Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
권호 표지
DOI QR코드

DOI QR Code

Pro-inflammatory Cytokine Production Inhibitory Effects of Frankincence in Murine Macrophage

마우스의 대식세포에서 프랑킨센스의 염증성 사이토카인 분비 억제작용

  • Received : 2016.11.09
  • Accepted : 2017.01.20
  • Published : 2017.01.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study aims to demonstrate the inhibitory effect of proinflammatory cytokines by using Frankinsense. The present data was designed to determine the production of the frankincence on pro-inflammatory factors such as $TNF-{\alpha}$ and $IL-1{\beta}$ in lipopolysaccharide(LPS) stimulated RAW264.7 macrophages cell. The cell toxicity was identified by CellTiter 96 AQueous One solution cell proliferation assay. To evaluate of anti-inflammatory effect of frankincence, pro-inflammatory cytokines were measured by ELISA kit. As a result, the frankincence reduced NO and $TNF-{\alpha}$ production without cytotoxicity. As a result, Francincense was not cytotoxic at 10 ug / ml-1000 ug / ml and significantly inhibited the proinflammatory cytokines $TNF-{\alpha}$ and $IL-1{\beta}$. The secretion inhibition effect of proinflammatory cytokine is believed to be applicable to various physiological activity data and functional materials to demonstrate the anti - inflammatory properties of frankincense.

본 연구는 프랑킨센스를 이용하여 전염증성 사이토카인의 분비 억제 효과를 입증하고자한다. 이에 LPS를 처리한 마우스의 대식세포를 이용하여 $TNF-{\alpha}$, $IL-1{\beta}$와 같은 전염증성 사이토카인의 분비 변화를 살펴보기 위해 다자인하였다. 세포에 대한 독성 실험은 MTS인 CellTiter 96 AQueous One solution cell proliferation 시약을 이용하여 실시하였으며 전염증성 사이토카인의 분비는 ELISA kit을 사용하여 측정하였다. 그 결과 프랑킨센스는 10ug/ml-1000ug/ml에서 세포독성이 없었으며 전염증성 사이토카인인 $TNF-{\alpha}$, $IL-1{\beta}$생성을 유의성있게 억제시켰다. 전염증성 사이토카인의 분비 억제효과는 프랑킨센스의 항 염성을 입증하기 위한 기초적인 생리 활성 자료와 기능성 소재로 다양한 융합적인 활용이 가능하다고 사료된다.

Keywords

References

  1. Seo, S.J., Choi, H.G., Chung, H.J., Hong, C.K. "Time course of expression of mRNA of inducible nitric oxide synthase and generation of nitric oxide by ultraviolet B in keratinocyte cell lines". Br J Dermatol No. 147, pp. 655-662. 2002. https://doi.org/10.1046/j.1365-2133.2002.04849.x
  2. Shew, R.L., Papka, R.E., McNeill, D.L., Yee, J.A. "NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction". Peptides, No. 14: pp.637-641, 1993. https://doi.org/10.1016/0196-9781(93)90157-C
  3. Weller R. "Nitric oxide - a newly discovered chemical transmitter in human skin". Br J. Dermatol. No. 137: pp. 665-672, 1997. https://doi.org/10.1111/j.1365-2133.1997.tb01099.x
  4. Kwqamata H., Ochiai H., Mantani N., terasawa K. "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells, a murine macrophage cell line". Am J Chin Med No. 28: pp.217-226, 2000. https://doi.org/10.1142/S0192415X0000026X
  5. Masaki, M., Matsushita, M., Wakitani, K. "Inhibitory effect of JTE-522, a novel prostaglandin H synthase-2 inhibitor, on adjuvant-induced arthritis and bone changes in rats". Inflamm. Res No. 47:pp.187-192, 1998. https://doi.org/10.1007/s000110050316
  6. Hamm, S., Bleton, J., Connan, J. Tchapla, A. "A chemical investigation by headspace SPME AND GC-MS of volatile and semi-volatile terpens in various olibanum samples". phytochemistry . No. 66 pp. 1499-1514, 2005. https://doi.org/10.1016/j.phytochem.2005.04.025
  7. Singh, G.B., Singh, S.. Bani, S., "Anti-inflammatory actions of boswellic acids". phytomedicine No. 3 pp. 81-85, 1996. https://doi.org/10.1016/S0944-7113(96)80017-1
  8. Sharma, M. L., Kaul, A., Singh, S., Singh, G. B., "Immunomodulatory activity of boswellic acids from Boswellia serrata." phytother. Res. No. 10 pp.107-112, 1996. https://doi.org/10.1002/(SICI)1099-1573(199603)10:2<107::AID-PTR780>3.0.CO;2-3
  9. Gleich, G. J., "Mechanisms of eosinophil associated inflammation". J.Allergy Clin. Immunol. No. 105 pp. 651-663, 2000. https://doi.org/10.1067/mai.2000.105712
  10. Desai, A., Vyas T., Amiji M. "Cyroroxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations". J .Pharm Sci No. 97: pp. 2745-2756, 2008. https://doi.org/10.1002/jps.21182
  11. Rocca, B., FitzGerald, G. A. "Cyclooxygenases and Prostaglandins Shaping up the Immune Response". Int. Immunopharmacol No. 2:pp. 603-630, 2002. https://doi.org/10.1016/S1567-5769(01)00204-1
  12. MacSween, R., Whaley, K. Muir's Textbook of Pathology, 13th ed. London: Edward Arnold. 1992.
  13. Craing, C., Stitzel, R. "Introduction to CNS Pharmacology". Modern Pharmacology, 4th ed. Boston: Little, Brown & Co., pp.329, 1994.
  14. Park, J. S., Kim, M. H., "Anti-Inflammatory Effects of Rice Bran Ethanol Extract in Murine Macrophage RAW 264.7 Cells", Yakhak Hoeji No. 6 pp.456-461, 2011.
  15. Delanty, N., Dichter, M. A. "Oxidative Injury in the Nervous Syste,". Acta Neurol. Scand 1998;98: 145-153. https://doi.org/10.1111/j.1600-0404.1998.tb07285.x
  16. Kiyoshi, T., Tsuneyasu, K., Shizuo, A. "Toll-like receptors", Annu. Tev. Immunol No. 21: pp. 335-376. 2003. https://doi.org/10.1146/annurev.immunol.21.120601.141126
  17. Jeong , S. H., Park, J. S., "Anti-Inflammatory Effects of Myrrrh Oil in Murine Macrophage RAW 264.7 Cells."J. Kor. Soc. Cosm. Vol. 16, No. 4, pp. 1113-1118, 2010
  18. Lee, K. Y., Kim, J. H., Son, J. R., Lee, J. S. "Detection and Extraction Condition of Physiological Functional Compounds from Bran of Heugjinju Rice (Oryza Sativa L)", Korean J. Postharvest Sci.. Technol pp. 296-301. 2001.
  19. Jae-Hyeok Lee, Jeong-Suk Park, "Antioxidant Activities of Rice Bran Extracts for Wellness Convergence ", Journal of digital Convergence , Vol. 13, No. 2, pp. 401-406, 2015. https://doi.org/10.14400/JDC.2015.13.2.401
  20. Chen, F., Castranova, V., Shi, X. "New Insights into the Role of Nuclear Factor-KappaB in Cell Growth Regulation", Am. J. Pathol pp. 389-397. 2001.