Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Identification and characterization of the MYC2 gene in relation to leaf senescence response in hybrid poplar (Populus alba × P. glandulosa)

현사시나무에서 MYC2 유전자의 분리 및 노화 지연에 관한 특성 구명

  • Choi, Hyunmo (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Bae, Eun-Kyung (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Cho, Jin Seong (Department of Plant and Environmental New Resources, Kyung Hee University) ;
  • Lee, Hyoshin (Forest Biotechnology Division, National Institute of Forest Science) ;
  • Choi, Young-Im (Forest Biotechnology Division, National Institute of Forest Science)
  • 최현모 (국립산림과학원 산림생명공학과) ;
  • 배은경 (국립산림과학원 산림생명공학과) ;
  • 조진성 (경희대학교 식물환경신소재공학과) ;
  • 이효신 (국립산림과학원 산림생명공학과) ;
  • 최영임 (국립산림과학원 산림생명공학과)
  • Received : 2017.09.20
  • Accepted : 2017.11.16
  • Published : 2017.12.31
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Abstract

The vegetation period of trees might be prolonged by the delay of the leaf senescence in autumn. Thus, we focused on the generation of senescence-delayed transgenic trees to enhance biomass production. The PagMYC2, a gene containing the basic helix-loop-helix domain, was selected as a candidate for a senescence-delayed transgenic tree. The PagMYC2 gene was specifically induced after treatment with phytohormone jasmonic acid, and upregulated by abiotic stresses such as salinity, osmotic pressure and a low temperature. The constitutive overexpression of the PagMYC2 delayed the leaf senescence and inhibited chlorophyll degradation in the transgenic poplars. Leaf senescence analysis was performed in the leaf tissues of the PagMYC2-over-expression transgenic poplars. The transgenic poplars exhibited higher photochemical efficiency than did a wild type plant under a short-day condition (6 hours light/18 hours darkness) or a low temperature condition ($15^{\circ}C$) that was similar to the weather conditions of autumn. These results suggest that the PagMYC2 is a useful genetic resource to improve biomass production, which is able to sustain growth with senescence-delayed leaves for a long time in autumn.

JA는 병원균과 곤충에 대한 방어기작 뿐만 아니라 식물 노화에도 관여하는 식물 호르몬이다. Basic helix-loop-helix 전사인자인 MYC2는 JA의 신호전달반응의 핵심조절자 역할을 하는 것으로 알려져 있다. 본 연구에서는 현사시나무에서 MYC2 유전자를 분리하고 발현특성을 조사하였으며, 다양한 환경 스트레스에 대한 내성을 갖는 임목을 생산하기 위하여 MYC2를 과발현시킨 현사시나무를 개발하였다. 포트에 식재된 MYC2 과발현 현사시나무는 대조구에 비해 잎 노화 표현형이 지연되는 특징을 보였으며, 엽록소 손실이 적은 것으로 나타났다. 또한 가을의 온도 및 광 주기 조건에서 MYC2 과발현 현사시나무의 광화학 효율을 측정한 결과 대조구보다 높은 특징을 보였다. 따라서 현사시나무의 MYC2 유전자가 낙엽이 지는 가을 동안에도 지속적인 생장을 가능하게 하여 임목의 바이오매스를 증진시키는데 기여할 수 있을 것으로 판단된다.

Keywords

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