Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Bacterial Community Shift during the Startup of a Full-Scale Oxidation Ditch Treating Sewage

  • Chen, Yajun (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University) ;
  • Ye, Lin (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University) ;
  • Zhao, Fuzheng (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University) ;
  • Xiao, Lin (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University) ;
  • Cheng, Shupei (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University) ;
  • Zhang, Xu-Xiang (State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University)
  • Received : 2016.07.04
  • Accepted : 2016.10.03
  • Published : 2017.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

The oxidation ditch (OD) is one of the most widely used processes for treating municipal wastewater. However, the microbial communities in the OD systems have not been well characterized, and little information about the shift of bacterial community during the startup process of the OD systems is available. In this study, we investigated the bacterial community changes during the startup period (over 100 days) of a full-scale OD. The results showed that the bacterial community dramatically changed during the startup period. Similar to the activated sludge samples in other studies, Proteobacteria (accounting for 26.3%-48.4%) was the most dominant bacterial phylum in the OD system, but its relative abundance declined nearly 40% during the startup process. It was also found that Planctomycetes proliferated greatly (from 4.79% to 13.5%) and finally replaced Bacteroidetes as the second abundant phylum in the OD system. Specifically, some bacteria affiliated with genus Flavobacterium exhibited remarkable decreasing trends, whereas bacterial species belonging to the OD1 candidate division and Saprospiraceae family were found to increase during the startup process. Despite of the bacterial community shift, the organic matter, nitrogen, and phosphorus in the effluent were always in low concentrations, suggesting the functional redundancy of the bacterial community. Moreover, by comparing with the bacterial community in other municipal wastewater treatment bioreactors, some potentially novel bacterial species were found to be present in the OD system. Collectively, this study improved our understandings of the bacterial community structure and microbial ecology during the startup of a full-scale wastewater treatment bioreactor.

