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구강안면통증모델에서 산자나무 추출물의 진통효과

Analgesic Effect of Hippophae rhamnoides Extract in Orofacial Pain in Rats

  • 김윤경 (동의대학교 일반대학원 보건의과학과) ;
  • 최자형 (동의대학교 일반대학원 보건의과학과) ;
  • 김희진 (동의대학교 일반대학원 보건의과학과) ;
  • 윤현서 (동의대학교 일반대학원 보건의과학과) ;
  • 현경예 (동의대학교 일반대학원 임상병리학과) ;
  • 이민경 (동의대학교 일반대학원 보건의과학과)
  • Kim, Yun-Kyung (Department of Biomedical Health Science, Dong-Eui University) ;
  • Choi, Ja-Hyeong (Department of Biomedical Health Science, Dong-Eui University) ;
  • Kim, Hee-Jin (Department of Biomedical Health Science, Dong-Eui University) ;
  • Yoon, Hyun-Seo (Department of Biomedical Health Science, Dong-Eui University) ;
  • Hyun, Kyung-Yae (Department of Clinical Laboratory Science, Dong-Eui University) ;
  • Lee, Min-Kyung (Department of Biomedical Health Science, Dong-Eui University)
  • 투고 : 2017.09.04
  • 심사 : 2017.10.12
  • 발행 : 2017.12.31

초록

본 연구는 실험동물 대상으로 하는 두 가지 구강안면통증모델을 이용하여 산자나무 추출물 H. rhamnoides (sea buckthorn)의 통증조절효과를 확인하고자 하였다. 5% formalin을 실험동물 안면부 피하($50{\mu}l$) 또는 측두하악관절($30{\mu}l$) 내로 주입하여 안면부를 긁거나 문지르는 통증행위반응을 유도하였다. Formalin의 주입으로 인한 안면부통증행위반응은 15분 이후부터 증가하여 25, 30분에 가장 높게 나타났으며 40분까지 지속되다가 45분에 감소되었다. 안면부 통증 유발 30분 전 150 mg, 300 mg/kg (1 ml)의 농도로 산자나무 추출물을 희석하여 경구투여하였다. DW를 경구투여한 대조군에서는 formalin에 의한 통증행위반응의 변화에 영향을 미치지 않았으나 산자나무 추출물(150, 300 mg/kg)의 경구투여는 각 $305.3{\pm}20.7$회, $137.3{\pm}21.8$회로 포르말린으로 유도된 안면부통증행위반응을 감소시켰다. 시간에 따른 변화의 결과로, 산자나무 추출물의 300 mg/kg 경구투여는 25~40분에서 formalin에 의해 증가된 안면부통증행위반응을 현저하게 감소시켰다. 측두하악관절에 주입한 포르말린은 $306.8{\pm}31.4$회의 통증행위반응을 나타내어 턱관절 통증을 유도하였고, 산자나무 추출물(150, 300 mg/kg)의 경구투여는 턱관절통증행위반응을 각 $184.7{\pm}20.0$회, $125.7{\pm}9.8$회로 감소시켰다. 시간의 경과에 따라 결과에서 산자나무 추출물의 경구투여는 포르말린 주입 30분에서 유의한 턱관절 통증경감효과를 나타냈다. 따라서, 본 연구의 결과는 산자나무 추출물의 구강안면 통증조절에 대한 예방적 또는 치료적 제제로의 활용가능성에 대한 기초자료로 활용가능할 것으로 생각된다.

Hippophae rhamnoides L. (sea buckthorn) is a shrub wood that belongs to the bamboo tree family, and is rich in vitamin C, D, and E; it is referred to as a vitamin tree. It is mainly grown in the high mountains of Europe and Central Asia, and has been widely used in China and Russia as natural medicine. Recent studies have shown that it is effective in the treatment of cancer, liver diseases, cardiovascular diseases, and gastrointestinal diseases. However, results of studies on its effect on the regulation of pain are insufficient. In this study, we investigated the effect of sea buckthorn on the development and control of pain in two facial areas. The experimental animals included 7- to 8-week-old Sprague-Dawley rats (240~260 g). Formalin (5%), which is known as an inflammation inducer, was injected into the vibrissa pad or temporomandibular joints to induce orofacial acute pain. Rubbing or scraping of the region injected with formalin was regarded as a pain index, and the behavioral response was observed for 45 minutes after the injection. Sea buckthorn extract diluted to 150, 300 mg/kg (in 1 ml of distilled water) was orally administered 30 minutes prior to the acute pain. The facial pain behavior was effectively reduced in the 300 mg/kg group when compared to the control group (vehicle). Likewise, in an experiment in which formalin was injected into the temporomandibular joints, effective pain alleviation was confirmed at the same drug concentration. These results suggest that sea buckthorn extract may be useful in the development of therapeutic agents for acute inflammatory pain in the orofacial area and for controlling temporomandibular joint pain.

키워드

참고문헌

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피인용 문헌

  1. Orofacial Pain Anti-Inflammatory Activity of Extracts of Boswellia Serrata in Rats vol.24, pp.3, 2018, https://doi.org/10.15616/bsl.2018.24.3.239