한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제25권1호
  • /
  • Pages.29-36
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  • 2017
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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겉보리에서 배양한 영지버섯 추출물의 항산화 및 항염증 효능 평가

Evaluation of Anti-Oxidant and Anti-Inflammatory Activities of Ganoderma lucidum Cultured on Hulled Barley

  • 서경희 (건국대학교 응용생화학과) ;
  • 김연화 (건국대학교 응용생화학과) ;
  • 이영민 (건국대학교 응용생화학과) ;
  • ;
  • 박강민 (건국대학교 응용생화학과) ;
  • 박동현 (건국대학교 응용생화학과) ;
  • 김진성 (건국대학교 응용생화학과) ;
  • 임병우 (건국대학교 응용생화학과)
  • Seo, Kyoung Hee (Department of Applied Biochemistry, Konkuk University) ;
  • Kim, Yeon Hwa (Department of Applied Biochemistry, Konkuk University) ;
  • Lee, Young Min (Department of Applied Biochemistry, Konkuk University) ;
  • Ghosh, Mithun (Department of Applied Biochemistry, Konkuk University) ;
  • Park, Kang Min (Department of Applied Biochemistry, Konkuk University) ;
  • Park, Dong Hyun (Department of Applied Biochemistry, Konkuk University) ;
  • Kim, Jin Seong (Department of Applied Biochemistry, Konkuk University) ;
  • Lim, Beong Ou (Department of Applied Biochemistry, Konkuk University)
  • 투고 : 2016.11.15
  • 심사 : 2017.02.07
  • 발행 : 2017.02.28
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초록

Background: Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and anti-inflammatory agents. Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and $Fe^{3+}$ to $Fe^{2+}$ reducing power activity. The ethanol extract of Ganoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treatment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%, even at a concentration of $3,000{\mu}g/m{\ell}$. The ethanol extract inhibited reactive oxygen species (ROS) generation in RAW264.7 cells stimulated with $H_2O_2$, even at low concentrations. In addition, the ethanol extract showed an inhibitory effects on the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells. Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.

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피인용 문헌

  1. Application of Near Infrared Reflectance Spectroscopy for Rapid and Non-Destructive Discrimination of Hulled Barley, Naked Barley, and Wheat Contaminated with Fusarium vol.18, pp.2, 2018, https://doi.org/10.3390/s18010113