Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Polymorphism of inter simple sequence repeat markers in Hypsizygus marmoreus

Inter Simple Sequence Repeat(ISSR) 마커를 활용한 느티만가닥버섯(Hypsizigus marmoreus) 종내 다형성 분석

  • Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Nam, Yun-Geul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Min ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Im, Ji-Hoon (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, In-Geol (Department of Biotechnology College of Life Sciences and Biotechnology Korea University)
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 남윤걸 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오민지 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 임지훈 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 최인걸 (고려대학교 생명공학과 대학원 생명공학과&BK21플러스)
  • Received : 2017.09.04
  • Accepted : 2017.11.27
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Hypsizygus marmoreus is a mushroom with abundant flavor and medicinal properties. However, its application is limited by problems such as long cultivation period, low biological efficiency, and microbiological contamination; therefore, there is a substantial need for development of new cultivars of this species. In this study, 55 strains of H. marmoreus were subjected to inter simple sequence repeat (ISSR) analysis to identify markers for the selection of mother strains for breeding from the collected germplasm. ISSR 13 and 15 were confirmed as polymorphic markers. The three strains (KMCC03106, KMCC03107, and KMCC03108) with white cap color were found to be genetically closely related upon UPGMA analysis of both ISSR 13 and 15. Based on the PCR analysis results for ISSR 15, the collected germplasm were differentiated into three groups according to the strain collection year. Thus, ISSR 15 could be a marker for determining the phylogeny of cap color and genetic variations according to the strain collection year. These results suggest that ISSR markers can be effective tools for the selection of mother strains for breeding of H. marmoreus.

느티만가닥버섯은 맛과 기능성이 풍부한 버섯으로 많이 활용되고 있다. 하지만 긴 재배기간과 낮은 자실체 수확량, 균이 오랜시간 배양되어 오염이 쉽게 발생되는 문제점을 극복할 새로운 품종육성이 필요하다. 이에 따라 육종모본으로 활용되는 느티만가닥 55균주의 종내 유전자원의 정확한 정보를 얻고자 분자유전학적 ISSR 마커분석을 활용하였다. 사용된 마커 중에서 ISSR 13과 15 마커를 사용했을 때 다형성이 분석되었으며 특히 ISSR 15마커 분석으로 다형성이 쉽게 구분되었다. UPGMA분석법으로 계통도를 분석하였을 때, 일부 백색을 가지고 있는 KMC03106, KMC03107, KMC03108 3 균주가 두 마커 모두에서 가까운 유연관계를 가졌으며, ISSR 15는 수집년도에 따라 3개의 그룹으로 구분되는 것을 확인할 수 있었다. 이 결과로 ISSR마커의 다형석 분석은 느티만가닥버섯의 몇몇 균주에서는 갓색의 유연관계 확인과 수집 시기에 따른 유전변이 구분이 가능하며 자원의 유전적 다양성 확인할 수 있어, 느티만가닥버섯의 품종육성을 위한 효율적인 모본 선발이 가능 할 것으로 사료된다.

