Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of a Bacillus thuringiensis Surface Layer Protein with Cytotoxic Activity against MDA-MB-231 Breast Cancer Cells

  • Rubio, Viviana P. (Universidad Autonoma de Baja California (UABC), Marine Science Faculty) ;
  • Bravo, Alejandra (Universidad Nacional Autonoma de Mexico (UNAM), Biotechnology Institute) ;
  • Olmos, Jorge (Centro de Investigacion Cientifica y de Educacion Superior de Ensenada (CICESE), Department of Marine Biotechnology)
  • Received : 2016.07.08
  • Accepted : 2016.09.30
  • Published : 2017.01.28
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Abstract

In this work, we isolated a surface layer protein (SLP) from a Bacillus thuringiensis (Bt) strain to evaluate it cytotoxic effects against MDA-MB-231 human breast cancer cells. AP11 was selected from a g roup of Bt strains using SLP olig onucleotides developed from Bacillus conserved regions. The AP11 strain was grown in Luria Bertani medium until the late exponential phase; an 86 kDa protein was extracted using 5 M LiCl and identified by liquid chromatography-tandem mass spectrometry. It corresponded to a multispecies SLP highly similar to previously described SLPs in Bt. The MDA-MB-231 breast cancer cells $LC_{50}$ was obtained using $0.25{\mu}g/ml$ of the isolated SLP. HaCat non-cancerous cells presented 90% survival using the same protein concentration. Our data suggest that SLP cytotoxicity against MDA-MB-231 could be induced by an interaction with the CDH11 cell membrane receptor.

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