The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidative and Antimicrobial Activities of Herbal Complex Extract from Hwangryunhaedok-tang and Essential Oil of Chamaecyparis obtusa

황련해독탕(黃連解毒湯)·편백(扁柏) 정유 복합추출물의 항산화 및 항균활성에 관한 연구

  • Kim, Bo-Ae (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University) ;
  • Park, Shin-Ho (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University) ;
  • Yang, Jae-Chan (Division of Biomedicinal & Cosmetics, College of Sciences & Technology, Mokwon University)
  • 김보애 (목원대학교 테크노과학대학 생의약화장품학부) ;
  • 박신호 (목원대학교 테크노과학대학 생의약화장품학부) ;
  • 양재찬 (목원대학교 테크노과학대학 생의약화장품학부)
  • Received : 2016.12.10
  • Accepted : 2017.01.15
  • Published : 2017.01.30
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Abstract

Objectives : The present study was designed to investigate effects of mixed extracts (9:1, v/v) of Hwangryunhaedoktang, Chamaecyparis obtusa essential oil. We evaluated the antimicrobial and antioxidant activity by manufacturing mixed extracts as the materials for functional medicinal herb cosmetics. Methods : We performed antimicrobial were tested microbes (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, Propionibacterium acnes) by disc diffusion method and measure clear zone. Antioxidant activities were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Results : Antimicrobial activities of mixed extracts against S. epidermideis, P. acnes, S. aureus, E. coli, and Candida. A were $10.9{\pm}3.0mm$, $16.9{\pm}2.0mm$, $9.2{\pm}2.0mm$, $10.3{\pm}1.0mm$, $11.8{\pm}3.0mm$ respectively. The Chamaecyparis obtusa essential oil had the highest antimicrobial activities against S. epidermideis, P. acnes, S. aureus, E. coli, and Candia. A and clear zone of microbes ware $16.7{\pm}3.0mm$, $28.8{\pm}0.2mm$, $15.9{\pm}2.0mm$, $11.5{\pm}1.0mm$, $16.3{\pm}3.0mm$. Hwangryunhaedoktang extract showed antimicrobial activity but only P. acnes and S. aureus The antioxidant activities of the mixed extracts were tested through the evaluation of DPPH radical scavenging activity. The 100 % mixed extracts were found to have 90 % DPPH radical scavenging activity. The mixed extracts was presented similar antioxidant activities compared with that of ascorbic acid. As a result, A mixture extract is expected to have antimicrobial effects and free radical scavenging activity was found. Conclusions : Accordingly, It can be concluded that mixed extracts has the potential to cosmetic material.

Keywords

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