Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Toxicity Assessment of Phenanthrene using the Survival and Population Growth Rate of the Marine Rotifer, Brachionus plicatilis

해산로티퍼(Brachionus plicatilis)의 생존 및 개체군 성장률을 이용한 Phenanthrene의 독성평가

  • Hwang, Un-Ki (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Choi, Hoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Jang, Soo-Jung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Heo, Seung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS) ;
  • Lee, Ju-Wook (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, NIFS)
  • 황운기 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 최훈 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 장수정 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 허승 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 이주욱 (국립수산과학원 서해수산연구소 해양생태위해평가센터)
  • Received : 2017.11.13
  • Accepted : 2017.12.04
  • Published : 2017.12.31
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Abstract

The oxicity assesment of Phenanthrene (PHE) has been investigated by using the rate (r) of survival and population growth in rotifer Brachionus plicatilis. The survival rate was determined after 24 h of exposure to PHE. The survival rate of PHE had no effect at a maximum of $300mg\;L^{-1}$. The r was determined after 72 h of exposure to PHE. It was observed that r in the controls (absence PHE) was greater than 0.5, but that it suddenly decreased with an increased concentration of PHE. PHE reduced r in a dose-dependent manner and a significant reduction occurred at a concentration of greater than $37.5mg\;L^{-1}$. The $EC_{50}$ value of r in PHE exposure was $63.7mg\;L^{-1}$. The no-observed-effect-concentration (NOEC) of r in PHE exposure was $18.8mg\;L^{-1}$. The lowest-observed-effect-concentration (LOEC) of r in the PHE exposure was $37.5mg\;L^{-1}$. From the results, the concentration of PHE (greater than $37.5mg\;L^{-1}$) has a toxic effect on the r of B. plicatilis in natural ecosystems. These results(including NOEC, LOEC and $EC_{50}$) might be useful for the Polycyclic aromatic hydrocarbons(PAHs) toxicity assessment in marine ecosystems.

해산로티퍼(Brachionus plicatilis)의 생존율 및 개체군 성장률을 사용하여 Phenanthrene (PHE)에 대한 독성평가를 수행하였다. PHE에 24시간 노출한 생존율은 최고 농도 $300mg\;L^{-1}$에서 영향이 나타나지 않았다. PHE에 72시간 노출한 개체군 성장률은 $37.5mg\;L^{-1}$ 농도에서 급격한 감소가 시작되어 최고 농도 $150.0mg\;L^{-1}$에서 개체군 성장이 나타나지 않아, 농도의존적으로 감소하는 경향을 보였다. PHE에 노출된 개체군 성장률의 $EC_{50}$ 값은 $63.7(48.4{\sim}84.5)mg\;L^{-1}$, PHE에 대한 개체군 성장률의 NOEC는 $18.8mg\;L^{-1}$, LOEC는 $37.5mg\;L^{-1}$로 나타났다. 연구결과 PHE를 평가하는 데 있어 생존율보다 개체군 성장률이 적합하며 해양환경에서 PHE의 LOEC 이상의 농도는 B. plicatilis에게 독성영향을 미칠 수 있는 농도로 판단된다. 또한 NOEC와 $EC_{50}$은 독성을 평가하는 기준점으로 이용할 수 있으며, 차후 PAHs의 통합적인 생물영향을 판단하기 위한 기초자료로 해양생태계 평가에 활용될 수 있을 것이다.

Keywords

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