Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Changes of chemical compositions of cereals by Phellinus linteus mycelial cultivation

상황진흙버섯 균사배양 곡물의 성분변화

  • 진성우 ((재)장흥군버섯산업연구원) ;
  • 임승빈 ((재)장흥군버섯산업연구원) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 윤경원 (순천대학교 한약자원개발학과) ;
  • 정상욱 ((재)장흥군버섯산업연구원) ;
  • 고영우 ((재)장흥군버섯산업연구원) ;
  • 제해신 ((재)장흥군버섯산업연구원) ;
  • 조인경 (남부대학교 식품영양학과) ;
  • 장지연 (한국생산기술연구원) ;
  • 서경순 ((재)장흥군버섯산업연구원)
  • Received : 2017.09.04
  • Accepted : 2017.11.10
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the physicochemical characteristics of cultivated cereals by Phellinus linteus mycelium. Also, we investigated the content of free sugar, organic acid, free amino acid, mineral and fatty acid of cultivated cereals by P. linteus mycelium. The major free sugars were determined the glucose, maltose, and fucose by HPLC. The content of total free sugars was the highest in cultivated red rice by P. linteus mycelium at $36^{\circ}C$. The content of fucose was the highest in cultivated red rice by P. linteus mycelium at all cultivation temperatures. The organic acids were identified oxalic acid, malic acid, citric acid, and succinic acid, as in the case of free sugar, higher contents of organic acids were observed in the cultivated cereals by P. linteus mycelium than control. There was no significant difference in the mineral content between the control and cultivated cereals by P. linteus mycelium. Sixteen kind of free amino acid were detected in cultivated cereals by P. linteus mycelium. And the content of total free amino acids were higher in the cultivated cereals by P. linteus mycelium than the control. And essential amino acids showed significantly differences between control and cultivated cereals by P. linteus mycelium. Fatty acids were detected with seven kinds of fatty acids, and the major fatty acid was determined the linoleic acid by GC-MS. The content of linoleic acid was higher in the cultivated cereals by P. linteus mycelium than the control.

본 연구는 곡물(백미, 적미, 흑미)에 상황진흙버섯 균사를 배양하여 곡물과 상황진흙버섯의 유용성분 시너지 효과를 탐색하고자 수행하였으며, 배양조건에 따른 상황진흙버섯 균사 배양곡물의 유리당, 유기산, 유리아미노산, 무기성분, 지방산을 분석하였다. 유리당의 함량을 분석한 결과 fucose, glucose, maltose 총 3종의 유리당이 검출되었으며, 대조구에서는 glucose에서만 검출되었다. 상황진흙버섯을 배양한 곡물들에서 대조구에 비해 높은 유리당 함량이 검출되었다. 상황진흙버섯을 배양한 곡물들의 유기산 함량을 분석한 결과, 4종의 유기산이 검출되었으며 유리당과 마찬가지로 상황진흙버섯을 접종한 시료구에서 대조구에 비해 높은 함량의 유기산이 확인되었다. 상황진흙버섯을 배양한 곡물들과 대조구간의 무기성분 함량 차이는 유의적이지 않았다. 16종의 아미노산이 검출되었으며, 상황진흙버섯을 배양한 곡물들에서 유리아미노산의 함량이 다량 증가함을 확인하였다. 지방산의 결과는 총 7종의 지방산이 검출되었으며 각 대조구와 상황진흙버섯을 배양한 곡물들간에 유의적인 차이는 나타나지 않았지만, 배양 원료로 사용한 백미와 유색미와의 조성에서 많은 차이가 있었다.

Keywords

References

  1. Lee TS, 1990. The full list of recoded mushroom in Korea. Kor J Mycol. 18:233-259.
  2. Han SB, Lee CW, Kang JS, Yoon YD, Lee KH, Lee K, Park SK, Kim HM. 2006. Acidic polysaccharide from Phellinus linteus inhibits melanoma cell metastasis by blocking cekk adhesion and invasion. Int Immunopharmacol, 6:697-702 https://doi.org/10.1016/j.intimp.2005.10.003
  3. Fujii T, Maeda H, Suzuki F, Ishida N. 1978. Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes. J Antiniot. 31:1079-1090. https://doi.org/10.7164/antibiotics.31.1079
  4. Suzuki S and Ohshima S. 1976. Influence of shiitake (Lentinus edodes) on human serum cholesterol. Mushroom Sci. 9:463-647.
  5. Hikino H, Konno C, Mirin Y, Hayashi T. 1985. Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies1. Plant Med. 51:339-340. https://doi.org/10.1055/s-2007-969507
  6. Cho EJ, Hwang HJ, Kim SW, Oh JY, Baek YM, Choi JW, Bae SH, Yun JW, 2007. Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice. Appl Microbiol Biotechnol. 75:1257-1265. https://doi.org/10.1007/s00253-007-0972-2
  7. Choi JH, Ha TH, Rho YD, 1996. Studied on the main factors affecting the mycelial growth of Phellinus linteus. Kor J Mycol. 24:214-222.
  8. Kim HM, Lee DH. 2010. Characterization of anti-inflammation effect of aqueous extracts from Phellinus baumii. Kor J Mycol. 38:179-183. https://doi.org/10.4489/KJM.2010.38.2.179
  9. Kim DH, Han SB, Oh GT, Kim YH, Hong DH, Hong ND, Yoo ID. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int J Immunophamacol. 18:295-303. https://doi.org/10.1016/0192-0561(96)00028-8
  10. Lee KH, Kwon HJ, Chun SS, Kim JH, Cho YJ, Cha WS. 2006. Biological activities if extracts from Phellinus linteus. J. Korean Soc Appl Biol Chem. 49:298-303.
  11. Choi HD, Seog HM, Park YK, Park YD, Kim JA, 2007 Hypoglycemic effects of Basidiomycetes mycelia and cereals fermented with Basidiomycetes. J Korean Soc Food Sci Nutr. 36:1257-1262. https://doi.org/10.3746/jkfn.2007.36.10.1257
  12. Lee H, Kim YS, Kim DY, Kim SY, Lee WK, Lee SM, Park JD, Shon MY. 2015. A study on manufacturing of red ginseng Makgeolli using the red ginseng starch and changes of physicochemical components of red ginseng Makgeolli during storage periods. Korean J Food Preserv. 22:369-376 https://doi.org/10.11002/kjfp.2015.22.3.369
  13. Nicolsi RJ, Rogers EJ, Ausman LM, Orthefer FT. 1993. Rice bran oil and its health benefits. In: Rice Science and Technology. Boca Raton, FL, USA pp. 421-437.
  14. Capro PA, Reaven G, Olefsky J. 1977. Postgrandial plasmaglucose and insulin responses to different complex carbohydrate. Diabetes. 26:1178-1183. https://doi.org/10.2337/diab.26.12.1178
  15. Rhee CO. Song, Lee YS. 2000. Volatile flavor components in cooking black rice. Korea J Food Sci Technol. 32:1015-1021.
  16. Koh HJ, Won YJ, Cha GW, Heu MH. 1996. Amino acids, vitamins and mineral contents of some colored rices. Annual Report of Agriculture and Life Science, p.88-89.
  17. Lee JT, Jeong YS, An BJ. 2002. Physiological activity of Salicornia herbacea and its application for cosmetic materials. Kor J Herbology. 17:51-60.