Journal of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회지)

Korean Society of Radiopharmaceuticals and Molecular Probes (대한방사성의약품학회)
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Simple measurement the chelator number of antibody conjugates by MALDI-TOF MS

  • Shin, Eunbi (Korea Drug Development Platform using radio-isotope) ;
  • Lee, Ji Woong (Department of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences) ;
  • Lee, Kyo Chul (Department of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences) ;
  • Shim, Jae Hoon (Korea Drug Development Platform using radio-isotope) ;
  • Cha, Sangwon (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Kim, Jung Young (Korea Drug Development Platform using radio-isotope)
  • Received : 2017.09.27
  • Accepted : 2017.12.23
  • Published : 2017.12.30
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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) is one of the powerful methods that enable analysis of small molecules as well as large molecules up to about 500,000 Da without severe fragmentation. MALDI-TOF MS, thus, has been a very useful an analytical tool for the confirmation of synthetic molecules, probing PTMs, and identifying structures of a given protein. In recent nuclear medicine, MALDI-TOF MS liner ion mode helps researcher calculate the average number of chelator(or linkage) per an antibody conjugate, such as DOTA-(or DFO-) trastuzumab for labeling a medical radioisotope. This simple technique can be utilized to improve the labeling method and control the quality at the development of antibody-based radiopharmaceuticals, which is very effected to diagnosis and therapy for in vivo tumor cells, with radioisotopes like $^{89}Zr$, $^{64}Cu$, and 177Lu. To minimize the error, MALDI-TOF MS measurement is repeatedly performed for each sample in this study, and external calibration is carried out after data collection.

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References

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