DOI QR코드

DOI QR Code

녹차잎, 뽕잎, 마테잎 추출물이 S. mutans에 미치는 항균효과

Anti-bacteria effect of green tea, mulberry, and mate leaves extracts on S. mutans

  • 김성숙 (건양대학교병원 치과) ;
  • 원지혜 (건양대학교 의과학대학 치위생학과) ;
  • 이고은 (건양대학교 의과학대학 치위생학과) ;
  • 이루리 (건양대학교 의과학대학 치위생학과) ;
  • 이선미 (건양대학교 의과학대학 치위생학과) ;
  • 이진희 (건양대학교 의과학대학 치위생학과) ;
  • 강경희 (건양대학교 의과학대학 치위생학과)
  • Kim, Sung-Sook (Dept. of Dentistry, Konyang University Hospital) ;
  • Won, Ji-Hye (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Go-Eun (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Ru-Ri (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Sun-Mi (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Lee, Jin-Hee (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University) ;
  • Kang, Kyung-hee (Dept. of Dental Hygiene, Collage of Medical Science, Konyang University)
  • 투고 : 2016.11.01
  • 심사 : 2017.01.20
  • 발행 : 2017.01.28

초록

본 연구는 일상에서 흔히 차로 음용되고 있는 녹차잎, 마테잎, 뽕잎이 치아우식원인균으로 알려진 S. mutans에 미치는 항균효과를 알아보고자 하였다. S. mutans를 추출물이 첨가된 배지에 1%씩 접종하고 $37^{\circ}C$에서 6시간과 10시간동안 배양한 후 흡광도 및 세균 집락수를 측정하였다. 녹차잎, 마테잎, 추출물은 0, 0.5, 1.0, 2.0, 4.0%의 농도로 배지에 첨가하였다. S. mutans의 성장억제효과를 확인한 결과, 농도가 높을수록 colony의 수가 현저히 줄어드는 것을 관찰 할 수 있었다. 2% 추출물을 첨가하고 10시간 후 세균 집락수를 측정하였을 때 녹차잎은 99.0%, 뽕잎은 97.1%, 마테잎에서는 89.6%의 높은 성장억제율을 나타내었다.

This study aims to figure out how green tea leaves, mulberry leaves, and mate leaves that are often taken around in drinking tea influence on S. mutans known as dental caries causative bacteria. After vaccinating 1% of S. mutans on the extract added badge and incubating 6 hours and 10 hours in $37^{\circ}C$, it calculated absorbance and bacterial colony number. Extract of green tea leaves and mate leaves were added with 0, 0.5, 1.0, 2.0, and 4.0% of concentration. As a result of growth suppressive effect of S. mutans, it may be observed that colony number significantly decreases in higher concentration. When it calculated bacterial colony number with adding 2% extract after 10 hours, 99.0% for green tea leaves, 97.1% for mulberry leaves, and 89.6% for mate leaves appeared.

