한국융합학회논문지 (Journal of the Korea Convergence Society)

  • 제8권1호
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  • Pages.107-114
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  • 2017
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  • 2233-4890(pISSN)
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  • 2713-6353(eISSN)
한국융합학회 (Korea Convergence Society)
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천년초의 융합발효를 통한 특성 연구

The Characterization of L. plantarum-fermented Opuntia humifusa

  • 투고 : 2016.11.15
  • 심사 : 2017.01.20
  • 발행 : 2017.01.28
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초록

본 연구는 생리활성이 우수한 천년초의 활용도를 높이기 위해 L. plantarum를 이용한 발효과정을 통해 얻은 추출물을 천년초의 열수추출물과 비교하여 특성을 확인하였다. 점성분석, 총폴리페놀, 아미노산 분석을 수행하였으며, 항박테리아 활성 및 사이토카인 분석, zymography 등을 통해 발효추출물의 특성을 연구하였다. 천년초 이용도를 절감시키는 점성도가 거의 절반 감소되고, 비타민 C의 양은 약간 감소하였으나 발효과정 동안 거의 유지되는 것으로 보이며, 총폴리페놀, 아미노산 총량 및 생리활성 주요 무기질 성분은 발효를 통해 증가함을 확인하였다. 또한 세포독성이 없음을 확인하고, 염증관련 사이토카인류와 MMP-9 활성도를 확인한 결과 천년초의 열수추출물에 비해 L. plantarum-발효 천년초에서 염증 억제 및 조직 파괴 억제 효과가 있는 것으로 확인되었다. 이러한 결과들은 유산균을 이용한 발효 천년초를 기능성을 부가한 소재로 활용하는데 있어 기초가 될 것으로 기대된다.

To increase the bioavailability of Opuntia humifusa, O. humifusa was fermented using L. plantarum, and the characteristics of the fermented extract were confirmed. The characteristics of fermented extracts were investigated through viscosity, total polyphenol content, amino acid, antibacterial activity, cytokine analysis and zymography. The viscosity decreased by half and vitamin C remained almost unchanged during fermentation. Total polyphenols, most amino acids, total amino acids and major minerals were increased by fermentation. There was no cytotoxicity, and the activity of cytokines and MMP-9 in inflammation was inhibited. The inhibitory effect on inflammation and tissue destruction was found to be inhibited in L. plantarum fermentation extracts compared to hot water extracts. These results are expected to be the basis for the development of materials that enhance the functionality of L. plantarum-fermented O. humifusa extract.

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