University College of Korean Medicine) ;
  • Hwang, Hye-Jin (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University) ;
  • Moon, Sung-Kwon (Department of Food and Nutrition, Chung-Ang University) ;
  • Son, Chang Gue (Liver and Immunology Research Center, Daejeon Oriental Hospital of Daejeon University) ;
  • Kim, Wun-Jae (Department of Urology, Chungbuk National University College of Medicine) ;
  • Choi, Yung Hyun (Anti-Aging Research Center & Blue-Bio Industry RIC, Dongeui University)
  • Received : 2016.05.03
  • Accepted : 2016.06.07
  • Published : 2017.03.01
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Abstract

Baicalein, a natural flavonoid obtained from the rhizome of Scutellaria baicalensis Georgi, has been reported to have anticancer activities in several human cancer cell lines. However, its antimetastatic effects and associated mechanisms in melanoma cells have not been extensively studied. The current study examined the effects of baicalein on cell motility and anti-invasive activity using mouse melanoma B16F10 cells. Within the noncytotoxic concentration range, baicalein significantly inhibited the cell motility and invasiveness of B16F10 cells in a concentration-dependent manner. Baicalein also reduced the activity and expression of matrix metalloproteinase (MMP)-2 and -9; however, the levels of tissue inhibitor of metalloproteinase-1 and -2 were concomitantly increased. The inhibitory effects of baicalein on cell motility and invasiveness were found to be associated with its tightening of tight junction (TJ), which was demonstrated by an increase in transepithelial electrical resistance and downregulation of the claudin family of proteins. Additionally, treatment with baicalein markedly reduced the expression levels of lipopolysaccharide-induced phosphorylated Akt and the invasive activity in B16F10 cells. Taken together, these results suggest that baicalein inhibits B16F10 melanoma cell migration and invasion by reducing the expression of MMPs and tightening TJ through the suppression of claudin expression, possibly in association with a suppression of the phosphoinositide 3-kinase/Akt signaling pathway.

Keywords

References

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