Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selection of Potential Virulence Factors Contributing to Streptococcus suis Serotype 2 Penetration into the Blood-Brain Barrier in an In Vitro Co-Culture Model

  • Liu, Hongtao (College of Veterinary Medicine, Jilin University) ;
  • Zhu, Seng (College of Veterinary Medicine, Jilin University) ;
  • Sun, Yingying (College of Veterinary Medicine, Jilin University) ;
  • Li, Na (College of Veterinary Medicine, Jilin University) ;
  • Gu, Jingmin (College of Veterinary Medicine, Jilin University) ;
  • Sun, Changjiang (College of Veterinary Medicine, Jilin University) ;
  • Feng, Xin (College of Veterinary Medicine, Jilin University) ;
  • Han, Wenyu (College of Veterinary Medicine, Jilin University) ;
  • Jiang, Jianxia (Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University) ;
  • Lei, Liancheng (College of Veterinary Medicine, Jilin University)
  • Received : 2016.06.09
  • Accepted : 2016.09.21
  • Published : 2017.01.28
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Abstract

Meningitis caused by Streptococcus suis serotype 2 (S. suis 2) is a great threat to the pig industry and human health. Virulence factors associated with the pathogenesis of meningitis have yet to be clearly defined, even though many potential S. suis 2 virulence factors have been identified. This greatly hinders the progress of S. suis 2 meningitis pathogenesis research. In this study, a co-culture blood-brain barrier (BBB) model was established using primary porcine brain microvascular endothelial cells and astrocytes, and the whole genome library of S. suis 2 was constructed using phage display technology. Finally, a total of 14 potential virulence factors contributing to S. suis 2 adherence to and invasion of the BBB were selected by analyzing the interactions between the phage library and the co-culture model. Twelve of these factors have not been previously reported in meningitis-related research. The data provide valuable insight into the pathogenesis of S. suis 2 meningitis and potential targets for the development of drug therapies.

Keywords

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