한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제34권6호
  • /
  • Pages.410-413
  • /
  • 2017
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지
DOI QR코드

DOI QR Code

Biodegradable Hydroxyapatite/Chitosan Composites on the Bone Defect of Canine Model

  • Kim, Jooho (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Dongbin (College of Veterinary Medicine, Western University of Health Science) ;
  • Heo, Suyoung (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Namsoo (College of Veterinary Medicine, Chonbuk National University)
  • 투고 : 2017.11.24
  • 심사 : 2017.12.13
  • 발행 : 2017.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Composites of hydroxyapatite (HAp) and chitosan (CS) have been successfully used in bone healing in humans and animals. However, the characteristics of HAp and CS are different. Therefore, the effects of HAp/CS composites on canine bone formation could differ according to their ratio. This study investigated the therapeutic effects of different contents ratios (100, 80:20, 60:40 wt%) on bone defects in a canine model. Thirty intrabony cylindrical defects were created in the humeruses and femurs of 5 beagle dogs, and then the defects were implanted with different composites. The evaluations were performed using radiographs obtained at 10 weeks post-surgery and by histological findings. In radiographic evaluation including the grades of bone filling, periosteal and endosteal reactions, pure hydroxyapatite composite had a significant effect on bone filling, and chitosan containing the composites showed vigorous responses at the periosteum and endosteum. In histological findings, the defect implanted with pure hydroxyapatite had healed completely into mature bony tissue with an obvious osteon structure, and the defect implanted with chitosan containing the composites had the amount of fibrous connective tissue increased significantly within the cortical bone tissue. The results indicate that hydroxyapatite/chitosan composites are therapeutically useful, promoting effective bone healing in defects when the ratio of hydroxyapatite is high and enhanced fibrous connective tissue formation at the periosteum as more chitosan is added.

키워드

참고문헌

  1. Belmonte MM, De Benedittis A, Muzzarelli R, Mengucci P, Biagini G, Gandolfi M, Zucchini C, Krajewski A, Ravaglioli A, Roncari E. Bioactivity modulation of bioactive materials in view of their application in osteoporotic patients. J Mater Sci Mater Med 1998; 9: 485-492. https://doi.org/10.1023/A:1008827619290
  2. Chevrier A, Hoemann C, Sun J, Buschmann M. Chitosanglycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects. Osteoarthritis Cartilage 2007; 15: 316-327. https://doi.org/10.1016/j.joca.2006.08.007
  3. Hasegawa S, Ishii S, Tamura J, Furukawa T, Neo M, Matsusue Y, Shikinami Y, Okuno M, Nakamura T. A 5-7 year in vivo study of high-strength hydroxyapatite/poly (Llactide) composite rods for the internal fixation of bone fractures. Biomaterials 2006; 27: 1327-1332. https://doi.org/10.1016/j.biomaterials.2005.09.003
  4. Ito M, Hidaka Y, Nakajima M, Yagasaki H, Kafrawy A. Effect of hydroxyapatite content on physical properties and connective tissue reactions to a chitosan-hydroxyapatite composite membrane. J Biomed Mater Res 1999; 45: 204-208. https://doi.org/10.1002/(SICI)1097-4636(19990605)45:3<204::AID-JBM7>3.0.CO;2-4
  5. Lu W, Zhao F, Luk K, Yin Y, Cheung K, Cheng G, Yao K, Leong J. Controllable porosity hydroxyapatite ceramics as spine cage: fabrication and properties evaluation. J Mater Sci Mater Med 2003; 14: 1039-1046. https://doi.org/10.1023/B:JMSM.0000004000.56814.9e
  6. enel S, McClure SJ. Potential applications of chitosan in veterinary medicine. Adv Drug Deliv Rev 2004; 56: 1467-1480. https://doi.org/10.1016/j.addr.2004.02.007
  7. Sunny M, Ramesh P, Varma H. Microstructured microspheres of hydroxyapatite bioceramic. J Mater Sci Mater Med 2002; 13: 623-632. https://doi.org/10.1023/A:1015709705074
  8. Uchida A, Araki N, Shinto Y, Yoshikawa H, Kurisaki E, Ono K. The use of calcium hydroxyapatite ceramic in bone tumour surgery. J Bone Joint Surg Br 1990; 72: 298-302.
  9. Ueno H, Yamada H, Tanaka I, Kaba N, Matsuura M, Okumura M, Kadosawa T, Fujinaga T. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials 1999; 20: 1407-1414. https://doi.org/10.1016/S0142-9612(99)00046-0
  10. Usami Y, Okamoto Y, Minami S, Matsuhashi A, Kumazawa NH, Tanioka S, Shigemasa Y. Migration of canine neutrophils to chitin and chitosan. J Vet Med Sci 1994; 56: 1215-1216. https://doi.org/10.1292/jvms.56.1215
  11. Usami Y, Okamoto Y, Takayama T, Shigemasa Y, Minami S. Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2. J Biomed Mater Res 1998; 42: 517-522. https://doi.org/10.1002/(SICI)1097-4636(19981215)42:4<517::AID-JBM6>3.0.CO;2-U
  12. Yin Y, Ye F, Cui J, Zhang F, Li X, Yao K. Preparation and characterization of macroporous chitosan-gelatin/${\beta}$-tricalcium phosphate composite scaffolds for bone tissue engineering. J Biomed Mater Res 2003; 67: 844-855.
  13. Zhang Y, Xu HH. Effects of synergistic reinforcement and absorbable fiber strength on hydroxyapatite bone cement. J Biomed Mater Res 2005; 75: 832-840.
  14. Zhang Y, Xu HH, Takagi S, Chow LC. In-situ hardening hydroxyapatite-based scaffold for bone repair. J Mater Sci Mater Med 2006; 17: 437-445. https://doi.org/10.1007/s10856-006-8471-z
  15. Zhao F, Yin Y, Lu WW, Leong JC, Zhang W, Zhang J, Zhang M, Yao K. Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds. Biomaterials 2002; 23: 3227-3234. https://doi.org/10.1016/S0142-9612(02)00077-7