대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
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택사와 alisol B acetate의 병용 투여가 천식 동물 모델에 미치는 영향

Anti-asthmatic Effect of Alismatis Rhizoma and Alisol Acetate B Combination Therapy in a Murine Asthma Model

  • 박미준 (부산대학교 한의학전문대학원) ;
  • 허준이 (부산대학교 한의학전문대학원) ;
  • 권민정 (부산대학교 한의학전문대학원) ;
  • 한창우 (부산대학교 한의학전문대학원)
  • Park, Mi-jun (School of Korean Medicine, Pusan National University) ;
  • Heo, June-yi (School of Korean Medicine, Pusan National University) ;
  • Kwun, Min-jung (School of Korean Medicine, Pusan National University) ;
  • Han, Chang-woo (School of Korean Medicine, Pusan National University)
  • 투고 : 2017.09.15
  • 심사 : 2017.12.28
  • 발행 : 2017.12.30
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초록

Objectives: The aim of the study was to evaluate the anti-asthmatic effect of alismatis rhizoma and alisol acetate B combination therapy in a murine asthma model. Methods: C57BL/6 mice were sensitized to and challenged with a mixture of ragweed, dust mite, and aspergillus to induce an asthma animal model. Alismatis rhizoma extract and alisol acetate B combination therapy was co-administered only in the experimental group. To evaluate the anti-asthmatic effect of the combination therapy, inflammatory cell counts in bronchoalveolar lavage (BAL) fluid were determined, and tissue was examined histologically with hematoxylin and eosin (H & E) and periodic acid-Schiff (PAS) stains, by enzyme-linked immunosorbent assay (ELISA) of IgE, IL-4, and IL-5, and with reverse transcription polymerase chain reaction (RT-PCR) of IL-5, IL-33, MUC5AC. Results: Alismatis rhizoma and alisol acetate B combination therapy reduced the number of inflammatory cells, alleviated histologic features, and down-regulated all the investigated asthma mediators, IgE, IL-4, IL-5, IL-33, and MUC5AC. Conclusions: According to the above results, alismatis rhizoma and alisol acetate B combination therapy may have therapeutic potential for asthma.

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참고문헌

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