Keywords

References

  1. Hao X, Doddema HJ, van Groenestijn JW. 1997. Conditions and mechanisms affecting simultaneous nitrification and denitrification in a Pasveer oxidation ditch. Bioresour. Technol. 59: 207-215. https://doi.org/10.1016/S0960-8524(96)00143-5
  2. Peng Y, Hou H, Wang S, Cui Y, Zhiguo Y. 2008. Nitrogen and phosphorus removal in pilot-scale anaerobic-anoxic oxidation ditch system. J. Environ. Sci. 20: 398-403. https://doi.org/10.1016/S1001-0742(08)62070-7
  3. Liu Y, Shi H, Xia L, Shi H, Shen T, Wang Z, et al. 2010. Study of operational conditions of simultaneous nitrification and denitrification in a Carrousel oxidation ditch for domestic wastewater treatment. Bioresour. Technol. 101: 901-906. https://doi.org/10.1016/j.biortech.2009.09.015
  4. Gillot S, Heduit A. 2000. Effect of air flow rate on oxygen transfer in an oxidation ditch equipped with fine bubble diffusers and slow speed mixers. Water Res. 34: 1756-1762. https://doi.org/10.1016/S0043-1354(99)00323-1
  5. Ye L, Shao M-F, Zhang T, Tong AHY, Lok S. 2011. Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing. Water Res. 45: 4390-4398. https://doi.org/10.1016/j.watres.2011.05.028
  6. Zhang T, Shao M-F, Ye L. 2012. 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME J. 6: 1137-1147. https://doi.org/10.1038/ismej.2011.188
  7. Wang X, Wen X, Criddle C, Wells G, Zhang J, Zhao Y. 2010. Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems. J. Environ. Sci. 22: 627-634. https://doi.org/10.1016/S1001-0742(09)60155-8
  8. Johnson DR, Lee TK, Park J, Fenner K, Helbling DE. 2015. The functional and taxonomic richness of wastewater treatment plant microbial communities are associated with each other and with ambient nitrogen and carbon availability. Environ. Microbiol. 17: 4851-4860. https://doi.org/10.1111/1462-2920.12429
  9. Saunders AM, Albertsen M, Vollertsen J, Nielsen PH. 2015. The activated sludge ecosystem contains a core community of abundant organisms. ISME J.
  10. APHA/AWWA/WEF. 2005. Standard Methods for the Examination of Water and Wastewater. American Public Health Association, the American Water Works Association, and the Water Environment Federation, Washington, DC.
  11. Claesson MJ, O'Sullivan O, Wang Q, Nikkila J, Marchesi JR, Smidt H, et al. 2009. Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine. PLoS One 4: e6669. https://doi.org/10.1371/journal.pone.0006669
  12. Sogin ML, Morrison HG, Huber JA, Welch DM, Huse SM, Neal PR, et al. 2006. Microb ial diversity in the deep sea and the underexplored "rare biosphere". Proc. Natl. Acad. Sci. USA 103: 12115-12120. https://doi.org/10.1073/pnas.0605127103
  13. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, et al. 2009. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res. 37: D141-D145. https://doi.org/10.1093/nar/gkn879
  14. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. 2009. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75: 7537-7541. https://doi.org/10.1128/AEM.01541-09
  15. Wang Q, Garrity GM, Tiedje JM, Cole JR. 2007. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl. Environ. Microbiol. 73: 5261-5267. https://doi.org/10.1128/AEM.00062-07
  16. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7: 335-336. https://doi.org/10.1038/nmeth.f.303
  17. Puig S, Van Loosdrecht M, Colprim J, Meijer S. 2008. Data evaluation of full-scale wastewater treatment plants by mass balance. Water Res. 42: 4645-4655. https://doi.org/10.1016/j.watres.2008.08.009
  18. Xia S, Liu J. 2004. An innovative integrated oxidation ditch with vertical circle for domestic wastewater treatment. Process Biochem. 39: 1111-1117. https://doi.org/10.1016/S0032-9592(03)00216-4
  19. Gao S-Y, Peng Y-Z, Wang S-Y, Jun Y. 2006. Novel strategy of nitrogen removal from domestic wastewater using pilot Orbal oxidation ditch. J. Environ. Sci. 18: 833-839. https://doi.org/10.1016/S1001-0742(06)60001-6
  20. Hu M, Wang X, Wen X, Xia Y. 2012. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis. Bioresour. Technol. 117: 72-79. https://doi.org/10.1016/j.biortech.2012.04.061
  21. Blackall LL, Burrell PC, Gwilliam H, Bradford D, Bond PL, Hugenholtz P. 1998. The use of 16S rDNA clone libraries to describe the microbial diversity of activated sludge communities. Water Sci. Technol. 37: 451-454. https://doi.org/10.1016/S0273-1223(98)00261-3
  22. Chouari R, Le Paslier D, Daegelen P, Ginestet P, Weissenbach J, Sghir A. 2003. Molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant. Appl. Environ. Microbiol. 69: 7354-7363. https://doi.org/10.1128/AEM.69.12.7354-7363.2003
  23. Strous M, Fuerst JA, Kramer EH, Logemann S, Muyzer G, van de Pas-Schoonen KT, et al. 1999. Missing lithotroph identified as new planctomycete. Nature 400: 446-449. https://doi.org/10.1038/22749
  24. Chamchoi N, Nitisoravut S. 2007. Anammox enrichment from different conventional sludges. Chemosphere 66: 2225-2232. https://doi.org/10.1016/j.chemosphere.2006.09.036
  25. Feng Q, Han L, Nogi Y, Hong Q, Lv J. 2016. Flavobacterium lutivivi sp. nov., isolated from activated sludge. Int. J. Syst. Evol. Microbiol. 66: 1394-1400. https://doi.org/10.1099/ijsem.0.000892
  26. Basson A, Flemming L, Chenia H. 2008. Evaluation of adherence, hydrophobicity, aggregation, and biofilm development of Flavobacterium johnsoniae-like isolates. Microb. Ecol. 55: 1-14. https://doi.org/10.1007/s00248-007-9245-y
  27. Jo SJ, Kwon H, Jeong S-Y, Lee C-H, Kim TG. 2016. Comparison of microbial communities of activated sludge and membrane biofilm in 10 full-scale membrane bioreactors. Water Res. 101: 214-225. https://doi.org/10.1016/j.watres.2016.05.042
  28. Vandenberg O, Dediste A, Houf K, Ibekwem S, Souayah H, Cadranel S, et al. 2004. Arcobacter species in humans. Emerg. Infect. Dis. 10: 1863-1867. https://doi.org/10.3201/eid1010.040241
  29. Grehn M, von Graevenitz A. 1978. Search for Acinetobacter calcoaceticus sub sp. anitratus: enrichment of fecal samples. J. Clin. Microbiol. 8: 342.
  30. Falk MW, Song K-G, Matiasek MG, Wuertz S. 2009. Microbial community dynamics in replicate membrane bioreactors - natural reproducible fluctuations. Water Res. 43: 842-852. https://doi.org/10.1016/j.watres.2008.11.021
  31. Yadav TC, Khardenavis AA, Kapley A. 2014. Shifts in microbial community in response to dissolved oxygen levels in activated sludge. Bioresour. Technol. 165: 257-264. https://doi.org/10.1016/j.biortech.2014.03.007
  32. Ju F, Guo F, Ye L, Xia Y, Zhang T. 2014. Metagenomic analysis on seasonal microbial variations of activated sludge from a full-scale wastewater treatment plant over 4 years. Environ. Microbiol. Rep. 6: 80-89. https://doi.org/10.1111/1758-2229.12110
  33. Ye C, Yang X, Zhao F-J, Ren L. 2016. The shift of the microbial community in activated sludge with calcium treatment and its implication to sludge settleability. Bioresour. Technol. 207: 11-18. https://doi.org/10.1016/j.biortech.2016.01.135

Cited by

  1. The microbial community in filamentous bulking sludge with the ultra-low sludge loading and long sludge retention time in oxidation ditch vol.9, pp.1, 2017, https://doi.org/10.1038/s41598-019-50086-3
  2. Study on Propellers Distribution and Flow Field in the Oxidation Ditch Based on Two-Phase CFD Model vol.11, pp.12, 2017, https://doi.org/10.3390/w11122506
  3. Assessment of the Dynamics of Microbial Community Associated with Tetraselmis suecica Culture under Different LED Lights Using Next-Generation Sequencing vol.29, pp.12, 2017, https://doi.org/10.4014/jmb.1910.10046