Keywords

References

  1. Ali M. L., Rajewski J. F., Baenziger P. S., Gill K. S., Eskridge K. M., Dweikat I. 2008. Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplsm by SSR markers. Mol Breed. 21:497-509. https://doi.org/10.1007/s11032-007-9149-z
  2. Alam N., Shim M. J., Lee M. W., Shin P. G., Yoo Y. B., Lee T. S. 2009a. Phylogenetic relationship in different commercial strains of Pleurotus nebrodensis based on ITS sequence and RAPD. Mycobiology. 37:183-188. https://doi.org/10.4489/MYCO.2009.37.3.183
  3. Alam N., Shim M. J., Lee M. W., Shin P. G., Yoo Y. B., Lee T. S. 2009b. Vegetative growth and phylogenetic relationship of commercially cultivated strains of Pleurotus eryngii based on ITS sequence and RAPD. Mycobiology. 37:258-266. https://doi.org/10.4489/MYCO.2009.37.4.258
  4. Al-Rawashdeh I. M. 2011. Genetic variability in a medicinal plant Artemisia judaica using random amplified polymorphic DNA(RAPD) markers . Int J Agric Biol. 13:278-282.
  5. Behera T. K., Gaikward A. B., Singh A. K., Staub J. E. 2008. Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). J Sci Food Agric. 88:733-737. https://doi.org/10.1002/jsfa.3145
  6. Bohn M., Utz H. F., Melchinger A. E. 1999. Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs, and SSRs and their use for predicting progeny variance. Crop Sci. 39:228-237. https://doi.org/10.2135/cropsci1999.0011183X003900010035x
  7. Camacho J. F., Liston A. 2001. Population structure and genetic diversity of Botrychinm pumicola(Ophioglossaceae) based on inter simple sequence repeats (ISSR). Amer J Bot. 88:1065-1070. https://doi.org/10.2307/2657089
  8. Castle A. J., Horgen P. A., Anderson J. B. 1987. Restriction fragment length polymorphism in the mushrooms Agaricus brunnescens and Agaricus bitorquis. Appl Environ Microbiol. 53:816-822.
  9. Gonzalez P., Labarere J. 2000. Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6, and V9 domains. Mycobiology 146:209-221.
  10. Hu K. L., Chu X. P. 2008. Edible fungi china. 95:891-904.
  11. Lam S. K. and Ng T. B. 2001. Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochem Biophys Res Commun. 285: 1071-1075. https://doi.org/10.1006/bbrc.2001.5279
  12. Lim Y. J., Lee C. Y., Park J. E., Kim S. W., Lee H. S., Ro H. S. 2010. Molecular genetic classification of Hypsizigus marmoreus and development of strain-specific DNA marks. Kor J Mycol. 38:34-39. https://doi.org/10.4489/KJM.2010.38.1.034
  13. Lin K. H., Lai Y. C., Li H. C., Lo S. F., Chen L. F. O., Lo H. F. 2009. Genetic variation and its relationship to root weight in the sweet potato as revealed by RAPD analysis. Sci Hortic. 120: 2-7. https://doi.org/10.1016/j.scienta.2008.09.008
  14. Park H. G., Ko H. G., Kim S. H., Park W. O. 2004. Molecular identification of Asian isolates of medicinal mushroom Hericium erinaceum by phylogenetic analysis of nuclear ITS rDNA. J Microbiol Biotechnol. 14:816-821.
  15. Patzak J. 2001. Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus Iupulus L.). Euphytica. 121:9-18. https://doi.org/10.1023/A:1012099123877
  16. Qiu C., Yan W., Peng L., Deng W., Song B., Li T. 2013. Evaluation of growth characteristics and genetic diversity of commercial and stored lines of Hypsizigus marmoreus. Int J Agaric Biol. 15:479-485.
  17. Sabou L., Brhada F., Alami I. T., Maltouf A. F. 2010. Genetic diversity of Moroccan Lupinus germplasm investigated using ISSR and AFLP markers. Int J Agric Biol. 12:26-32.
  18. Sun S., Li Z., Ruan L., Zhang L., Hu K. 2014. A novel breeding strategy for new strains of Hypsizigus marmoreus and Grifola frondasa based on ligninolytic enzymes. World J Microbiol Biotechnol. 30:2005-2013. https://doi.org/10.1007/s11274-014-1624-1
  19. Wang L., Hu W., Feng Z., Pan Y. 2009. Developemnt of AFLP markers and phylogenetic analaysis in Hypsizigus marmoreus. J Gen Appl Microbial. 55:9-17. https://doi.org/10.2323/jgam.55.9
  20. Xu J., Kerrigan R. W., Callac P., Horgen P. A., Anderson J. B. 1997. Genetic structure of natural populations of Agaricus bisporus, the commercial button mushroom. J Hered. 88:482-488. https://doi.org/10.1093/oxfordjournals.jhered.a023141
  21. Zhang Y. F., Molina F. I. 1995. Strain typing of Letinula edodes by random amplified polymorphic DNA assay. FEMS Microbiol Lett. 131:171-20.
  22. Zietkiewicz E., Kafalskl A., Labuda D. 1994. Genome fingerprinting by simple sequence repeats (SSR) - anchored polymerase chain reaction amplification. Genomics. 20:176-183. https://doi.org/10.1006/geno.1994.1151