Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지
DOI QR코드

DOI QR Code

In vitro Antimicrobial Effects of Silver Nanoparticles on Microorganisms Isolated from Dog with Otitis External

  • Bae, Seul-gi (College of Veterinary Medicine, Kyungpook University) ;
  • Yun, Sung-ho (College of Veterinary Medicine, Kyungpook University) ;
  • Oh, Tae-ho (College of Veterinary Medicine, Kyungpook University)
  • Received : 2017.11.22
  • Accepted : 2017.12.08
  • Published : 2017.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Silver nanoparticles have marked antimicrobial effects on several pathogens and have been used to control bacterial growth in humans. In the present study, we evaluated the antimicrobial efficacy of silver nanoparticles against the common causative pathogens of canine otitis external through counting of colony forming units. Silver nanoparticles showed significant dose-dependent antimicrobial effects on pathogens. In addition, we conducted antimicrobial susceptibility tests and compared the antimicrobial efficacy of silver nanoparticles. Microorganisms with a high resistance to antibiotics were also resistant silver nanoparticle with low concentration ($5{\mu}g/mL$). However, in high concentration ($15{\mu}g/mL$), almost 100% reduction in the number of CFUs of these pathogens was observed.

Keywords

References

  1. Ahmad Z, Pandey R, Sharma SKhuller G. Alginate nanoparticles as antituberculosis drug carriers: formulation development, pharmacokinetics and therapeutic potential. Indian J Chest Dis Allied Sci 2006; 48: 171-176.
  2. Alivisatos AP. Perspectives on the physical chemistry of semiconductor nanocrystals. J Phys Chem 1996; 100: 13226-13239. https://doi.org/10.1021/jp9535506
  3. Bosetti M, Masse A, Tobin ECannas M. Silver coated materials for external fixation devices: in vitro biocompatibility and genotoxicity. Biomaterials 2002; 23: 887-892. https://doi.org/10.1016/S0142-9612(01)00198-3
  4. Gu H, Ho P, Tong E, Wang L, Xu B. Presenting vancomycin on nanoparticles to enhance antimicrobial activities. Nano Lett 2003; 3: 1261-1263. https://doi.org/10.1021/nl034396z
  5. Jain J, Arora S, Rajwade JM, Omray P, Khandelwal S, Paknikar KM. Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use. Mol Pham 2009; 6: 1388-1401. https://doi.org/10.1021/mp900056g
  6. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park Y, Hwang CY. Antimicrobial effects of silver nanoparticles. Nanomedicine 2007; 3: 95-101. https://doi.org/10.1016/j.nano.2006.12.001
  7. Kwon GH, Kim JE, Seo KW, Kim YB, Jeon HY, Lee KW, Oh TH, Yi SJ, Kim SJ, Kim KS, Song JC, Kim TW, Lee YJ. Antimicrobial resistance of methicillin-resistant staphylococci isolates from dog ears in korea. J Vet Clin 2017; 34: 335-340. https://doi.org/10.17555/jvc.2017.10.34.5.335
  8. Lara HH, Ayala-Nunez NV, Turrent LCI, Padilla CR. Bactericidal effect of silver nanoparticles against multidrugresistant bacteria. World J Microbiol Biotechnol 2010; 26: 615-621. https://doi.org/10.1007/s11274-009-0211-3
  9. Li Y, Leung P, Yao L, Song Q, Newton E. Antimicrobial effect of surgical masks coated with nanoparticles. J Hosp Infect 2006; 62: 58-63. https://doi.org/10.1016/j.jhin.2005.04.015
  10. Park SY, Bae SG, Kim JT, Oh TH. Identification and antimicrobial suceptibility of bacteria isolated from dogs with chronic otitis externa. J Vet Clin 2017; 34: 23-26. https://doi.org/10.17555/jvc.2017.02.34.1.23
  11. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 2009; 27: 76-83. https://doi.org/10.1016/j.biotechadv.2008.09.002
  12. Rai M, Deshmukh S, Ingle AGade A. Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbiol 2012; 112: 841-852. https://doi.org/10.1111/j.1365-2672.2012.05253.x
  13. Bae SG, Choi SW, Kim BM, Lee YG, Oh TH. Efficacy of enrofloxacin and silver sulfadiaznine topical otic suspension for the treatment of canine otitis externa. J Vet Clin 2013; 30: 172-177.
  14. Silver S, Phung L TSilver G. Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds. J Ind Microbiol Biotechnol 2006; 33: 627-634. https://doi.org/10.1007/s10295-006-0139-7
  15. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 2004; 275: 177-182. https://doi.org/10.1016/j.jcis.2004.02.012
  16. Tian J, Wong KK, Ho CM, Lok CN, Yu WY, Che CM, Chiu J F, Tam PK. Topical delivery of silver nanoparticles promotes wound healing. Chem Med Chem 2007; 2: 129-136. https://doi.org/10.1002/cmdc.200600171
  17. Watts JL. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard, 3rd ed. Wayne: Pensylvania. 2008.
  18. Yoshida K, Tanagawa MAtsuta M. Characterization and inhibitory effect of antibacterial dental resin composites incorporating silver-supported materials. J Biomed Mater Res 1999; 47: 516-522. https://doi.org/10.1002/(SICI)1097-4636(19991215)47:4<516::AID-JBM7>3.0.CO;2-E

Cited by

  1. Antibiofilm activity of silver nanoparticles against biofilm forming Staphylococcus pseudintermedius isolated from dogs with otitis externa vol.7, pp.5, 2021, https://doi.org/10.1002/vms3.554