Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Enhanced physiological activities of wine yeasts Pichia manshurica and Pichia terricola isolated from grapes pericarp

포도 과피에서 분리한 효모 Pichia manshurica와 Pichia terricola의 생리활성 증진효과

  • Park, Sang-Kook (Department of Life Science and Biotechnology, Soonchunhyang University) ;
  • Kim, Dong-Min (Department of Biotechnology, The University of Tokyo) ;
  • Oh, Kye-Heon (Department of Life Science and Biotechnology, Soonchunhyang University)
  • 박상국 (순천향대학교 자연과학대학 생명시스템학과) ;
  • 김동민 (도쿄대학교 생명공학과) ;
  • 오계헌 (순천향대학교 자연과학대학 생명시스템학과)
  • Received : 2017.08.16
  • Accepted : 2017.09.22
  • Published : 2017.12.31
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Abstract

The purpose of this study was to investigate the enhanced physiological activities of two Pichia strains, yeasts isolated from grapes pericarp. Based on phylogenetic analysis using 18S rRNA sequencing, two isolates were identified as Pichia manshurica GU-3 and Pichia terricola GU-4, respectively. The scanning electron microscopic analysis showed that the two isolates grown on YPD medium were of typical elliptical shape with buds and bud scars on cell surface. Physiological activities of the single and mixed Pichia cultures were monitored and compared. In mixed cultures after 72 h of incubation, the maximum activities of tyrosinase inhibition, ACE inhibition, and antioxidant were 81.7%, 45.9%, and 42.7%, respectively. Superoxide dismutase-like activity was approximately 30% in the mixed cultures. These studies demonstrate that Pichia species cultured in the form of mixture can enhance the physiological activities and has potential for the development of new bioactive products.

본 연구는 포도의 과피에서 분리한 효모 Pichia의 생리활성 증진효과를 연구하기 위한 것이다. 두 균주는 18S rRNA 염기서열분석에 근거하여 Pichia manshurica GU-3와 Pichia terricola GU-4로 각각 동정되었다. 주사전자현미경 분석을 통해 YPD 배지에서 배양한 두 분리세균은 세포표면에 출아와 출아흔적을 가지고 있었으며 전형적인 타원형을 보여주었다. 단일 및 혼합 Pichia 배양의 생리활성을 비교 관찰한 결과, 72시간 배양한 후, 혼합배양에서 tyrosinase억제, ACE 억제, 항산화 최대활성은 각각 81.7%, 45.9%, 42.7%로 나타났다. Superoxide dismutase-유사활성은 혼합배양에서 약 30%로 측정되었다. 이 연구를 통해서 혼합배양된 Pichia 종들은 생리활성을 증진시켰으며, 생리활성을 가지는 산물 개발의 가능성을 가지는 것으로 입증되었다.

Keywords

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