Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Multi Sample Array System Based on Pneumatic Valve

공압식 미세밸브를 이용한 다중유체 배열장치 개발

  • Kim, Chul Min (School of Mechanical Engineering, Kyungpook National University) ;
  • Park, Seo Jung (School of Mechanical Engineering, Kyungpook National University) ;
  • Kim, Gyu Man (School of Mechanical Engineering, Kyungpook National University)
  • 김철민 (경북대학교 기계공학부) ;
  • 박서정 (경북대학교 기계공학부) ;
  • 김규만 (경북대학교 기계공학부)
  • Received : 2016.01.12
  • Accepted : 2016.08.18
  • Published : 2017.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

We present a multi-sample array device based on a pneumatic system. Solenoid valves were used to control a micro valve in a pneumatic system. The use of a compressor together with a vacuum pump ensured that one outlet could supply both compression and vacuum pressure. The multi-sample array device was fabricated using conventional photolithography and PDMS casting. The device was composed of a multiplexer, sample array, and rinsing. The multiplexer could control four sample solutions injecting into the sample array chamber. Sample solution not arrayed was removed by DI-water from the rinsing inlet. To prevent trapping of microbubbles in the channel during injection of sample solution into the device, surfactant was added in PDMS solution to serve as a hydrophilic surface treatment. As a result, the device could be used as a sample array for 64 cases, using four samples and three columns of three chambers.

Keywords

References

  1. Kim, H. G., Kim, K. M., Kim, Y. H., Lee, S. H., and Kim, G. M., "Preparation of Monodisperse ENX-Loaded PLGA Microspheres Using a Microfluidic Flow-Focusing Device," Journal of Biobased Materials and Bioenergy, Vol. 7, No. 1, pp. 108-114, 2013. https://doi.org/10.1166/jbmb.2013.1263
  2. Kim, C. M., Park, S. J., and Kim, G. M., “Applications of PLGA Microcarriers Prepared Using Geometrically Passive Breakup on Microfluidic Chip,” Int. J. Precis. Eng. Manuf., Vol. 16, No. 11, pp. 2545-2551, 2015. https://doi.org/10.1007/s12541-015-0326-4
  3. Dang, T.-D., Kim, Y. H., Kim, H. G., and Kim, G. M., “Preparation of Monodisperse PEG Hydrogel Microparticles Using a Microfluidic Flow-Focusing Device,” Journal of Industrial and Engineering Chemistry, Vol. 18, No. 4, pp. 1308-1313, 2012. https://doi.org/10.1016/j.jiec.2012.01.028
  4. Choi, J. H. and Kim, G. M., “Micro-Patterning on Non-Planar Surface Using Flexible Microstencil,” Int. J. Precis. Eng. Manuf., Vol. 12, No. 1, pp. 165-168, 2011. https://doi.org/10.1007/s12541-011-0023-x
  5. Huebner, A., Bratton, D., Whyte, G., Yang, M., Abell, C., et al., “Static Microdroplet Arrays: a Microfluidic Device for Droplet Trapping Incubation and Release for Enzymatic and Cell-Based Assays,” Lab on a Chip, Vol. 9, No. 5, pp. 692-698, 2009. https://doi.org/10.1039/B813709A
  6. Cohen, D. E., Schneider, T., Wang, M., and Chiu, D. T., "Self-Digitization of Sample Volumes," Analytical Chemistry, Vol. 82. No. 13, pp. 5707-5717, 2010. https://doi.org/10.1021/ac100713u
  7. Xia, Y. and Whitesides, G. M., "Soft Lithography," Annual Review of Materials Science, Vol. 28 No. 1, pp. 153-184, 1998. https://doi.org/10.1146/annurev.matsci.28.1.153
  8. Kim, J., Taylor, D., Agrawal, N., Wang, H., Kim, H., et al., “A Programmable Microfluidic Cell Array for Combinatorial Drug Screening,” Lab on a Chip, Vol. 12, No. 10, pp. 1813-1822, 2012. https://doi.org/10.1039/c2lc21202a
  9. Unger, M. A., Chou, H. P., Thorsen, T., Scherer, A., and Quake, S. R., “Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography,” Science, Vol. 288, No. 5463, pp. 113-116, 2000. https://doi.org/10.1126/science.288.5463.113
  10. Sundararajan, N., Kim, D., and Berlin, A. A., “Microfluidic Operations Using Deformable Polymer Membranes Fabricated by Single Layer Soft Lithography,” Lab on a Chip, Vol. 5, No. 3, pp. 350-354, 2005. https://doi.org/10.1039/b500792p
  11. Li, N., Sip, C., and Folch, A., "Microfluidic Chips Controlled with Elastomeric Microvalve Arrays," Journal of Visualized Experiments: JoVE, Vol. 8, 2007.
  12. Huang, S., He, Q., Hu, X., and Chen, H., "Fabrication of Micro Pneumatic Valves with Double-Layer Elastic Poly (Dimethylsiloxane) Membranes in Rigid Poly (Methyl Methacrylate) Microfluidic Chips," Journal of Micromechanics and Microengineering, Vol. 22, No. 8, Paper No. 085008, 2012.
  13. Dy, A. J., Cosmanescu, A., Sluka, J., Glazier, J. A., Stupack, D., et al., "Fabricating Microfluidic Valve Master Molds in SU-8 Photoresist," Journal of Micromechanics and Microengineering, Vol. 24, No. 5, Paper No. 057001, 2014.
  14. Au, A. K., Lai, H., Utela, B. R., and Folch, A., "Microvalves and Micropumps for BioMEMS," Micromachines, Vol. 2, No. 2, pp. 179-220, 2011. https://doi.org/10.3390/mi2020179
  15. Hosokawa, K. and Maeda, R., “A Pneumatically-Actuated Three-Way Microvalve Fabricated with Polydimethylsiloxane Using the Membrane Transfer Technique,” Journal of Micromechanics and Microengineering, Vol. 10, No. 3, pp. 415-420, 2000. https://doi.org/10.1088/0960-1317/10/3/317
  16. Cooksey, G. A., Sip, C. G., and Folch, A., “A Multi-Purpose Microfluidic Perfusion System with Combinatorial Choice of Inputs, Mixtures, Gradient Patterns, and Flow Rates,” Lab on a Chip, Vol. 9, No. 3, pp. 417-426, 2009. https://doi.org/10.1039/B806803H
  17. Yang, W., Nam, Y. G., Lee, B. K., Han, K., Kwon, T. H., et al., "Fabrication of a Hydrophilic Poly (Dimethylsiloxane) Microporous Structure and Its Application to Portable Microfluidic Pump," Japanese Journal of Applied Physics, Vol. 49, No. 6S, 2010.

Cited by

  1. Non-Contact Intraocular Pressure Measurement Method using Relation between Deformed Cornea and Reflected Pneumatic Pressure vol.19, pp.5, 2018, https://doi.org/10.1007/s12541-018-0088-x