키워드

참고문헌

  1. Hahn CL, Falkler WA, Jr, Minah GE, "Microbiological studies of carious dentine from human teeth with irreversible pulpitis." Arch Oral Biol, Vol. 36, No. 2, pp. 147-153 ,1991. https://doi.org/10.1016/0003-9969(91)90077-8
  2. Lee SS, Zhang W, Li Y, "The antimicrobial potential of 14 natural herbal dentifrices: Results of an in vitro diffusion method study." J Am Dent Assoc, Vol 135, pp. 1133-1141 ,2004. https://doi.org/10.14219/jada.archive.2004.0372
  3. Adria FA, Zidan OA. "Natural products for dental caries prevention." J Med Food, Vol. 7, pp. 381-384 ,2004 https://doi.org/10.1089/jmf.2004.7.381
  4. Yoo Hyun Hwang, Jeong Weon Yoon. "The Effects of extracts of Formitopsis pinicola on the dental caries pathogens." Journal of the Korean academy of dental health, Vol. 31, No. 2, pp. 193-204 ,2007.
  5. Eun Soo Sohn, "Proceedings of 2004 KIChE and KSIEC Meeting : Domestic R&D trend analysis of functional food using medical plants." Vol. 8, No. 2, pp. 470-473 ,2003
  6. Da Hong Lee, Hyeon Hee Yu, Su Young Jung, Hea Dalma Moon, Su Min Kim, Byung Hun Jeon, Yong Ouk You, "Anticariogenic Properties of the Ethanol Extract of Tribuli fructus against Streptococcus mutans." Journal of physiology & pathology in Korean Medicine, Vol. .21, No. 5, pp. 1148-1153 ,2007
  7. Jong-Soo MOK, Ki-Cheol SONG, Nack-JOONG CHOI and Ho-Shik YANG. "Antibacterial Effect of Cinnamon (Cinnamomum cassia) Bark Extract against Fish Pathogenic Bacteria." J. Korean Fish, Soc. Vol. 34, No. 5, pp. 545-549 ,2001.
  8. Kwang Hak Bae, Byung Jin Lee, Yun Kyung Jang, Byung Ryeul Lee, Won Jae Lee, Duk Soo Chang, Hyock Soo Moon, Dai Il Paik, Johng Bai Kim. "The effect of mouthrinse products containing sodium fluoride, cetylpyridinium chloride(CPC), pine leaf extracts and green tea extracts on the plaque, gingivitis, dental caries and halitosis." J Korean Acad Dent Health, Vol. 25, No. 1, pp. 51-59 ,2001.
  9. Rousff RL, Martin SF, Youtsey CO: Quantitative survey of narirutin, naringin, hesperidin and neohesperidin in citrus, J Agric Food Chem, 35: 1027-1030, 1987. https://doi.org/10.1021/jf00078a040
  10. Mosci F, Perito S, Bassa S, Capuano A. "The role of Stretococcus mutans in human caries." Minerva Stomatol, Vol. 39, pp. 413-429 ,1990.
  11. Hee-Don Choi, Yoon-Jeoung Koh, In-Wook Choi, Yun-Sook kim and Yong-kon Park, "Anticariogenic Activity and Glucosyltransferase Inhibitory Effects of Extracts from Pine Needle and Twig." KOREAN J. FOOD SCL TECHNOL, Vol. 39, No. 3, pp. 336-341 ,2007.
  12. Lim SH, Seo JS, Yoon YJ, Kim KW, Yoo SY, Kim HS, Kook JK, Lee BR, Cha JH, Park JY, "Ettect of Leaf-Extract from Camellia sinensis and Seed-Extract from Casia tora on Viability of Mutans Streptococci isolated from the interface between orthodontic brackets and tooth surfaces." The Korean Journal of Orthod, Vol. 33, No. 5, pp. 381-389 ,2003.
  13. Kwang Jin Son, Soon Aeh Lee, Gee Don Lee, Young Sam Kim, Jae Gyu Jeon, Kee Wan Chang, "Effects of crude Sanguisorba officinalis L. extract on the growth and the adherence to hydroxyapatite beads of mutans streptococci." J Korean Acad Dent Health, Vol. 28, No. 1, pp. 97-104 ,2004.
  14. Heui Sam Lee, Sun Yeou Kim, Ho Jung Jeon, Sang Duk Lee, Jae Yoo Moon, Ae Jung Kim, Won Chu Lee, Kang Sun Ryu, "Growth inhibitory Effect of Clostridium perfringens for Catechins sparated from Mulberry Leaf." Korean J Seric, Vol. 42, pp. 6-9 ,2000.
  15. Hirasawa M, Takada K, Otake S, "Inhibition of acid production in dental plaque bacteria by green tea catechins." Caries Res, Vol. 40, pp. 265-270 ,2006. https://doi.org/10.1159/000092236
  16. Sun-Yeou Kim, Won-Chu Lee, Hyun-Bok Kim, Ae-Jung Kim, Soon-Kyung Kim, "Antihyperlipidemic Effects of Methanol Extracts from Mulberry Leaves in Cholesterol - Induced Hyperlipidemia Rats." Korean Journal of Food Science, Vol. 27, pp. 1217-1222 ,1999.
  17. Hattori M, Kusumoto IT, Namba T, Ishigami, T, Hara Y, "Effect of tea polyphenols on glucan synthesis by glucosyltransferase from Streptococcus mutans." Chem Pharm Bull Tokyo, Vol. 38, pp. 717-720 ,1990. https://doi.org/10.1248/cpb.38.717
  18. Matsumoto M, Minami T, Sasaki H, sobue S, Hamada S, Ooshima T, "Inhibitory effects of oolong tea extract on caries-inducing properties of mutans streptococci." Caries Res, Vol. 33, pp. 441-445 ,1999. https://doi.org/10.1159/000016549
  19. Tae Hyun Youm , Heung Bin Lim, "Antimicrobial Activities of Organic Extracts from Fruit of Thuja orientalis L." Korean J. Medicinal Crop Sci, Vol. 18, No. 5, pp. 315-322 ,2010.
  20. Young Kim, Jae Woo Jung, Kang Mo Gu, Joo Young Lee, Jin Se Kim, Sang Won Yoon, Jae Chol Choi, Jong Wook Shin, Jae Yeol Kim, In Won Park, Byoung Whui Choi, "A Case of Recurrent Allergic Bronchopulmonary Aspergillosis with Weak Asthmatic Symptoms Department of Internal Medicine." Chung-Ang University College of Medicine , Vol. 120, pp. 240-241, 2015.
  21. So-Yeon Jin, "Quality Characteristics and Antioxidant Activities of Maejakgwa added Mulberry Leaf Powder." Dept. of Traditional Dietary Life Food, Graduate School, Sookmyung Women's University, pp. 140-742 ,2013.
  22. Nihal Turkmen, Ferda Sari, Y. Sedat Velioglu ."Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods." Food Chemistry, Vol. 99, No. 4, pp. 835-841, 2006. https://doi.org/10.1016/j.foodchem.2005.08.034

피인용 문헌

  1. vol.42, pp.4, 2018, https://doi.org/10.11149/jkaoh.2018.42.4.210