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Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector

  • Kim, Ik-Hyun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Han, Jae-Yeong (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Ju, HyeKyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Moon, Jae Sun (Molecular Biofarming Research Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Hong Gi (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hammond, John (United States Department of Agriculture - Agricultural Research Service, United States National Arboretum, Floral and Nursery Plants Research Unit) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2017.05.31
  • Accepted : 2017.08.08
  • Published : 2017.12.01

Abstract

The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.

Keywords

References

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